DiAzKs
Based on 1 publication(s) in Google Scholar
DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 1253643-88-7
- Formula: C11H20N4O4
- Molecular Weight:272.30
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DiAzKs
More
Biological Activity
Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1253643-88-7
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Appearance Solid
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Molecular Weight 272.30
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Formula C11H20N4O4
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Color White to yellow
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SMILES
N[C@@H](CCCCNC(OCCC1(C)N=N1)=O)C(O)=O
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Synonyms
H-L-Photo-lysine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
2021 Mar;39(3):347-356. PMID: 33077962
Solvent & Solubility
H2O : 5 mg/mL (18.36 mM; Need ultrasonic)
DMSO : 2 mg/mL (7.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
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Handling Instructions (2659 KB)
References
[1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7. [Content Brief]
[2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. [Content Brief]
[3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL | 91.8105 mL |
| 5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL | 18.3621 mL | |
| H2O | 10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL | 9.1811 mL |
| 15 mM | 0.2448 mL | 1.2241 mL | 2.4483 mL | 6.1207 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DiAzKs Related Classifications
HY-D0853 Related Classifications
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