2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Methacholine chloride

Methacholine chloride  (Synonyms: Acetyl-β-methylcholine chloride)

Cat. No.: HY-A0083 Purity: ≥98.0%
COA Handling Instructions

Methacholine (Acetyl-β-methylcholine) choride is a potent muscarinic-3 (M3) agonist. Methacholine choride acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine choride shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine choride can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

For research use only. We do not sell to patients.

Methacholine chloride Chemical Structure

Methacholine chloride Chemical Structure

CAS No. : 62-51-1

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Solution
10 mM * 1 mL in DMSO USD 79 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Methacholine chloride:

Methacholine chloride Related Antibodies

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1 Publications Citing Use of MCE Methacholine chloride

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Description

Methacholine (Acetyl-β-methylcholine) choride is a potent muscarinic-3 (M3) agonist. Methacholine choride acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine choride shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine choride can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates[1][2][3][4].

In Vivo

Methacholine choride (0.5 µg/kg plus 5 µg/kg/min for 30 min) induces bronchoconstriction in dogs[4].
Methacholine choride (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 µg/kg; i.v.) in a a dose-dependent manner in mouse[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 9-week female BALB/c mice[6]
Dosage: 0.03, 0.1, 0.3, 1 mg/kg
Administration: I.v.
Result: Induced severe bronchoconstriction.
Clinical Trial
Molecular Weight

195.69

Appearance

Solid

Formula

C8H18ClNO2
CAS No.
SMILES

CC(OC(C)=O)C[N+](C)(C)C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (511.01 mM; Need ultrasonic)

H2O : 100 mg/mL (511.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.1101 mL 25.5506 mL 51.1012 mL
5 mM 1.0220 mL 5.1101 mL 10.2202 mL
10 mM 0.5110 mL 2.5551 mL 5.1101 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 130 mg/mL (664.32 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: ≥98.0%

COA (245 KB) HNMR (249 KB)

Handling Instructions (2659 KB)
References
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Methacholine chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Keywords:

Methacholine62-51-1Acetyl-β-methylcholinemAChRMuscarinic acetylcholine receptorbronchoconstrictionairway hyperresponsivenessasthma-likeInhibitorinhibitorinhibit

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