Anti-c-Myc Magnetic Beads 

Anti-c-Myc Magnetic Beads are based on amino magnetic beads, with 200 nm particle size, covalently coupling with high quality mouse IgG₁ monoclonal antibody that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-Myc protein.

1. During immunoprecipitation, only a small amount of magnetic beads are needed.

2. Convenient and time saving

3. Low non-specific binding

4. Minimal sample loss

5. Protein binding capacity up to 0.5 mg/mL

6. Stable, one bottle solution

4°C, 2 years.

Do not centrifuge, dry or freeze the magnetic beads.

1. Preparation of Magnetic Beads

1.1 Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times, do not vortex). Transfer 20 μL of Anti-c-Myc Magnetic Beads suspension into a new tube.

1.2 Add 500 μL of wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand (MCE Cat. No.: HY-K0200) to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 2 times

2. Protein Binding

2.1 Add 500 μL of cell lysate (the sample containing c-Myc-tagged protein) to the washed beads. For Ag binding, incubate for 2 hours at room temperature or overnight at 4°C while gently rotating the tube.

2.2 Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant.

NOTE: Occasional aggregation of magnetic beads during the binding process doesn’t affect experimental results.

3. Washing

Add 500 μL of wash buffer to the Magbeads-Ag complex and mix gently. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

4. Elution & Detection

Add 50 μL of 1× SDS-PAGE loading buffer into the tube with Magbeads-Ag complex and boil for 5 minutes. Cool and place the tube into a magnetic stand to collect the beads against the side of the tube. Keep the supernatant containing the target antigen for SDS-PAGE analysis.