2. MCE Kits
  3. Molecular Biology
  4. RT-PCR
  5. Reverse transcription PCR
  6. RT Master Mix for qPCR III

RT Master Mix for qPCR III 

Cat. No.: HY-K0513
Manual COA SDS Technical Support

MCE MCE Reverse Transcription Kit III is suitable for the conventional synthesis of cDNA from RNA and the stem-loop method for the synthesis of cDNA from microRNA (miRNA).

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1 Publications Citing Use of MCE RT Master Mix for qPCR III

  • Descripciòn

  • Almacenamiento

  • Componentes

  • Documentación

Description
& Advantages

MCE MCE Reverse Transcription Kit III is suitable for the conventional synthesis of cDNA from RNA and the stem-loop method for the synthesis of cDNA from microRNA (miRNA). The kit contains third-generation M-MLV reverse transcriptase and various components required for reverse transcription to synthesize cDNA. Simply add the RNA template, primers, and water to start the reaction. Different types of reverse transcription primers can be flexibly used depending on the experimental design and up to 12 kb of cDNA can be obtained within 15 min.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501, HY-K0501A) is highly recommended for detection of the expression levels of interested genes.
Almacenamiento

-20℃，2 years

Protect from light and avoid repeated freeze-thaw cycles
Componentes
Components HY-K0513-100 rxns
RNA RT Mix 400 μL
10× dsDNase Buffer 200 μL
Oligo(dT)20VN (50 μM) 100 μL
Random hexamers (50 μM) 100 μL
dsDNase 50 μL × 2
Nuclease-Free H2O 1 mL × 2
Documentación

Manual (3536 KB) SDS (251 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
RT Master Mix for qPCR III
Cat. No.:
HY-K0513
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