2. 형광 염료

형광 염료

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

형광 염료 (3782):

Cat. No. 상품명 CAS No. Purity 화학구조
  • HY-D0026
    AMCA,SE 113721-87-2
    AMCA,SE (7-Amino-4-methyl-3-coumarinacetic acid N-succinimidyl ester) is a bright blue fluorescent dye. AMCA,SE reacts with amino groups and is excited by ultraviolet light. AMCA,SE can be used for amine reactive labeling (Ex/Em = 354/440 nm).
    AMCA,SE
  • HY-D0202
    Arsenazo III 1668-00-4
    Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca2+, Mg2+, Sr2+, Ba2+, K+, and Na+, and its binding affinity for Ca2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca2+ in cells.
    Arsenazo III
  • HY-D0331
    Reactive Blue 19 2580-78-1
    Reactive Blue 19 is an anthraquinone dye that can be used in the textile industry.
    Reactive Blue 19
  • HY-D0813
    Zincon sodium 62625-22-3
    Zincon sodium is an excellent colorimetric reagent for the detection of zinc and copper ions in aqueous solution. Zincon sodium can be used for the determination of zinc, copper, and cobalt ions in metalloproteins.
    Zincon sodium
  • HY-D1090
    ​JOE 82855-40-1
    JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing.
    ​JOE
  • HY-D1171
    Fluorescent brightener 220 16470-24-9 98.0%
    Fluorescent brightener 220 is an optical brightener dye.
    Fluorescent brightener 220
  • HY-D1208
    Alkaline phosphatase substrate 160081-62-9
    Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays.
    Alkaline phosphatase substrate
  • HY-D1256
    Msr-blue 2966537-39-1 99.38%
    Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm).
    Msr-blue
  • HY-D1262
    BODIPY-TS 1808174-52-8
    BODIPY-TS (Thiol-green 2) is a fast response and thiol-specific turn-on probe. BODIPY-TS utilizes the thiosulfonate scaffold as a thiol recognition unit. BODIPY-TS has low toxicity, and features high selectivity, low detection limit, and quantitative reaction to thiols. Ex: 490 nM; Em: 515 nM.
    BODIPY-TS
  • HY-D1321
    Cy 5 amine hydrochloride 1807529-70-9
    Cyanine5 amine hydrochloride is a potent fluorescent dye. Cyanine5 amine hydrochloride can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). (λex=646 nm, λem=662 nm).
    Cy 5 amine hydrochloride
  • HY-D1464
    CH1055 1622250-38-7
    CH1055 is a near-infrared II (NIR-II) fluorescent dye that can be used as a fluorescent probe for in vivo imaging. CH1055 can be conjugated with antibodies or ligands for specific imaging (for example the tumor imaging).
    CH1055
  • HY-D1550
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515 886212-87-9 98.78%
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515 is a fluorescent dye for Cu2+ ion.
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515
  • HY-D1557
    Cyanine5.5 tetrazine
    Cyanine5.5 tetrazine is a far-infrared luminescent dye. Cyanine5.5 tetrazine is a Cyanine5.5 (HY-D0925A) derivative contains a tetrazine moiety. Cyanine5.5 tetrazine can be used for in vivo imaging and low background applications. Cyanine5.5 tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
    Cyanine5.5 tetrazine
  • HY-D1603
    BODIPY FL-EDA 209541-26-4 99.23%
    BODIPY FL-EDA is a widely used fluorescent dye for quantitative analysis of nucleotides. BODIPY FL-EDA is an aliphatic amine analog that can react with aldehydes and ketones. BODIPY FL-EDA can be used to detect both modified and unmodified deoxynucleotides and to determine DNA damage and genomic DNA methylation through capillary electrophoresis with laser-induced fluorescence (CE-LIF). Additionally, it can be used for quantifying intracellular ATP levels. The excitation wavelength is 500 nm, and the emission wavelength is 510 nm.
    BODIPY FL-EDA
  • HY-D1634
    Bz-FVR-AMC 88899-22-3 99.83%
    Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM-1s-1. The high concentration of BZ-FVR-AMC inhibits the substrate.
    Bz-FVR-AMC
  • HY-D1832
    Vari Fluor 532 TSA (200×)
    Vari Fluor 532 TSA (200×) (VF 532 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 532 TSA (200×)
  • HY-D1873
    800CW acid 1088919-86-1 98.81%
    800CW acid is an orally active near-infrared (NIR) dye (excitation/emission 774/789 nm). 800CW acid can be used for intestinal permeability testing. 800CW acid can be used for in vivo imaging studies.
    800CW acid
  • HY-D1880
    Sulfo-Cy5 hydrazide 2055138-61-7
    Sulfo-Cy5 hydrazide is a fluorescent dye containing a hydrazide functionality (Ex/Em=646/662 nm).
    Sulfo-Cy5 hydrazide
  • HY-D2000
    ATTO 488 923585-42-6 99.00%
    ATTO 488 is a hydrophilic rhodamine-based fluorescent dye with strong absorption and high fluorescence quantum yield. ATTO 488 is suitable for flow cytometry (FACS), fluorescence in situ hybridization (FISH) and most other labeling uses (Ex/Em: 500/520 nm).
    ATTO 488
  • HY-D2175
    AF 555 NHS ester
    AF 555 NHS ester is a bright, orange-red fluorescence probe. AF 555 NHS ester can be used to label primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules (Ex/Em = 552/566 nm).
    AF 555 NHS ester