2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Methacholine bromide

Methacholine bromide  (Synonyms: Acetyl-β-methylcholine bromide)

Cat. No.: HY-A0083B
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Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

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No. CAS : 333-31-3

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Methacholine bromide Related Antibodies

Top Publications Citing Use of Products

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716.  [Abstract]

    Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

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    Descripciòn

    Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates[1][2][3][4].

    In Vivo

    Methacholine bromide (0.5 µg/kg plus 5 µg/kg/min for 30 min) induces bronchoconstriction in dogs[4].
    Methacholine bromide (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 µg/kg; i.v.) in a a dose-dependent manner in mouse[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 9-week female BALB/c mice[6]
    Dosage: 0.03, 0.1, 0.3, 1 mg/kg
    Administration: I.v
    Result: Induced severe bronchoconstriction.
    Peso molecular

    240.14

    Fòrmula

    C8H18BrNO2
    No. CAS
    SMILES

    CC(OC(C)=O)C[N+](C)(C)C.[Br-]
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    Please store the product under the recommended conditions in the Certificate of Analysis.
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    Methacholine bromide Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methacholine333-31-3Acetyl-β-methylcholinemAChRMuscarinic acetylcholine receptorbronchoconstrictionairway hyperresponsivenessasthma-likeInhibitorinhibitorinhibit

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