2. 蛍光色素

蛍光色素

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

蛍光色素 (3784):

製品番号 製品名 CAS 番号 純度 構造式
  • HY-D0808
    Meldola blue 7057-57-0
    Meldola blue is a biosensor for measuring lactate in serum based on screen-printed carbon electrodes modified with Meldola's Blue-Reinecke salt (MBRS-SPCE) coated with lactate dehydrogenase NAD+-dependent enzyme (from pig heart) and NAD+.
    Meldola blue
  • HY-D1196
    Direct Blue 71 4399-55-7
    Direct Blue 71 is a good dyeing agent for cotton fabrics and can be adsorbed by palm ash and removed from the aqueous solution.
    Direct Blue 71
  • HY-D2250
    Disulfo-ICG carboxylic acid 2757507-18-7
    Disulfo-ICG carboxylic acid is a derivative of ICG dye. ICG (Indocyanine Green) is a NIR fluorescent dye.
    Disulfo-ICG carboxylic acid
  • HY-D2847B
    FITC-PEG5000-DBCO
    FITC-PEG5000-DBCO is a fluorescent dye composed of FITC (HY-66019), PEG and dibenzocyclooctyne (DBCO). DBCO is a cycloalkyne that can react with molecules containing azide groups through "click chemistry" to achieve coupling (Ex/Em=488/525 nm).
    FITC-PEG5000-DBCO
  • HY-106594B
    Prussian blue soluble 12240-15-2
    Prussian blue soluble is a good adsorbent to be used as antidotes for poisoning with cesium or thallium ions. Prussian blue soluble has anticancerous and antibacterial properties. Prussian blue soluble can be used as a contrast agent in photoacoustic and magnetic resonance imaging (MRI).
    Prussian blue soluble
  • HY-150150F
    Cy3-siRNA Negative Control
    Cy3-siRNA Negative Control is Cy3-labeled SiRNA Negative Control (HY-150150). Cy3-siRNA Negative Control can be used for easy visualization and assessment of transfection efficiency.
    Cy3-siRNA Negative Control
  • HY-W127705
    Quinacrine mustard dihydrochloride 4213-45-0
    Quinacrine mustard dihydrochloride is a fluorochrome. Quinacrine mustard dihydrochloride as a polycyclic aromatic agent can be used as mutagenic agent induces the mutants of bacteria. Quinacrine mustard dihydrochloride induces cell cycle arrest at G2/M-phase. Quinacrine mustard dihydrochloride has the potential for the research of plant, animal, or human chromosomes.
    Quinacrine mustard dihydrochloride
  • HY-W998680
    Fluorescein-maleimide 2228857-33-6
    Fluorescein-maleimide (5-FITC-Maleimide) is a thiol-reactive fluorescent derivatization reagent and non-specific protein labeling reagent. Fluorescein-maleimide covalently binds to protein thiol groups for protein labeling. Fluorescein-maleimide covalently binds to protein amino and imidazole groups under neutral pH conditions. Fluorescein-maleimide is used for fluorescent labeling of proteins, nucleic acids or other molecules containing one or more thiol groups (Ex/Em = 494/519 nm).
    Fluorescein-maleimide
  • HY-D1044
    Rhodamine 101 chloride 64339-18-0
    Rhodamine 101 chloride (Rhodamine 640 chloride) is a fluorescent dye with excitation and emission maxima at 565 nM and 595 nM, respectively. Rhodamine 101 chloride binds to intracellular proteins and emits specific fluorescence. Rhodamine 101 chloride can be used in colon cancer research.
    Rhodamine 101 chloride
  • HY-D1444
    Propidium monoazide

    Propidium monoazide

    		91416-20-5 ≥99.0%
    Propidium monoazide is a cell-impermeant photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Propidium monoazide
  • HY-D0954
    Jenner's Stain 62851-42-7
    Jenner's Stain is a dye that is used in microscopy for staining blood smears. Jenner's Stain can be used for the chromosome stain by C-banding technique. Jenner's Stain can be used for the stain for routine blood examinations and malarial staining.
    Jenner's Stain
  • HY-103594
    2-Aminoacridone

    2-Aminoacridone

    		27918-14-5
    2-Aminoacridone is a widely used fluorophore (λexc=428 nm, λem=525 nm).
    2-Aminoacridone
  • HY-D0004
    Azure B 531-55-5 99.87%
    Azure B is a cationic dye and the major metabolite of Methylene blue. Azure B is used in making Azure eosin stains for blood smear staining. Azure B is a high-potency, selective and reversible inhibitor of monoamine oxidases (MAO)-A, with IC50s of 11 and 968 nM for recombinant human MAO-A and MAO-B, respectively. Azure B possesses significant antidepressant-like effects.
    Azure B
  • HY-D0068
    DEAC, SE 139346-57-9 99.32%
    DEAC, SE (7-Diethylaminocoumarin-3-carboxylic acid, SE) is an excellent blue fluorescent building block for labeling amine-containing biomolecules.
    DEAC, SE
  • HY-D0149
    4-(Trifluoromethyl)umbelliferone 575-03-1 98.69%
    4-(Trifluoromethyl)umbelliferone is fluorescent probe substrate for rat hepatic cytochrome P450 enzymes.
    4-(Trifluoromethyl)umbelliferone
  • HY-D0800
    Mordant Blue 29 1667-99-8
    Chromeazurol S is a compound belonging to the class of azo dyes. It is often used as an indicator in analytical chemistry to detect metal ions such as copper, nickel, and cobalt. Chromeazurol S turns from yellow to blue in the presence of metal ions, allowing them to be detected and quantified. It can be applied to a test strip or added directly to a solution for analysis.
    Mordant Blue 29
  • HY-D0853
    DiAzKs

    DiAzKs

    		1253643-88-7 99.93%
    DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe.
    DiAzKs
  • HY-D0957
    Ethyl Violet 2390-59-2
    Ethyl Violet is a triphenylmethane cationic dye with antibacterial activity. Ethyl Violet is applicable to research related to antibacterial therapy and histological staining.
    Ethyl Violet
  • HY-D1174
    Rhodamine 700 63561-42-2 98.50%
    Rhodamine 700, a Lambdachrome laser dye, is one of the few rhodamine dyes with near infrared fluorescence. Rhodamine 700 can be used for mitochondrial staining.
    Rhodamine 700
  • HY-D1240
    Rhodamine 101 inner salt 116450-56-7 98.1%
    Rhodamine 101 inner salt is a bright fluorescent dye with excitation and emission maxima at 565 and 595 nm, respectively. It can be used in various biological applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA.
    Rhodamine 101 inner salt