2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Methacholine iodide

Methacholine iodide  (Synonyms: Acetyl-β-methylcholine iodide)

Cat. No.: HY-A0083C
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Methacholine iodide is a potent muscarinic-3 (M3) agonist. Methacholine iodide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine iodide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine iodide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

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CAS No. : 625-19-4

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Methacholine iodide Related Antibodies

Top Publications Citing Use of Products

    Methacholine iodide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716.  [Abstract]

    Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.

    Methacholine iodide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.

    Methacholine iodide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine iodide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine iodide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

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    Description

    Methacholine iodide is a potent muscarinic-3 (M3) agonist. Methacholine iodide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine iodide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine iodide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates[1][2][3][4].

    Molecular Weight

    287.14

    Formula

    C8H18INO2
    CAS No.
    SMILES

    O=C(C)OC(C[N+](C)(C)C)C.[I-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Methacholine iodide Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methacholine625-19-4Acetyl-β-methylcholinemAChRMuscarinic acetylcholine receptorbronchoconstrictionairway hyperresponsivenessasthma-likeInhibitorinhibitorinhibit

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