DiAzKs hydrochloride
Based on 1 publication(s) in Google Scholar
DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2421187-79-1
- Formula: C11H21ClN4O4
- Molecular Weight:308.76
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DiAzKs hydrochloride
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Biological Activity
Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2421187-79-1
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Appearance Solid
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Molecular Weight 308.76
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Formula C11H21ClN4O4
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Color White to off-white
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SMILES
N[C@@H](CCCCNC(OCCC1(C)N=N1)=O)C(O)=O.[H]Cl
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Synonyms
H-L-Photo-lysine hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
2021 Mar;39(3):347-356. PMID: 33077962
Solvent & Solubility
DMSO : 250 mg/mL (809.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (161.94 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Korean - KR (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
[1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7. [Content Brief]
[2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. [Content Brief]
[3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.2388 mL | 16.1938 mL | 32.3876 mL | 80.9690 mL |
| 5 mM | 0.6478 mL | 3.2388 mL | 6.4775 mL | 16.1938 mL | |
| 10 mM | 0.3239 mL | 1.6194 mL | 3.2388 mL | 8.0969 mL | |
| 15 mM | 0.2159 mL | 1.0796 mL | 2.1592 mL | 5.3979 mL | |
| 20 mM | 0.1619 mL | 0.8097 mL | 1.6194 mL | 4.0485 mL | |
| 25 mM | 0.1296 mL | 0.6478 mL | 1.2955 mL | 3.2388 mL | |
| 30 mM | 0.1080 mL | 0.5398 mL | 1.0796 mL | 2.6990 mL | |
| 40 mM | 0.0810 mL | 0.4048 mL | 0.8097 mL | 2.0242 mL | |
| 50 mM | 0.0648 mL | 0.3239 mL | 0.6478 mL | 1.6194 mL | |
| 60 mM | 0.0540 mL | 0.2699 mL | 0.5398 mL | 1.3495 mL | |
| 80 mM | 0.0405 mL | 0.2024 mL | 0.4048 mL | 1.0121 mL | |
| 100 mM | 0.0324 mL | 0.1619 mL | 0.3239 mL | 0.8097 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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