Necrostatin 2 racemate (Synonyms: Necrostatin 1S; Nec-1S; 7-Cl-O-Nec1)

Name: Necrostatin 2 racemate
Brand: MedChemExpress
SKU: HY-14622A
Rating: 5.0 (30 reviews)

Cat. No.: HY-14622A Purity: 99.57%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Necrostatin 2 racemate (Nec-1S), the Necrostatin-1 (HY-15760) stable, is a potent and specific RIPK1 inhibitor lacking the IDO-targeting effect.

For research use only. We do not sell to patients.

CAS No. : 852391-15-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 30 publication(s) in Google Scholar

Other Forms of Necrostatin 2 racemate:

Necrostatin 2 In-stock
Necrostatin 2 S enantiomer In-stock
Necrostatin 2 racemate (Standard) Get quote

30 Publications Citing Use of MCE Necrostatin 2 racemate

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

: Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Jan 5;223(1):e20250603. [Abstract]; Primary hepatocytes from Adk^f/f or Adk^f/f; Alb-Cre mice were treated with CHX (1 μM)/ TNFα (10 ng/ml) for 12 h in the presence or absence of Nec-1s (10 μM).

: Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Jan 5;223(1):e20250603. [Abstract]; HEK293T cells were transfected with expression vectors for Flag-RIPK1 WT or Flag-RIPK1-R606K in the presence or absence of Nec-1s (10 μM), respectively. The autophosphorylation of RIPK1 was determined with immunoblotting.

: Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 2;15(1):8540. [Abstract]; Necrostatin 2 racemate (Nec-1s; 20 µM; 500 h). Time-course confluence of PANC-1 or BxPC3 cells treated with GEM and supplemented with Nec-1s, Fer-1 or ZVAD.

: Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 25:e2309021. [Abstract]; Time-activity curves of baseline and self-blocking (pretreatment with unlabeled Nec-1s 1.0 and 3.0 mg/kg, i.v.).

: Necrostatin 2 racemate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Aug 10:76:103304. [Abstract]; Cell viability in T-47D and MCF-7 cells lines treated with 1.5 μM Abemaciclib in the absence or presence of 2 μM Necrostatin-1s for 24 h.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Necrostatin 2 racemate (Nec-1S), the Necrostatin-1 (HY-15760) stable, is a potent and specific RIPK1 inhibitor lacking the IDO-targeting effect^[1].

IC₅₀ & Target^[1]

RIPK1

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	EC₅₀	0.21 μM Compound: 66	Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha	[PMID: 16153840]
Jurkat	IC₅₀	206 nM Compound: 4, 7-cl-O-Nec-1	Inhibition of death receptor signaling mediated necroptotic cell death in human Jurkat cell deficient in FADD assessed as cell viability after 30 hrs by ATP based viability assay in presence of TNFalpha Inhibition of death receptor signaling mediated necroptotic cell death in human Jurkat cell deficient in FADD assessed as cell viability after 30 hrs by ATP based viability assay in presence of TNFalpha	[PMID: 16408008]

In Vitro

Necrostatin 2 racemate (Nec-1S) (1-100 μM; 1 h) potently inhibits RIPK1 autophosphorylation in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Necrostatin 2 racemate (Nec-1S) (6 mg/kg; i.v.; once) protects against TNF-induced systemic inflammatory response syndrome (SIRS) without sensitization effect^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6J WT mice, SIRS model^[1]
Dosage:	0.6 mg/kg or 6 mg/kg
Administration:	Intravenous injection, once
Result:	Showed protective effect at 6 mg/kg.

Molecular Weight

277.71

Formula

C₁₃H₁₂ClN₃O₂

CAS No.

852391-15-2

Appearance

Solid

Color

White to light yellow

SMILES

ClC1=C2C(C(CC3C(N(C)C(N3)=O)=O)=CN2)=CC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (180.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6009 mL	18.0044 mL	36.0088 mL
5 mM	0.7202 mL	3.6009 mL	7.2018 mL
10 mM	0.3601 mL	1.8004 mL	3.6009 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (256 KB) HNMR (143 KB) RP-HPLC (261 KB) MS (73 KB)

Handling Instructions (2659 KB)

References

[1]. Takahashi N, et al. Necrostatin-1 analogues: critical issues on the specificity, activity and in vivo use in experimental disease models. Cell Death Dis. 2012 Nov 29;3:e437. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6009 mL	18.0044 mL	36.0088 mL	90.0220 mL
	5 mM	0.7202 mL	3.6009 mL	7.2018 mL	18.0044 mL
	10 mM	0.3601 mL	1.8004 mL	3.6009 mL	9.0022 mL
	15 mM	0.2401 mL	1.2003 mL	2.4006 mL	6.0015 mL
	20 mM	0.1800 mL	0.9002 mL	1.8004 mL	4.5011 mL
	25 mM	0.1440 mL	0.7202 mL	1.4404 mL	3.6009 mL
	30 mM	0.1200 mL	0.6001 mL	1.2003 mL	3.0007 mL
	40 mM	0.0900 mL	0.4501 mL	0.9002 mL	2.2505 mL
	50 mM	0.0720 mL	0.3601 mL	0.7202 mL	1.8004 mL
	60 mM	0.0600 mL	0.3001 mL	0.6001 mL	1.5004 mL
	80 mM	0.0450 mL	0.2251 mL	0.4501 mL	1.1253 mL
	100 mM	0.0360 mL	0.1800 mL	0.3601 mL	0.9002 mL

Necrostatin 2 racemate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Necrostatin 2852391-15-2Necrostatin 1S Nec-1S 7-Cl-O-Nec1Necrostatin2Necrostatin-2RIP kinaseReceptor-interacting protein kinasesRIPKNec-1stableRIPK1necroptosisIDOnecrostatinInhibitorinhibitorinhibit

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Necrostatin 2 racemate (Synonyms: Necrostatin 1S; Nec-1S; 7-Cl-O-Nec1)

Customer Review

Other Forms of Necrostatin 2 racemate:

Necrostatin 2 racemate Related Antibodies

30 Publications Citing Use of MCE Necrostatin 2 racemate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All RIP kinase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Necrostatin 2 racemate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Necrostatin 2 racemate Related Classifications

Keywords:

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Customer Review

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