Methacholine chloride (Synonyms: Acetyl-β-methylcholine chloride)

Name: Methacholine chloride
Brand: MedChemExpress
SKU: HY-A0083
Rating: 5.0 (6 reviews)

Cat. No.: HY-A0083 Purity: 98.0%: Data Sheet
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Methacholine (Acetyl-β-methylcholine) choride is a potent muscarinic-3 (M3) agonist. Methacholine choride acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine choride shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine choride can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

For research use only. We do not sell to patients.

CAS No. : 62-51-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 6 publication(s) in Google Scholar

Other Forms of Methacholine chloride:

Methacholine bromide Get quote
Methacholine iodide Get quote
Methacholine chloride (Standard) Get quote

6 Publications Citing Use of MCE Methacholine chloride

In Vivo Efficacy Study

: Methacholine chloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716. [Abstract]; Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.

: Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]; AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.

: Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]; AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

: Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]; AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

: Methacholine chloride purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]; AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

mAChR3

In Vivo

Methacholine choride (0.5 μg/kg plus 5 μg/kg/min for 30 min) induces bronchoconstriction in dogs^[4].
Methacholine choride (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 μg/kg; i.v.) in a a dose-dependent manner in mouse^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	9-week female BALB/c mice^[6]
Dosage:	0.03, 0.1, 0.3, 1 mg/kg
Administration:	I.v.
Result:	Induced severe bronchoconstriction.

Clinical Trial

Molecular Weight

195.69

Formula

C₈H₁₈ClNO₂

CAS No.

62-51-1

Appearance

Solid

Color

White to off-white

SMILES

CC(OC(C)=O)C[N+](C)(C)C.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (511.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (511.01 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1101 mL	25.5506 mL	51.1012 mL
5 mM	1.0220 mL	5.1101 mL	10.2202 mL
10 mM	0.5110 mL	2.5551 mL	5.1101 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 130 mg/mL (664.32 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (274 KB) SDS (396 KB)

COA (246 KB) HNMR (236 KB)

Handling Instructions (2659 KB)

References

[1]. Cohen J, et al. Relationship between airway responsiveness to neurokinin A and methacholine in asthma. Pulm Pharmacol Ther. 2005;18(3):171-176. [Content Brief]

[2]. Anderson SD, et al. Comparison of mannitol and methacholine to predict exercise-induced bronchoconstriction and a clinical diagnosis of asthma. Respir Res. 2009;10(1):4. Published 2009 Jan 23. [Content Brief]

[3]. Cockcroft DW. Methacholine challenge methods. Chest. 2008;134(4):678-680. [Content Brief]

[4]. Kabara S, et al. Differential effects of thiopental on methacholine- and serotonin-induced bronchoconstriction in dogs. Br J Anaesth. 2003 Sep;91(3):379-84. [Content Brief]

[5]. Folkerts G, et al. Bradykinin causes inhibition of methacholine-induced bronchoconstriction in vivo in mice. Naunyn Schmiedebergs Arch Pharmacol. 2001 Jul;364(1):53-8. [Content Brief]

[6]. Vitorasso RL, et al. Methacholine dose response curve and acceptability criteria of respiratory mechanics modeling. Exp Lung Res. 2020 Feb-Mar;46(1-2):23-31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	5.1101 mL	25.5506 mL	51.1012 mL	127.7531 mL
	5 mM	1.0220 mL	5.1101 mL	10.2202 mL	25.5506 mL
	10 mM	0.5110 mL	2.5551 mL	5.1101 mL	12.7753 mL
	15 mM	0.3407 mL	1.7034 mL	3.4067 mL	8.5169 mL
	20 mM	0.2555 mL	1.2775 mL	2.5551 mL	6.3877 mL
	25 mM	0.2044 mL	1.0220 mL	2.0440 mL	5.1101 mL
	30 mM	0.1703 mL	0.8517 mL	1.7034 mL	4.2584 mL
	40 mM	0.1278 mL	0.6388 mL	1.2775 mL	3.1938 mL
	50 mM	0.1022 mL	0.5110 mL	1.0220 mL	2.5551 mL
	60 mM	0.0852 mL	0.4258 mL	0.8517 mL	2.1292 mL
	80 mM	0.0639 mL	0.3194 mL	0.6388 mL	1.5969 mL
	100 mM	0.0511 mL	0.2555 mL	0.5110 mL	1.2775 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Methacholine chloride Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methacholine62-51-1Acetyl-β-methylcholinemAChRMuscarinic acetylcholine receptorbronchoconstrictionairway hyperresponsivenessasthma-likeInhibitorinhibitorinhibit

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Methacholine chloride (Synonyms: Acetyl-β-methylcholine chloride)

Customer Review

Other Forms of Methacholine chloride:

Methacholine chloride Related Antibodies

6 Publications Citing Use of MCE Methacholine chloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Methacholine chloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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