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DiAzKs hydrochloride  (Synonyms: H-L-Photo-lysine hydrochloride)

Cat. No.: HY-D0853A Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe.

For research use only. We do not sell to patients.

DiAzKs hydrochloride

DiAzKs hydrochloride Chemical Structure

CAS No. : 2421187-79-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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Based on 1 publication(s) in Google Scholar

Other Forms of DiAzKs hydrochloride:

DiAzKs hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE DiAzKs hydrochloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe[1][2][3].

In Vitro

Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DiAzKs hydrochloride Related Antibodies
Molecular Weight

308.76

Formula

C11H21ClN4O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCCNC(OCCC1(C)N=N1)=O)C(O)=O.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (809.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (161.94 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2388 mL 16.1938 mL 32.3876 mL
5 mM 0.6478 mL 3.2388 mL 6.4775 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.0%

SDS (604 KB)

COA (244 KB) HNMR (278 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.2388 mL 16.1938 mL 32.3876 mL 80.9690 mL
5 mM 0.6478 mL 3.2388 mL 6.4775 mL 16.1938 mL
10 mM 0.3239 mL 1.6194 mL 3.2388 mL 8.0969 mL
15 mM 0.2159 mL 1.0796 mL 2.1592 mL 5.3979 mL
20 mM 0.1619 mL 0.8097 mL 1.6194 mL 4.0485 mL
25 mM 0.1296 mL 0.6478 mL 1.2955 mL 3.2388 mL
30 mM 0.1080 mL 0.5398 mL 1.0796 mL 2.6990 mL
40 mM 0.0810 mL 0.4048 mL 0.8097 mL 2.0242 mL
50 mM 0.0648 mL 0.3239 mL 0.6478 mL 1.6194 mL
60 mM 0.0540 mL 0.2699 mL 0.5398 mL 1.3495 mL
80 mM 0.0405 mL 0.2024 mL 0.4048 mL 1.0121 mL
100 mM 0.0324 mL 0.1619 mL 0.3239 mL 0.8097 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DiAzKs hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DiAzKs2421187-79-1H-L-Photo-lysineFluorescent DyeDiazirinePTMsphoto-cross-linkerprotein-proteininteractionsUVInhibitorinhibitorinhibit

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