Cat. No.: HY-14622A

Necrostatin 2 racemate

(Synonyms: Necrostatin 1S; Nec-1S; 7-Cl-O-Nec1)

34 Cited Publications

Customer Review

Based on 34 publication(s) in Google Scholar

Necrostatin 2 racemate (Nec-1S), the Necrostatin-1 (HY-15760) stable, is a potent and specific RIPK1 inhibitor lacking the IDO-targeting effect.

For research use only. We do not sell to patients.

Purity: 99.57%
CAS No.: 852391-15-2
Formula: C₁₃H₁₂ClN₃O₂
Molecular Weight:277.71

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Necrostatin 2 racemate Chemical Structure

CAS No.: 852391-15-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (34)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Necrostatin 2 racemate (Nec-1S), the Necrostatin-1 (HY-15760) stable, is a potent and specific RIPK1 inhibitor lacking the IDO-targeting effect^[1].

IC₅₀ & Target^[1]

RIPK1

All RIP kinase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	EC₅₀	0.21 μM Compound: 66	Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha	[PMID: 16153840]
Jurkat	IC₅₀	206 nM Compound: 4, 7-cl-O-Nec-1	Inhibition of death receptor signaling mediated necroptotic cell death in human Jurkat cell deficient in FADD assessed as cell viability after 30 hrs by ATP based viability assay in presence of TNFalpha Inhibition of death receptor signaling mediated necroptotic cell death in human Jurkat cell deficient in FADD assessed as cell viability after 30 hrs by ATP based viability assay in presence of TNFalpha	[PMID: 16408008]

In Vitro

Necrostatin 2 racemate (Nec-1S) (1-100 μM; 1 h) potently inhibits RIPK1 autophosphorylation in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Necrostatin 2 racemate (Nec-1S) (6 mg/kg; i.v.; once) protects against TNF-induced systemic inflammatory response syndrome (SIRS) without sensitization effect^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female C57BL/6J WT mice, SIRS model^[1]
Dosage:

0.6 mg/kg or 6 mg/kg
Administration:

Intravenous injection, once
Result:

Showed protective effect at 6 mg/kg.

Chemical Information

CAS No. 852391-15-2
Appearance Solid
Molecular Weight 277.71
Formula C₁₃H₁₂ClN₃O₂
Color White to light yellow
SMILES

ClC1=C2C(C(CC3C(N(C)C(N3)=O)=O)=CN2)=CC=C1
Synonyms

Necrostatin 1S; Nec-1S; 7-Cl-O-Nec1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (34)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyWBIP

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Journal Impact Factor

Adv Mater

Dismantling the Necroptotic Engine: An Oral Theranostic Nanosponge for Ulcerative Colitis. [Abstract]

2026 Jan 16:e16297. PMID: 41546396
Cell Stem Cell

Bacterial ADP-heptose triggers stem cell regeneration in the intestinal epithelium following injury. [Abstract]

2025 Aug 7;32(8):1235-1250.e6. PMID: 40651470
Autophagy

Kitasamycin overcomes ferroptosis and immunotherapy resistance by targeting the HUWE1-NCOA4-FTH1 axis. [Abstract]

2026 Feb 15:1-19. PMID: 41612599
Nat Commun

Intrinsic temperature increase drives lipid metabolism towards ferroptosis evasion and chemotherapy resistance in pancreatic cancer. [Abstract]

2024 Oct 2;15(1):8540. PMID: 39358362

Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 2;15(1):8540. [Abstract]

Necrostatin 2 racemate (Nec-1s; 20 µM; 500 h). Time-course confluence of PANC-1 or BxPC3 cells treated with GEM and supplemented with Nec-1s, Fer-1 or ZVAD.
Nat Commun

SETBP1 accumulation induces P53 inhibition and genotoxic stress in neural progenitors underlying neurodegeneration in Schinzel-Giedion syndrome. [Abstract]

2021 Jun 30;12(1):4050. PMID: 34193871
Redox Biol

SLC7A11 protects luminal A breast cancer cells against ferroptosis induced by CDK4/6 inhibitors. [Abstract]

2024 Oct:76:103304. PMID: 39153252

Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Oct:76:103304. [Abstract]

Cell viability in T-47D and MCF-7 cells lines treated with 1.5 μM Abemaciclib in the absence or presence of 2 μM Necrostatin-1s for 24 h.
Adv Sci (Weinh)

Development of a New Positron Emission Tomography Imaging Radioligand Targeting RIPK1 in the Brain and Characterization in Alzheimer's Disease. [Abstract]

2024 Aug;11(32):e2309021. PMID: 38923244

Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Aug;11(32):e2309021. [Abstract]

Time-activity curves of baseline and self-blocking (pretreatment with unlabeled Nec-1s 1.0 and 3.0 mg/kg, i.v.).
Cell Rep Med

Vorapaxar enhanced mitochondria-associated ferroptosis primes cancer immunotherapy via targeting FOXO1/HMOX1 axis. [Abstract]

2025 Sep 25:102371. PMID: 41005298
Cell Death Differ

Cell death-induced release of the pro-aging protein acyl CoA binding protein (ACBP) into the circulation. [Abstract]

2026 Jun 3. PMID: 42236908
Cell Death Differ

RIPK3 sequentially recruits MLKL and RIPK1 to induce PANoptosis and chemokine production. [Abstract]

2026 Apr 4. PMID: 41935203
Cell Death Dis

IFI16 is essential to linking DNA damage and ferroptosis in acute kidney injury. [Abstract]

2026 Mar 23;17(1):350. PMID: 41872158
Cell Death Dis

Programmed cell death pathways coordinate neutrophil and macrophage clearance in zebrafish and are differentially exploited by Salmonella Typhimurium. [Abstract]

2025 Dec 8. PMID: 41360763
Cell Death Dis

Peroxisomal membrane protein PMP70 confers drug resistance in colorectal cancer. [Abstract]

2025 Apr 14;16(1):293. PMID: 40229252
Cell Death Dis

LINC01133 can induce acquired ferroptosis resistance by enhancing the FSP1 mRNA stability through forming the LINC01133-FUS-FSP1 complex. [Abstract]

2023 Nov 25;14(11):767. PMID: 38007473
Cell Death Dis

TRAF6 regulates the abundance of RIPK1 and inhibits the RIPK1/RIPK3/MLKL necroptosis signaling pathway and affects the progression of colorectal cancer. [Abstract]

2023 Jan 5;14(1):6. PMID: 36604411
J Immunother Cancer

Propafenone facilitates mitochondrial-associated ferroptosis and synergizes with immunotherapy in melanoma. [Abstract]

2024 Nov 24;12(11):e009805. PMID: 39581704
J Exp Med

Adenosine metabolic clearance maintains liver homeostasis by licensing arginine methylation of RIPK1. [Abstract]

2026 Jan 5;223(1):e20250603. PMID: 41081715

Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Jan 5;223(1):e20250603. [Abstract]

Primary hepatocytes from Adk^f/f or Adk^f/f; Alb-Cre mice were treated with CHX (1 μM)/ TNFα (10 ng/ml) for 12 h in the presence or absence of Nec-1s (10 μM).

Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Jan 5;223(1):e20250603. [Abstract]

HEK293T cells were transfected with expression vectors for Flag-RIPK1 WT or Flag-RIPK1-R606K in the presence or absence of Nec-1s (10 μM), respectively. The autophosphorylation of RIPK1 was determined with immunoblotting.
J Cachexia Sarcopenia Muscle

Amelioration of inflammatory myopathies by glucagon-like peptide-1 receptor agonist via suppressing muscle fibre necroptosis. [Abstract]

2022 Aug;13(4):2118-2131. PMID: 35775116
Free Radic Biol Med

Dehydroascorbic acid impairs neurite growth through RIPK1-associated caspase activation. [Abstract]

2025 Nov:239:406-416. PMID: 40730291
Cell Rep

ISG15 negatively regulates RIPK3-mediated cell death and viral pathogenesis. [Abstract]

2026 May 26;45(5):117360. PMID: 42139056
Blood Adv

Double-negative T cells utilize a TNFα-JAK1-ICAM-1 cytotoxic axis against acute myeloid leukemia. [Abstract]

2024 Jun 25;8(12):3013-3026. PMID: 38547431
Mol Neurobiol

3-n-Butylphthalide Protects SH-SY5Y Cells from Ferroptosis by Inhibiting ACSL4-Mediated Lipid Peroxidation. [Abstract]

2026 Jan 16;63(1):369. PMID: 41545774
Biol Direct

MINPP1 promotes ferroptosis in HBV-related hepatocellular carcinoma by regulating CTSB K33-linked deubiquitination via ZRANB1. [Abstract]

2025 Oct 1;20(1):100. PMID: 41035046
J Lipid Res

Hyperactivation of SREBP induces Pannexin-1-dependent lytic cell death. [Abstract]

2024 Jul;65(7):100579. PMID: 38880128
FASEB J

Renal tubular epithelial cell necroptosis promotes tubulointerstitial fibrosis in patients with chronic kidney disease. [Abstract]

2022 Dec;36(12):e22625. PMID: 36331546
J Med Virol

HPV16 Enhances Radiosensitivity in Esophageal Squamous Cell Carcinoma by Promoting De Novo Fatty Acid Synthesis and Disulfidptosis. [Abstract]

2025 Nov;97(11):e70699. PMID: 41231234
University of Toronto

2025.
Res Sq

2025 Sep 18.
bioRxiv

2025 Sep 5.
University of Toronto

2025.
bioRxiv

2025 January 27.
bioRxiv

TBK1 and IKKε protect target cells from IFNγ-mediated T cell killing via an inflammatory apoptotic mechanism. [Abstract]

2024 Aug 8:2024.08.06.606693. PMID: 39149268
bioRxiv

2023 Oct 2.
Research Square Print

November 8th, 2022

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (180.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6009 mL

18.0044 mL

36.0088 mL

5 mM

0.7202 mL

3.6009 mL

7.2018 mL

10 mM

0.3601 mL

1.8004 mL

3.6009 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Takahashi N, et al. Necrostatin-1 analogues: critical issues on the specificity, activity and in vivo use in experimental disease models. Cell Death Dis. 2012 Nov 29;3:e437. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6009 mL	18.0044 mL	36.0088 mL	90.0220 mL
	5 mM	0.7202 mL	3.6009 mL	7.2018 mL	18.0044 mL
	10 mM	0.3601 mL	1.8004 mL	3.6009 mL	9.0022 mL
	15 mM	0.2401 mL	1.2003 mL	2.4006 mL	6.0015 mL
	20 mM	0.1800 mL	0.9002 mL	1.8004 mL	4.5011 mL
	25 mM	0.1440 mL	0.7202 mL	1.4404 mL	3.6009 mL
	30 mM	0.1200 mL	0.6001 mL	1.2003 mL	3.0007 mL
	40 mM	0.0900 mL	0.4501 mL	0.9002 mL	2.2505 mL
	50 mM	0.0720 mL	0.3601 mL	0.7202 mL	1.8004 mL
	60 mM	0.0600 mL	0.3001 mL	0.6001 mL	1.5004 mL
	80 mM	0.0450 mL	0.2251 mL	0.4501 mL	1.1253 mL
	100 mM	0.0360 mL	0.1800 mL	0.3601 mL	0.9002 mL

Keywords

Necrostatin 2 racemate

34 Cited Publications

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Necrostatin 2 racemate

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

Covalent Screening Library

Human Metabolite Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

MCE Bioactive Compound Library

Kinase Inhibitor Library Mini

Covalent Screening Library Plus

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Posttranslational Modification Library

3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) Necrostatin 2 racemate

All RIP kinase Isoforms

Biological Activity

Chemical Information

Publications (34)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Necrostatin 2 racemate

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

Covalent Screening Library

Human Metabolite Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

MCE Bioactive Compound Library

Kinase Inhibitor Library Mini

Covalent Screening Library Plus

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Posttranslational Modification Library

3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) Necrostatin 2 racemate

All RIP kinase Isoforms

Biological Activity

Chemical Information

Publications (34)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Necrostatin 2 racemate Related Classifications

HY-14622A Related Classifications