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Cat. No.: HY-D1208

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Alkaline phosphatase substrate

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Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays.

For research use only. We do not sell to patients.

Purity: 95.30%
CAS No.: 160081-62-9
Formula: C₁₈H₁₉Cl₂Na₂O₇P
Molecular Weight:495.20

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Alkaline phosphatase substrate Chemical Structure

CAS No.: 160081-62-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays^[1].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Mix the substance to be tested (phosphatase mimic) with the chemiluminescent substrate Alkaline phosphatase substrate (25 μM) in 20 mM HEPES buffer (pH 8.5).
2. Immediately measure the chemiluminescence intensity of the mixture at room temperature.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 160081-62-9
Appearance Liquid
Molecular Weight 495.20
Formula C₁₈H₁₉Cl₂Na₂O₇P
Color Colorless to light yellow
SMILES

COC1(C(C=C2)=CC(O[P](O[Na])(O[Na])=O)=C2Cl)[C@]([C@H]3C4)(OO1)[C@@H](C[C@H]5C3)C[C@]4(C5)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hu L, et al. Chemiluminescence of the Ce(IV)/CDP-Star System Based on the Phosphatase-like Activity of Ce(IV) Ions. ACS Omega. 2021 Feb 25;6(9):6379-6384. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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