905306-19-6
Chemical Structure
di-SFA
- CAS No.: 905306-19-6
- Formula:C45H35NNa2O10S2
- Molecular Weight:859.87
InChIKey: MAEYZDVPOVWCPS-LATBEIPCSA-L
SMILES: CCN(CC)C(C=C1)=CC(O2)=C1C=C2C(OC3=CC=C4/C=C/C5=CC(/C=C/C6=CC=C(C=C6)S(=O)(O[Na])=O)=CC(/C=C/C(C=C7)=CC=C7S(=O)(O[Na])=O)=C5)=C(C(C3=C4)=O)O
Biological Activity: di-SFA is a fluorescent probe used to measure transmembrane potential (Vm) across the plasma membrane. di-SFA contains a 3-hydroxychromone fluorophore that undergoes excited-state intramolecular proton transfer (ESIPT), producing dual emission from the normal (N*) and tautomeric (T*) states. di-SFA inserts deeply into the hydrophobic region of lipid bilayers. The sulfonate groups give di-SFA a double negative charge in aqueous media. Ratiometric measurements are performed in lipid vesicles using Ex/Em = 450/527 nm and Ex/Em = 450/605 nm.
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di-SFA | di-SFA is a fluorescent probe used to measure transmembrane potential (Vm) across the plasma membrane. di-SFA contains a 3-hydroxychromone fluorophore that undergoes excited-state intramolecular proton transfer (ESIPT), producing dual emission from the normal (N*) and tautomeric (T*) states. di-SFA inserts deeply into the hydrophobic region of lipid bilayers. The sulfonate groups give di-SFA a double negative charge in aqueous media. Ratiometric measurements are performed in lipid vesicles using Ex/Em = 450/527 nm and Ex/Em = 450/605 nm. | |||||||||||||||||||||
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