Necrostatin 2 (S enantiomer)
Based on 1 Customer Validation
Necrostatin 2 S enantiomer is the S enantiomer of Necrostatin 2 (HY-14622). Necrostatin 2 S enantiomer inhibits TNF-α-induced necroptosis in Jurkat T cells. Necrostatin 2 S enantiomer can be used for the research of ischemic brain injury (stroke).
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 852391-20-9
- Formula: C13H12ClN3O2
- Molecular Weight:277.71
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-14622
Necrostatin 2
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HY-14622A
Necrostatin 2 racemate
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HY-A0003
Lenalidomide
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HY-15760
Necrostatin-1
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HY-10984
Pomalidomide
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HY-A0003R
Lenalidomide (Standard)
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HY-13743
Roquinimex
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HY-101872A
GSK-872 hydrochloride
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HY-111255
SPD304
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HY-18937
WEHI-345
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HY-15760R
Necrostatin-1 (Standard)
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HY-14622AR
Necrostatin 2 racemate (Standard)
Biological Activity
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RIPK1 |
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Cell Line
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Type | Value | Description | References |
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| Jurkat | EC50 |
0.23 μM
Compound: 26
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Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha
Effective concentration required for inhibition of necroptosis in FADD deficient Jurkat T cells treated with TNF-alpha
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[PMID: 16153840] |
Necrostatin 2 S enantiomer (24 h) inhibits TNF-α-induced necroptosis in FADD-deficient human Jurkat T cells with an EC50 of 0.23 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 852391-20-9
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Appearance Solid
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Molecular Weight 277.71
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Formula C13H12ClN3O2
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Color White to light yellow
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SMILES
O=C1N(C)C([C@H](CC2=CNC3=C2C=CC=C3Cl)N1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 50 mg/mL (180.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution
This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (10.80 mM); Clear solution
This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6009 mL | 18.0044 mL | 36.0088 mL | 90.0220 mL |
| 5 mM | 0.7202 mL | 3.6009 mL | 7.2018 mL | 18.0044 mL | |
| 10 mM | 0.3601 mL | 1.8004 mL | 3.6009 mL | 9.0022 mL | |
| 15 mM | 0.2401 mL | 1.2003 mL | 2.4006 mL | 6.0015 mL | |
| 20 mM | 0.1800 mL | 0.9002 mL | 1.8004 mL | 4.5011 mL | |
| 25 mM | 0.1440 mL | 0.7202 mL | 1.4404 mL | 3.6009 mL | |
| 30 mM | 0.1200 mL | 0.6001 mL | 1.2003 mL | 3.0007 mL | |
| 40 mM | 0.0900 mL | 0.4501 mL | 0.9002 mL | 2.2505 mL | |
| 50 mM | 0.0720 mL | 0.3601 mL | 0.7202 mL | 1.8004 mL | |
| 60 mM | 0.0600 mL | 0.3001 mL | 0.6001 mL | 1.5004 mL | |
| 80 mM | 0.0450 mL | 0.2251 mL | 0.4501 mL | 1.1253 mL | |
| 100 mM | 0.0360 mL | 0.1800 mL | 0.3601 mL | 0.9002 mL |
Necrostatin 2 (S enantiomer) Related Classifications
HY-14622B Related Classifications
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