2. Others Cell Cycle/DNA Damage
  3. Phosphoramidites DNA/RNA Synthesis Nucleoside Antimetabolite/Analog
  4. DMT-dC(bz) Phosphoramidite

DMT-dC(bz) Phosphoramidite  (Synonyms: DMT-dC(Bz)ホスホロアミダイト)

製品番号: HY-W008849 純度: 99.44%
COA 取扱説明書 Technical Support

DMT-dC (bz) Phosphoramidite is a protected deoxycytidine phosphoramidite monomer, as well as a building block for mechanochemical DNA synthesis. DMT-dC (bz) Phosphoramidite serves as a reagent for oligonucleotide synthesis.

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DMT-dC(bz) Phosphoramidite

DMT-dC(bz) Phosphoramidite 構造式

CAS 番号 : 102212-98-6

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
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5 g $85 在庫あり
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DMT-dC(bz) Phosphoramidite 関連抗体

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DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

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製品説明

DMT-dC (bz) Phosphoramidite is a protected deoxycytidine phosphoramidite monomer, as well as a building block for mechanochemical DNA synthesis. DMT-dC (bz) Phosphoramidite serves as a reagent for oligonucleotide synthesis[1][2].

体外実験

DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz) undergoes mechanochemical coupling with 3'-O-tert-butyldimethylsilyl thymidine to generate the target phosphite triester intermediate in 80% yield[1].
DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz coupling, 20 min at 30 Hz oxidation, 10 min at 30 Hz detritylation) undergoes one-pot mechanochemical coupling, oxidation, and detritylation with 3'-O-tert-butyldimethylsilyl thymidine to generate the target DNA dimer in 32% yield over three steps[1].
DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz coupling, 20 min at 30 Hz sulfurization) undergoes mechanochemical coupling with 3'-O-tert-butyldimethylsilyl thymidine followed by in situ sulfurization to generate the target DNA phosphorothioate dimer in 57% yield over two steps[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DMT-dC(bz) Phosphoramidite 関連抗体
分子量

833.91

分子式

C46H52N5O8P
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

COC(C=C1)=CC=C1C(C2=CC=CC=C2)(C3=CC=C(OC)C=C3)OC[C@@H]4[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)C[C@H](N5C(N=C(NC(C6=CC=CC=C6)=O)C=C5)=O)O4
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (119.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1992 mL 5.9959 mL 11.9917 mL
5 mM 0.2398 mL 1.1992 mL 2.3983 mL
10 mM 0.1199 mL 0.5996 mL 1.1992 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

V1

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (3.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.44%

COA (262 KB) HNMR (301 KB) CNMR (220 KB) RP-HPLC (246 KB) MS (69 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1992 mL 5.9959 mL 11.9917 mL 29.9793 mL
5 mM 0.2398 mL 1.1992 mL 2.3983 mL 5.9959 mL
10 mM 0.1199 mL 0.5996 mL 1.1992 mL 2.9979 mL
15 mM 0.0799 mL 0.3997 mL 0.7994 mL 1.9986 mL
20 mM 0.0600 mL 0.2998 mL 0.5996 mL 1.4990 mL
25 mM 0.0480 mL 0.2398 mL 0.4797 mL 1.1992 mL
30 mM 0.0400 mL 0.1999 mL 0.3997 mL 0.9993 mL
40 mM 0.0300 mL 0.1499 mL 0.2998 mL 0.7495 mL
50 mM 0.0240 mL 0.1199 mL 0.2398 mL 0.5996 mL
60 mM 0.0200 mL 0.0999 mL 0.1999 mL 0.4997 mL
80 mM 0.0150 mL 0.0749 mL 0.1499 mL 0.3747 mL
100 mM 0.0120 mL 0.0600 mL 0.1199 mL 0.2998 mL
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DMT-dC(bz) Phosphoramidite Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMT-dC(bz) Phosphoramidite102212-98-6PhosphoramiditesDNA/RNA SynthesisNucleoside Antimetabolite/Analogoligonucleotide synthesisDNA phosphorothioate dimerDNA dimermechanochemical DNA synthesis3'-O-tert-butyldimethylsilyl thymidinephosphite triester intermediatedeoxycytidine phosphoramidite monomerInhibitorinhibitorinhibit

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製品名:
DMT-dC(bz) Phosphoramidite
製品番号:
HY-W008849
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