TPh–PyBz–β–gal

Guidelines
(The following is a recommended experimental protocol for guidance only, and needs to be adjusted according to your specific requirements)^[1].

1. Working Solution Preparation
1.2 Concentration recommendation: 10 mM.

2. Staining Procedure
2.1 Sample type: Adherent cells (HepG2 cells)
2.1.1 Adherent cells: No trypsin digestion is required before staining.
2.2 Incubation conditions: Incubate the 10 mM probe working solution with samples and β-gal in a humidified environment containing 5% CO₂ at 37°C for 35 min.
2.3 Washing step: Wash once with PBS.

3. Control Setup
3.1 Set up negative controls.
3.1.1 Negative control: Pre-treat cells with D-galactose (HY-N0210) first, then incubate the cells with the probe stock solution and β-gal together.

4. Detection and Analysis
4.1 Instrument: Laser confocal microscope.
4.1.1 Emission wavelength: 606 nm.
4.2 Result analysis:
4.2.1 Fluorescence intensity change: An activatable fluorescence signal is observable in the presence of β-gal; no obvious fluorescence is detected in cells treated with the probe alone or in pre-treated negative control cells.
4.2.3 Color change: A red fluorescence is emitted after recognition by β-gal.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.