RT Master Mix for qPCR III 

MCE MCE Reverse Transcription Kit III is suitable for the conventional synthesis of cDNA from RNA and the stem-loop method for the synthesis of cDNA from microRNA (miRNA). The kit contains third-generation M-MLV reverse transcriptase and various components required for reverse transcription to synthesize cDNA. Simply add the RNA template, primers, and water to start the reaction. Different types of reverse transcription primers can be flexibly used depending on the experimental design and up to 12 kb of cDNA can be obtained within 15 min.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501, HY-K0501A) is highly recommended for detection of the expression levels of interested genes.

-20℃，2 years

Protect from light and avoid repeated freeze-thaw cycles