Bz-FVR-AMC
Based on 1 Customer Validation
Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a kcat/Km value of 1070 mM-1s-1. The high concentration of BZ-FVR-AMC inhibits the substrate.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 88899-22-3
- Formula: C37H43N7O6
- Molecular Weight:681.78
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Processing and activation of procathepsin S[2]:
1. Autocatalytic activation of procathepsin S was studied by incubation (final concentration 1-5 μM) at 37 °C in 0.5 mL of the appropriate buffer containing 2.5 mM dithiothreitol.
2. Aliquots of 10 μl were taken from the reaction mixture at the times indicated and mixed with 2.5 ml of substrate solution (10 μM Bz-FVR-AMC in 0.1 M phosphate buffer, pH 6.5, containing 1 mM EDTA (HY-Y0682) and 0.1% (w/v) polyethylene glycol 6000).
3. Fluorescence of the released AMC was then monitored continuously for 1 min in a pectrofluorimeter at excitation and emission wavelengths of 370 and 460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 88899-22-3
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Appearance Solid
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Molecular Weight 681.78
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Formula C37H43N7O6
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Color White to off-white
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SMILES
CC1=CC(OC2=CC(NC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC(C3=CC=CC=C3)=O)CC4=CC=CC=C4)=O)=O)CCCNC(N)=N)=O)=CC=C12)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 125 mg/mL (183.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Vasiljeva O, et al. Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans. FEBS Lett. 2005 Feb 14;579(5):1285-90. [Content Brief]
[2]. Vasiljeva O, et al. Recombinant human cathepsin H lacking the mini chain is an endopeptidase. Biochemistry. 2003 Nov 25;42(46):13522-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4667 mL | 7.3337 mL | 14.6675 mL | 36.6687 mL |
| 5 mM | 0.2933 mL | 1.4667 mL | 2.9335 mL | 7.3337 mL | |
| 10 mM | 0.1467 mL | 0.7334 mL | 1.4667 mL | 3.6669 mL | |
| 15 mM | 0.0978 mL | 0.4889 mL | 0.9778 mL | 2.4446 mL | |
| 20 mM | 0.0733 mL | 0.3667 mL | 0.7334 mL | 1.8334 mL | |
| 25 mM | 0.0587 mL | 0.2933 mL | 0.5867 mL | 1.4667 mL | |
| 30 mM | 0.0489 mL | 0.2445 mL | 0.4889 mL | 1.2223 mL | |
| 40 mM | 0.0367 mL | 0.1833 mL | 0.3667 mL | 0.9167 mL | |
| 50 mM | 0.0293 mL | 0.1467 mL | 0.2933 mL | 0.7334 mL | |
| 60 mM | 0.0244 mL | 0.1222 mL | 0.2445 mL | 0.6111 mL | |
| 80 mM | 0.0183 mL | 0.0917 mL | 0.1833 mL | 0.4584 mL | |
| 100 mM | 0.0147 mL | 0.0733 mL | 0.1467 mL | 0.3667 mL |
Bz-FVR-AMC Related Classifications
HY-D1634 Related Classifications
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