Bz-FVR-AMC 

Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a k_cat/K_m value of 1070 mM^-1s^-1. The high concentration of BZ-FVR-AMC inhibits the substrate^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Processing and activation of procathepsin S^[2]:
1. Autocatalytic activation of procathepsin S was studied by incubation (final concentration 1-5 μM) at 37 ℃ in 0.5 mL of the appropriate buffer containing 2.5 mM dithiothreitol.
2. Aliquots of 10 μl were taken from the reaction mixture at the times indicated and mixed with 2.5 ml of substrate solution (10 μM Bz-FVR-AMC in 0.1 M phosphate buffer, pH 6.5, containing 1 mM EDTA (HY-Y0682) and 0.1% (w/v) polyethylene glycol 6000).
3. Fluorescence of the released AMC was then monitored continuously for 1 min in a pectrofluorimeter at excitation and emission wavelengths of 370 and 460 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

681.78

C₃₇H₄₃N₇O₆

88899-22-3

454

344

CC1=CC(OC2=CC(NC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC(C3=CC=CC=C3)=O)CC4=CC=CC=C4)=O)=O)CCCNC(N)=N)=O)=CC=C12)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Vasiljeva O, et al. Recombinant human procathepsin S is capable of autocatalytic processing at neutral pH in the presence of glycosaminoglycans. FEBS Lett. 2005 Feb 14;579(5):1285-90.  [Content Brief]

  [2]. Vasiljeva O, et al. Recombinant human cathepsin H lacking the mini chain is an endopeptidase. Biochemistry. 2003 Nov 25;42(46):13522-8.  [Content Brief]