SPD304

Name: SPD304
Brand: MedChemExpress
SKU: HY-111255
Rating: 5.0 (7 reviews)

Cat. No.: HY-111255 Purity: 99.72%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SPD304 is a selective TNF-α inhibitor, which promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. SPD304 has an IC₅₀ of 22 μM for inhibiting in vitro TNF receptor 1 (TNFR1) binding to TNF-α.

For research use only. We do not sell to patients.

CAS No. : 869998-49-2

Size	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Oil
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of SPD304:

SPD304 dihydrochloride In-stock

7 Publications Citing Use of MCE SPD304

•Anal Bioanal Chem. 2023 Apr;415(9):1641-1655. [Abstract]

Cell Migration/Invasion Assay

Flow Cytometry

: SPD304 purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 May 9;32(1):47. [Abstract]; Representative western blot images showed the expression of KRT6A in HaCaT cells treated with high temperatures and with or without SPD304 (2μM).

: SPD304 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Aug 2:43:102187. [Abstract]; Consequently, we treated the 4NQO/EtOH group with 6 μM SPD304 for 24 h, observing significantly reduced migration and invasion capacities.

: SPD304 purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]; The selective inhibitor of TNFα, SPD304 (5, 8μM), suppressed the upregulation of MAPK pathway proteins induced by TNFα.

: SPD304 purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]; Cell proliferation, migration, and invasion experiments demonstrated that TNFα overexpression promoted proliferation, migration, and invasion of GH3 cells, while SPD304 (5, 8 μM) inhibited this effect induced by TNFα.

: SPD304 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Dec 11;11(12):1050. [Abstract]; Primary IECs were treated with 10 μg/ml mtDNA, 1 μM IFN alpha-IFNAR-IN-1 (IFNAR-IN), or 2 μM SPD304. Western blot was used to analyze necroptosis signaling.

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View All TNF Receptor Isoform Specific Products:

View All Isoforms

TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 22 μM (TNFα)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	15 μM Compound: SPD304	Inhibition of human TNFalpha induced NFkappaB activation in HEK293T cells after 6 hrs by dual-glo luciferase assay Inhibition of human TNFalpha induced NFkappaB activation in HEK293T cells after 6 hrs by dual-glo luciferase assay	10.1039/C5MD00549C
L929	IC₅₀	5 μM Compound: 1	Inhibition of human TNF assessed as reduction in TNF-induced cell death of actinomycin D activated mouse L929 cells preincubated for 30 mins followed by cell addition measured after 18 to 24 hrs by crystal violet staining based spectrophotometric analysis Inhibition of human TNF assessed as reduction in TNF-induced cell death of actinomycin D activated mouse L929 cells preincubated for 30 mins followed by cell addition measured after 18 to 24 hrs by crystal violet staining based spectrophotometric analysis	10.1039/C5MD00023H

In Vitro

SPD304 (2 μM) significantly rescues the survivability of aHSCs, reduces the production of lipid hydroxides, and increased intracellular GSH. The co-treatment of GA (75 μM) and SPD304 (2 μM), down-regulate TRADD almost 2-fold (w/o inhibitor vs. w/ inhibitor) and p RIP3 1.4 fold compared to GA alone, and promotes caspase 8 activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SPD304 cannot be used in vivo due to its high toxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

547.61

Formula

C₃₂H₃₂F₃N₃O₂

CAS No.

869998-49-2

Appearance

Oil

Color

White to off-white

SMILES

O=C1C(CN(C)CCN(C)CC2=CN(C3=CC=CC(C(F)(F)F)=C3)C4=C2C=CC=C4)=COC5=CC(C)=C(C)C=C15

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (45.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8261 mL	9.1306 mL	18.2612 mL
5 mM	0.3652 mL	1.8261 mL	3.6522 mL
10 mM	0.1826 mL	0.9131 mL	1.8261 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.28 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (2.28 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.28 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (248 KB) HNMR (145 KB) RP-HPLC (163 KB) LCMS (235 KB)

Handling Instructions (2659 KB)

References

[1]. Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005.

[2]. Alexiou P, et al. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects. Arch Pharm (Weinheim). 2014 Nov;347(11):798-805. [Content Brief]

[3]. Mouhsine H, et al. Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening. Sci Rep. 2017 Jun 13;7(1):3424. [Content Brief]

[4]. Gallic acid induces necroptosis via TNF-α signaling pathway in activated hepatic stellate cells. Chang YJ, et al. PLoS One. 2015 Mar 27;10(3):e0120713. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8261 mL	9.1306 mL	18.2612 mL	45.6529 mL
	5 mM	0.3652 mL	1.8261 mL	3.6522 mL	9.1306 mL
	10 mM	0.1826 mL	0.9131 mL	1.8261 mL	4.5653 mL
	15 mM	0.1217 mL	0.6087 mL	1.2174 mL	3.0435 mL
	20 mM	0.0913 mL	0.4565 mL	0.9131 mL	2.2826 mL
	25 mM	0.0730 mL	0.3652 mL	0.7304 mL	1.8261 mL
	30 mM	0.0609 mL	0.3044 mL	0.6087 mL	1.5218 mL
	40 mM	0.0457 mL	0.2283 mL	0.4565 mL	1.1413 mL

SPD304 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPD304869998-49-2SPD 304SPD-304TNF ReceptorTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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SPD304

Customer Review

Other Forms of SPD304:

SPD304 Related Antibodies

7 Publications Citing Use of MCE SPD304

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TNF Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

SPD304 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SPD304 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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