ML133 (hydrochloride)

Name: ML133 hydrochloride
Brand: MedChemExpress
SKU: HY-100230A
Rating: 5.0 (2 reviews)

製品番号: HY-100230A 純度: 99.82%: Data Sheet SDS COA 取扱説明書
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ML133 hydrochloride is a selective K_ir2 family channels inhibitor, with an IC₅₀ of 1.8 μM at pH 7.4 and 290 nM at pH 8.5.

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ML133 (hydrochloride) 構造式

CAS 番号 : 1222781-70-5

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 60	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 60	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$55	在庫あり	Estimated Time of Arrival: December 31
10 mg	$77	在庫あり	Estimated Time of Arrival: December 31
25 mg	$150	在庫あり	Estimated Time of Arrival: December 31
50 mg	$231	在庫あり	Estimated Time of Arrival: December 31
100 mg	$385	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of ML133 hydrochloride:

ML-133 お問い合わせ
ML133 hydrochloride (Standard) お問い合わせ

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純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

ML133 hydrochloride is a selective K_ir2 family channels inhibitor, with an IC₅₀ of 1.8 μM at pH 7.4 and 290 nM at pH 8.5^[1].

IC₅₀ & Target

IC50: 1.8 μM (K_ir2 at pH 7.4), 290 nM (K_ir2 at pH 8.5)^[1].

体外実験

ML133 hydrochloride, which inhibits K_ir2.1 with IC₅₀ of 1.8 μM at pH 7.4 and 290 nM at pH 8.5, but exhibits little selectivity against other members of K_ir2.x family channels^[1].
ML133 hydrochloride has no effect on K_ir1.1 (IC₅₀ > 300 μM), and displays weak activity for K_ir4.1 (76 μM) and K_ir7.1 (33 μM), making ML133 the most selective small molecule inhibitor of the Kir family reported to date^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

313.82

分子式

C₁₉H₂₀ClNO

CAS 番号

1222781-70-5

Appearance

Solid

Color

White to off-white

SMILES

COC1=CC=C(CNCC2=C3C=CC=CC3=CC=C2)C=C1.[H]Cl

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶剤 & 溶解度

体外:

DMSO : 87.5 mg/mL (278.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1865 mL	15.9327 mL	31.8654 mL
5 mM	0.6373 mL	3.1865 mL	6.3731 mL
10 mM	0.3187 mL	1.5933 mL	3.1865 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.82%

Select Batch:

データシート (275 KB) SDS (417 KB)

COA (251 KB) HNMR (268 KB) LCMS (87 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Wang HR, et al. Selective inhibition of the K(ir)2 family of inward rectifier potassium channels by a small molecule probe: the discovery, SAR, and pharmacological characterization of ML133. ACS Chem Biol. 2011 Aug 19;6(8):845-56. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1865 mL	15.9327 mL	31.8654 mL	79.6635 mL
	5 mM	0.6373 mL	3.1865 mL	6.3731 mL	15.9327 mL
	10 mM	0.3187 mL	1.5933 mL	3.1865 mL	7.9663 mL
	15 mM	0.2124 mL	1.0622 mL	2.1244 mL	5.3109 mL
	20 mM	0.1593 mL	0.7966 mL	1.5933 mL	3.9832 mL
	25 mM	0.1275 mL	0.6373 mL	1.2746 mL	3.1865 mL
	30 mM	0.1062 mL	0.5311 mL	1.0622 mL	2.6554 mL
	40 mM	0.0797 mL	0.3983 mL	0.7966 mL	1.9916 mL
	50 mM	0.0637 mL	0.3187 mL	0.6373 mL	1.5933 mL
	60 mM	0.0531 mL	0.2655 mL	0.5311 mL	1.3277 mL
	80 mM	0.0398 mL	0.1992 mL	0.3983 mL	0.9958 mL
	100 mM	0.0319 mL	0.1593 mL	0.3187 mL	0.7966 mL