2. MCE-Kits
  3. Protein Biology
  4. Protein Sample Preparation
  5. Cell Lysis
  6. NP-40 Lysis Buffer

NP-40 Lysis Buffer 

Art. -Nr.: HY-K1002
Manual COA SDS Technical Support
Expression Purification

MCE NP-40 Lysis Buffer is a relatively mild reliable buffers used to lyse cells from animal, plant tissue and fungi, bacteria etc.

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  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

MCE NP-40 Lysis Buffer is a relatively mild lysis solution that can be used for animal and plant cells or tissues, as well as fungal and bacterial samples. The protein samples obtained by lysis can be used for PAGE, WB, IP, Co-IP, ELISA and other experiments, etc. Mild lysis is benefit for maintaining the original protein structure and the original protein-protein interactions.

NP-40 Lysis Buffer contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40 and a variety of inhibitors such as sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, sodium fluoride, EDTA and leupeptin, etc., which can effectively inhibit protein degradation.
Speicherung

-20℃, 1 year.
Komponenten
Components HY-K1002-100 mL HY-K1002-500 mL
NP-40 Lysis Buffer 100 mL 100 mL × 5
Dokumentation

Manual (118 KB) SDS (132 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NP40 Cell Lysis BufferProtein Lysis BufferWB Lysis Buffer

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NP-40 Lysis Buffer
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