ATTO 488
Based on 1 Customer Validation
ATTO 488 is a hydrophilic rhodamine-based fluorescent dye with strong absorption and high fluorescence quantum yield. ATTO 488 is suitable for flow cytometry (FACS), fluorescence in situ hybridization (FISH) and most other labeling uses (Ex/Em: 500/520 nm).
For research use only. We do not sell to patients.
- Purity: 99%
- CAS No.: 923585-42-6
- Formula: C25H24ClN3O14S2
- Molecular Weight:690.05
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of ATTO 488 Staining Solution :
Prepare a 10 mM stock solution using DMSO.
2. Marking Process :
Add 2 times the molar amount of excess ATTO 488 to the purified protein.
Note: Conduct marking and spectral measurements in a green-safe light or dark environment, and perform the operation as quickly as possible.
3. Incubation Method:
Incubate at room temperature (RT) for 2 hours or at 4°C in the dark with shaking for overnight.
4. Purification :
1) Use the NAP-5 size exclusion column to remove the free ATTO 488.
2) Remove trace free ATTO 488 through ultrafiltration.
5. Characterize the labeled protein using ultraviolet/visible spectroscopy.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 923585-42-6
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Appearance Solid
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Molecular Weight 690.05
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Formula C25H24ClN3O14S2
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Color Brown to red
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SMILES
O=C(CCCN(C)C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)(O)=O)C=CC=C1)=O)O.O=Cl(=O)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Elkurdi A, et al. Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum. Photochem Photobiol. 2024 May-Jun;100(3):561-572. [Content Brief]
[2]. Konrad A, et al. Revealing the radiative and non-radiative relaxation rates of the fluorescent dye Atto488 in a λ/2 Fabry-Pérot-resonator by spectral and time resolved measurements. Nanoscale. 2016 Aug 14;8(30):14541-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ATTO 488 Related Classifications
HY-D2000 Related Classifications
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