Other Analysis Dyes

Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography (Ex/Em = 785/813 nm).

Dansyl chloride is a reagent that produces stable blue or blue-green fluorescent sulfonamide adducts in the reaction of aliphatic and aromatic amines with primary amino groups, and is widely used for modified amino acids, protein sequencing and amino acid analysis.

Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride ([Ru(dpp)3]²⁺) is an electrochemiluminescent (ECL) probe and oxygen-sensitive sensor. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride can be used to modify electrode surfaces for the detection of sulfates (S₂O₈^2-) and oxalates, based on electrochemical reactions that generate excited-state species, releasing photons through irreversible redox reactions. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride utilizes the oxygen quenching of fluorescence mechanism, where fluorescence intensity reflects the metabolic rate of living microorganisms or oxygen levels within cells/tumors, allowing for real-time detection. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride's main applications include microbial detection, antibacterial activity studies, and tumor microenvironment research.

NIAD-4 is a blood-brain barrier permeable fluorophore for optical imaging of amyloid-β (Aβ) in the central nervous system (CNS) for Alzheimer’s disease (AD). NIAD-4 binds to the same Aβ site with the binding affinity (K_i) of 10 nM.

1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity, can be used for RNA structure analysis.

Rhobo6 is a cell-impermeable glycan-binding, fluorescence turn-on imaging agent with a K_d of 53 µM for glycans. Rhobo6 reversibly binds target glycans and enables wash-free live ECM visualization. Rhobo6 can be used for fluorescent labeling of ECM in living samples or decellularized tissues (Ex/Em = 488/561 nm).

CDr17 is a GLUT1 substrate and selective fluorescent dye staining M1 microphages. CDr17 utilizes the Gating-Oriented Live-cell Distinction (GOLD) mechanism to enter M1 macrophages (Ex/Em = 646/662 nm).

Sulfobromophthalein (Bromosulfophthalein) disodium salt is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of agents and metabolites.

DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe.

2-Aminoacridone is a widely used fluorophore (λ_exc=428 nm, λ_em=525 nm).

9-Anthryldiazomethane is a fluorescent labeling reagent, which can be used for detecting fatty acids and derivatives.

Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.

DUPA-FITC is a fluorescent reagent targeting PSMA, which specifically binds to prostate cancer cells expressing PSMA without non-specific binding to normal blood cells. DUPA-FITC can label PSMA-expressing prostate cancer cells in whole blood, followed by internalization and trafficking to acidic intracellular endosomes, during which the fluorescence is quenched. When combined with flow cytometry and density gradient centrifugation enrichment, DUPA-FITC enables quantitative analysis of circulating tumor cells in peripheral blood samples from prostate cancer patients.