2. MCE Kits
  3. Protein Biology
  4. Protein Purification
  5. Magnetic Beads
  6. Anti-c-Myc Magnetic Beads

Anti-c-Myc Magnetic Beads 

Cat. No.: HY-K0206
Manual COA SDS Technical Support
Magnetic Beads

MCE Anti-c-Myc Magnetic Beads are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

사이즈 가격 재고 수량
무료 샘플 (100 μL)   지금 신청하기  
1 mL 해외재고보유
5 mL 해외재고보유
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

77 Publications Citing Use of MCE Anti-c-Myc Magnetic Beads

WB
IP

    Anti-c-Myc Magnetic Beads purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 9;10(1):217.  [Abstract]

    IP detection of protein expression in HEK293T cells.

    Anti-c-Myc Magnetic Beads purchased from MCE. Usage Cited in: Nat Biomed Eng. 2024 Dec;8(12):1634-1650.  [Abstract]

    Representative immunoblot analysis of immunoprecipitation of EGFR binder and scFv.

    Anti-c-Myc Magnetic Beads purchased from MCE. Usage Cited in: Sci Bull. 2023 Sep 30;68(18):2094-2105.

    IP detection of TARS2 expression in HEK293T cells.

    Anti-c-Myc Magnetic Beads purchased from MCE. Usage Cited in: Mol Cell. 2023 Dec 7;83(23):4370-4385.e9.  [Abstract]

    Autopalmitoylation levels of ZDHHC4-Myc-FL and DC3 mutant in 293T cells.

    Anti-c-Myc Magnetic Beads purchased from MCE. Usage Cited in: Cell Death Differ. 2020 Jun;27(6):1981-1997.  [Abstract]

    Immunoblotting analysis of the indicated proteins in coimmunoprecipitation assays was performed using anti-Myc magnetic beads in 293T cells expressing the indicated plasmids.

    • 제품 설명

    • 보관

    • Protocol

    • 구성요소

    • 각종 서류

    Description
    & Advantages

    Anti-c-Myc Magnetic Beads are based on amino magnetic beads, with 200 nm particle size, covalently coupling with high quality mouse IgG1 monoclonal antibody that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-Myc protein.

     

    1. During immunoprecipitation, only a small amount of magnetic beads are needed.

    2. Convenient and time saving

    3. Low non-specific binding

    4. Minimal sample loss

    5. Protein binding capacity up to 0.5 mg/mL

    6. Stable, one bottle solution
    보관

    4°C, 2 years.

    Do not centrifuge, dry or freeze the magnetic beads.
    Protocol

    1. Preparation of Magnetic Beads

    1.1 Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times, do not vortex). Transfer 20 μL of Anti-c-Myc Magnetic Beads suspension into a new tube.

    1.2 Add 500 μL of wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand (MCE Cat. No.: HY-K0200) to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 2 times

    2. Protein Binding

    2.1 Add 500 μL of cell lysate (the sample containing c-Myc-tagged protein) to the washed beads. For Ag binding, incubate for 2 hours at room temperature or overnight at 4°C while gently rotating the tube.

    2.2 Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant.

    NOTE: Occasional aggregation of magnetic beads during the binding process doesn’t affect experimental results.

    3. Washing

    Add 500 μL of wash buffer to the Magbeads-Ag complex and mix gently. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

    4. Elution & Detection

    Add 50 μL of 1× SDS-PAGE loading buffer into the tube with Magbeads-Ag complex and boil for 5 minutes. Cool and place the tube into a magnetic stand to collect the beads against the side of the tube. Keep the supernatant containing the target antigen for SDS-PAGE analysis.
    구성요소
    Components HY-K0206-1 mL HY-K0206-5 mL
    Anti-c-Myc Magnetic Beads 1 mL 1 mL×5
    각종 서류

    Manual (264 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Anti-c-Myc immunomagnetic beadsc-Myc antibody magnetic beads

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    상품명:
    Anti-c-Myc Magnetic Beads
    Cat. No.:
    HY-K0206
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