2. PROTAC Cell Cycle/DNA Damage Autophagy Apoptosis Immunology/Inflammation
  3. PROTACs FKBP
  4. FKBP12 PROTAC dTAG-13

dTAG-13  (Synonyms: FKBP12 PROTAC dTAG-13)

製品番号: HY-114421 純度: 99.89%
COA 取扱説明書 Technical Support

FKBP12 PROTAC dTAG-13 (dTAG-13), a PROTAC-based heterobifunctional degrader, is a selective degrader of FKBP12F36V with expression of FKBP12F36V in-frame with a protein of interest. FKBP12 PROTAC dTAG-13 effectively engages FKBP12F36V and CRBN, thereby selectively degrading FKBP12F36V.

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CAS 番号 : 2064175-41-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1015 在庫あり
Solution
10 mM * 1 mL in DMSO USD 1015 在庫あり
Solid
1 mg $261 在庫あり
5 mg $550 在庫あり
10 mg $880 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 9 publication(s) in Google Scholar

dTAG-13 関連抗体

Top Publications Citing Use of Products

    FKBP12 PROTAC dTAG-13 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jul 11.  [Abstract]

    HEK-293T cells expressing WDR7-dTAG-3xHA were pretreated with or without dTAG13 (1 μM, 0-60 min). Cell lysates were analyzed by SDS–PAGE and immunoblotting with the indicated antibodies.

    FKBP12 PROTAC dTAG-13 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jul 11.  [Abstract]

    HEK-293T cells expressing endogenous WDR7-dTAG-3xHA and stably expressing the antiporter VMAT2 were pretreated with or without dTAG13 (1 µM, 4 h).

    FKBP12 PROTAC dTAG-13 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2024 Sep 18;15(1):310.  [Abstract]

    dTAG-13 (1 μM, 24 h). Expression, re-localization, and degradation of HA-dTAG Vimentin were monitored by immunofluorescence and quantified by automated image analysis. Scale bar = 500 μm.

    FKBP12 PROTAC dTAG-13 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Nov;13(11):4523-4534.

    293T FBXO44 KO cells were transfected with FLAG-PXR and MYC-FKBPF36V-FBXO44 for 48 h, treated with the indicated doses (0-1 μM) of dTAG-13 for 24 h, and analyzed by Western blot

    FKBP12 PROTAC dTAG-13 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Nov;13(11):4523-4534.

    293T FBXO44 KO cells were transfected with FLAG-PXR and MYC-FKBPF36V-FBXO44 for 48 h, treated with the indicated doses (0-1 μM) of dTAG-13 for 24 h, and analyzed by RT-qPCR.

    PROTACs アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

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    製品説明

    FKBP12 PROTAC dTAG-13 (dTAG-13), a PROTAC-based heterobifunctional degrader, is a selective degrader of FKBP12F36V with expression of FKBP12F36V in-frame with a protein of interest. FKBP12 PROTAC dTAG-13 effectively engages FKBP12F36V and CRBN, thereby selectively degrading FKBP12F36V[1].

    IC50 & Target[1]

    Cereblon

     

    FKBP12

     

    FKBP12(F36V)

     

    体外実験

    TAG-13 (1-1000 nM; 4 hours; 293FTWT cells) treatment potently reduces FKBP12F36V-Nluc levels in 293FTWT cell, indicating the requirement of CRBN for the observed effects[1].
    Treatment of MV4;11 cells expressing BRD4(short)-FKBP12F36V with dTAG-13 leads to robust degradation of BRD4. dTAG-13 treatment leads to rapid degradation of BRD4 within one hou. dTAG-13 treatment leads to rapid and potent degradation of the BRD4 fusion chimera in the heterozygous and homozygous knock-in clones, with no effect on endogenous FKBP12WT[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    dTAG-13 関連抗体

    Western Blot Analysis[1]

    Cell Line: 293FTWT cells
    Concentration: 1 nM, 10 nM, 100 nM, 1000 nM
    Incubation Time: 4 hours
    Result: Potently reduced FKBP12F36V-Nluc levels in 293FTWT cell.
    体内実験

    Following bone marrow engraftment of MV4;11 cells expressing luc-FKBP12F36V in mice, the bioluminescent signal after vehicle or dTAG-13 administration is monitored. A significant, rapid, and durable effect on bioluminescent signal is observed four hours after dTAG-13 administration, indicating effective degradation of luc-FKBP12F36V. Twenty-eight hours following the final treatment, the recovery of cellular bioluminescence to levels comparable between vehicle and dTAG-13 treatment groups is observed[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    1049.17

    分子式

    C57H68N4O15
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    COC1=CC=C(CC[C@H](C2=C(OCC(NCCCCCCOC3=CC=CC4=C3C(N(C5CCC(NC5=O)=O)C4=O)=O)=O)C=CC=C2)OC([C@@H]6CCCCN6C([C@@H](CC)C7=CC(OC)=C(OC)C(OC)=C7)=O)=O)C=C1OC
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (95.31 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9531 mL 4.7657 mL 9.5313 mL
    5 mM 0.1906 mL 0.9531 mL 1.9063 mL
    10 mM 0.0953 mL 0.4766 mL 0.9531 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (2.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.89%

    COA (248 KB) HNMR (148 KB) RP-HPLC (169 KB) LCMS (233 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.9531 mL 4.7657 mL 9.5313 mL 23.8284 mL
    5 mM 0.1906 mL 0.9531 mL 1.9063 mL 4.7657 mL
    10 mM 0.0953 mL 0.4766 mL 0.9531 mL 2.3828 mL
    15 mM 0.0635 mL 0.3177 mL 0.6354 mL 1.5886 mL
    20 mM 0.0477 mL 0.2383 mL 0.4766 mL 1.1914 mL
    25 mM 0.0381 mL 0.1906 mL 0.3813 mL 0.9531 mL
    30 mM 0.0318 mL 0.1589 mL 0.3177 mL 0.7943 mL
    40 mM 0.0238 mL 0.1191 mL 0.2383 mL 0.5957 mL
    50 mM 0.0191 mL 0.0953 mL 0.1906 mL 0.4766 mL
    60 mM 0.0159 mL 0.0794 mL 0.1589 mL 0.3971 mL
    80 mM 0.0119 mL 0.0596 mL 0.1191 mL 0.2979 mL
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    FKBP12 PROTAC dTAG-13 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FKBP12 PROTAC dTAG-132064175-41-1dTAG-13dTAG13dTAG 13PROTACsFKBPFK506-binding proteinBegradationCRBNFKBP12F36Vheterodimerizers rapidBRD4Inhibitorinhibitorinhibit

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    製品名:
    FKBP12 PROTAC dTAG-13
    製品番号:
    HY-114421
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