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  4. Grape seed extract

Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells.

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Grape seed extract Chemical Structure

Grape seed extract Chemical Structure

CAS No. : 84929-27-1

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Grape seed extract Related Antibodies

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Description

Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells[1][2][3].

In Vitro

Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells[3].
Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells[3].
Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Grape seed extract Related Antibodies

Cell Proliferation Assay[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours, 48 hours
Result: Inhibited cell growth in a dose- and a time-dependent manner.

Cell Cycle Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours, 48 hours
Result: Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases).

Western Blot Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours
Result: Modulates the protein levels of CDKIs, CDKs, and cyclins.

Apoptosis Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 24 hours
Result: Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells.
In Vivo

Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice[3].
Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors[3].
Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft[3]
Dosage: 200 mg/kg
Administration: Oral gavage, 5 days/wk, for 8 weeks
Result: Showed time-dependent inhibition of tumor growth.
Clinical Trial
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

[Grape seed extract]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 6 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution

    This protocol yields a clear solution of ≥ 0.6 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution

    This protocol yields a clear solution of ≥ 0.6 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
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%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
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Grape seed extract Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Grape seed extract84929-27-1Apoptosisnutraceuticalagentanti-inflammatoryanti-proliferativeInhibitorinhibitorinhibit

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