Necrostatin 2 (S enantiomer)

Name: Necrostatin 2 (S enantiomer)
Brand: MedChemExpress
SKU: HY-14622B
Rating: 4.5 (0 reviews)

製品番号: HY-14622B 純度: 99.76%: Data Sheet SDS COA 取扱説明書
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Necrostatin 2 S enantiomer is the S enantiomer of Necrostatin 2 (HY-14622). Necrostatin 2 S enantiomer inhibits TNF-α-induced necroptosis in Jurkat T cells. Necrostatin 2 S enantiomer can be used for the research of ischemic brain injury (stroke).

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Necrostatin 2 (S enantiomer) 構造式

CAS 番号 : 852391-20-9

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 209	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 209	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$190	在庫あり	Estimated Time of Arrival: December 31
10 mg	$310	在庫あり	Estimated Time of Arrival: December 31
25 mg	$495	在庫あり	Estimated Time of Arrival: December 31
50 mg	$695	在庫あり	Estimated Time of Arrival: December 31
100 mg		お問い合わせ
200 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of Necrostatin 2 (S enantiomer):

Necrostatin 2 在庫あり
Necrostatin 2 racemate 在庫あり

RIP kinase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

RIPK1 RIPK2 RIPK3

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target^[1]

RIPK1

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	EC₅₀	0.23 μM Compound: 26	Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha	[PMID: 16153840]

体外実験

Necrostatin 2 S enantiomer (24 h) inhibits TNF-α-induced necroptosis in FADD-deficient human Jurkat T cells with an EC₅₀ of 0.23 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

277.71

分子式

C₁₃H₁₂ClN₃O₂

CAS 番号

852391-20-9

Appearance

Solid

Color

White to light yellow

SMILES

O=C1N(C)C([C@H](CC2=CNC3=C2C=CC=C3Cl)N1)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (180.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6009 mL	18.0044 mL	36.0088 mL
5 mM	0.7202 mL	3.6009 mL	7.2018 mL
10 mM	0.3601 mL	1.8004 mL	3.6009 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.76%

Select Batch:

データシート (277 KB) SDS (251 KB)

COA (255 KB) LCMS (119 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Teng X, et al. Structure-activity relationship study of novel necroptosis inhibitors. Bioorg Med Chem Lett. 2005;15(22):5039-5044. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6009 mL	18.0044 mL	36.0088 mL	90.0220 mL
	5 mM	0.7202 mL	3.6009 mL	7.2018 mL	18.0044 mL
	10 mM	0.3601 mL	1.8004 mL	3.6009 mL	9.0022 mL
	15 mM	0.2401 mL	1.2003 mL	2.4006 mL	6.0015 mL
	20 mM	0.1800 mL	0.9002 mL	1.8004 mL	4.5011 mL
	25 mM	0.1440 mL	0.7202 mL	1.4404 mL	3.6009 mL
	30 mM	0.1200 mL	0.6001 mL	1.2003 mL	3.0007 mL
	40 mM	0.0900 mL	0.4501 mL	0.9002 mL	2.2505 mL
	50 mM	0.0720 mL	0.3601 mL	0.7202 mL	1.8004 mL
	60 mM	0.0600 mL	0.3001 mL	0.6001 mL	1.5004 mL
	80 mM	0.0450 mL	0.2251 mL	0.4501 mL	1.1253 mL
	100 mM	0.0360 mL	0.1800 mL	0.3601 mL	0.9002 mL