4-(Trifluoromethyl)umbelliferone

Cat. No.: HY-D0149 Purity: 98.69%: Data Sheet
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4-(Trifluoromethyl)umbelliferone is fluorescent probe substrate for rat hepatic cytochrome P450 enzymes.

For research use only. We do not sell to patients.

4-(Trifluoromethyl)umbelliferone Chemical Structure

CAS No. : 575-03-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

Biological Activity
Purity & Documentation
References
Customer Review

Description

4-(Trifluoromethyl)umbelliferone is fluorescent probe substrate for rat hepatic cytochrome P450 enzymes^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	0.19 3 Compound: 3c	Inhibition of human 17beta-HSD3 expressed in HeLa cells Inhibition of human 17beta-HSD3 expressed in HeLa cells	[PMID: 19954971]
SK-MEL-28	IC₅₀	1.1 3 Compound: 10	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
HeLa	IC₅₀	0.19 3 Compound: 3c	Inhibition of human 17beta-HSD3 expressed in HeLa cells Inhibition of human 17beta-HSD3 expressed in HeLa cells	[PMID: 19954971]
SK-MEL-28	IC₅₀	1.1 3 Compound: 10	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL-28	IC₅₀	1.1 3 Compound: 10	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	0.58 3 Compound: 10	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	0.58 3 Compound: 10	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	0.58 3 Compound: 10	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]

Molecular Weight

230.14

Formula

C₁₀H₅F₃O₃

CAS No.

575-03-1

Appearance

Solid

Color

Pale purple to light pink

Emission (Em)

460

Excitation (Ex)

335

SMILES

O=C1C=C(C(F)(F)F)C2=CC=C(O)C=C2O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (434.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3452 mL	21.7259 mL	43.4518 mL
5 mM	0.8690 mL	4.3452 mL	8.6904 mL
10 mM	0.4345 mL	2.1726 mL	4.3452 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.69%

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Data Sheet (270 KB) SDS (480 KB)

COA (250 KB) HNMR (130 KB) RP-HPLC (233 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Renwick AB, et, al. Evaluation of 7-benzyloxy-4-trifluoromethylcoumarin, some other 7-hydroxy-4-trifluoromethylcoumarin derivatives and 7-benzyloxyquinoline as fluorescent substrates for rat hepatic cytochrome P450 enzymes. Xenobiotica. 2001 Dec;31(12):861-78. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3452 mL	21.7259 mL	43.4518 mL	108.6295 mL
	5 mM	0.8690 mL	4.3452 mL	8.6904 mL	21.7259 mL
	10 mM	0.4345 mL	2.1726 mL	4.3452 mL	10.8630 mL
	15 mM	0.2897 mL	1.4484 mL	2.8968 mL	7.2420 mL
	20 mM	0.2173 mL	1.0863 mL	2.1726 mL	5.4315 mL
	25 mM	0.1738 mL	0.8690 mL	1.7381 mL	4.3452 mL
	30 mM	0.1448 mL	0.7242 mL	1.4484 mL	3.6210 mL
	40 mM	0.1086 mL	0.5431 mL	1.0863 mL	2.7157 mL
	50 mM	0.0869 mL	0.4345 mL	0.8690 mL	2.1726 mL
	60 mM	0.0724 mL	0.3621 mL	0.7242 mL	1.8105 mL
	80 mM	0.0543 mL	0.2716 mL	0.5431 mL	1.3579 mL
	100 mM	0.0435 mL	0.2173 mL	0.4345 mL	1.0863 mL