2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Methacholine bromide

Methacholine bromide  (Synonyms: Acetyl-β-methylcholine bromide)

Cat. No.: HY-A0083B
Handling Instructions Technical Support

Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

For research use only. We do not sell to patients.

CAS No. : 333-31-3

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Methacholine bromide:

Other Forms of Methacholine bromide:

Methacholine bromide Related Antibodies

Top Publications Citing Use of Products

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716.  [Abstract]

    Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125.  [Abstract]

    AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

    View All mAChR Isoform Specific Products:

    View All Isoforms
    mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates[1][2][3][4].

    In Vivo

    Methacholine bromide (0.5 µg/kg plus 5 µg/kg/min for 30 min) induces bronchoconstriction in dogs[4].
    Methacholine bromide (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 µg/kg; i.v.) in a a dose-dependent manner in mouse[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 9-week female BALB/c mice[6]
    Dosage: 0.03, 0.1, 0.3, 1 mg/kg
    Administration: I.v
    Result: Induced severe bronchoconstriction.
    Molecular Weight

    240.14

    Formula

    C8H18BrNO2
    CAS No.
    SMILES

    CC(OC(C)=O)C[N+](C)(C)C.[Br-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Methacholine bromide Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methacholine333-31-3Acetyl-β-methylcholinemAChRMuscarinic acetylcholine receptorbronchoconstrictionairway hyperresponsivenessasthma-likeInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Methacholine bromide
    Cat. No.:
    HY-A0083B
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.