SPD304 dihydrochloride

Name: SPD304 dihydrochloride
Brand: MedChemExpress
SKU: HY-111255A
Rating: 5.0 (7 reviews)

Cat. No.: HY-111255A Purity: 99.82%: Data Sheet
COA

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Handling Instructions
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SPD304 dihydrochloride is a selective TNF-α inhibitor, which promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. SPD304 has an IC₅₀ of 22 μM for inhibiting in vitro TNF receptor 1 (TNFR1) binding to TNF-α.

For research use only. We do not sell to patients.

CAS No. : 1049741-03-8

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of SPD304 dihydrochloride:

SPD304 In-stock

7 Publications Citing Use of MCE SPD304 dihydrochloride

•Anal Bioanal Chem. 2023 Apr;415(9):1641-1655. [Abstract]

Cell Migration/Invasion Assay

Flow Cytometry

: SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 May 9;32(1):47. [Abstract]; Representative western blot images showed the expression of KRT6A in HaCaT cells treated with high temperatures and with or without SPD304 (2μM).

: SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Aug 2:43:102187. [Abstract]; Consequently, we treated the 4NQO/EtOH group with 6 μM SPD304 for 24 h, observing significantly reduced migration and invasion capacities.

: SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]; The selective inhibitor of TNFα, SPD304 (5, 8μM), suppressed the upregulation of MAPK pathway proteins induced by TNFα.

: SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]; Cell proliferation, migration, and invasion experiments demonstrated that TNFα overexpression promoted proliferation, migration, and invasion of GH3 cells, while SPD304 (5, 8 μM) inhibited this effect induced by TNFα.

: SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Dec 11;11(12):1050. [Abstract]; Primary IECs were treated with 10 μg/ml mtDNA, 1 μM IFN alpha-IFNAR-IN-1 (IFNAR-IN), or 2 μM SPD304. Western blot was used to analyze necroptosis signaling.

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•Related Screening Libraries:

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View All TNF Receptor Isoform Specific Products:

View All Isoforms

TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 22 μM (TNFα)^[1].

In Vitro

SPD304 (2 μM) significantly rescues the survivability of aHSCs, reduces the production of lipid hydroxides, and increased intracellular GSH. The co-treatment of GA (75 μM) and SPD304 (2 μM), down-regulate TRADD almost 2-fold (w/o inhibitor vs. w/ inhibitor) and p RIP3 1.4 fold compared to GA alone, and promotes caspase 8 activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SPD304 cannot be used in vivo due to its high toxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

620.53

Formula

C₃₂H₃₄Cl₂F₃N₃O₂

CAS No.

1049741-03-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(CN(C)CCN(C)CC2=CN(C3=CC=CC(C(F)(F)F)=C3)C4=C2C=CC=C4)=COC5=CC(C)=C(C)C=C15.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 11.36 mg/mL (18.31 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6115 mL	8.0576 mL	16.1153 mL
5 mM	0.3223 mL	1.6115 mL	3.2231 mL
10 mM	0.1612 mL	0.8058 mL	1.6115 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.82%

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Data Sheet (274 KB) SDS (393 KB)

COA (249 KB) HNMR (326 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005.

[2]. Alexiou P, et al. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects. Arch Pharm (Weinheim). 2014 Nov;347(11):798-805. [Content Brief]

[3]. Mouhsine H, et al. Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening. Sci Rep. 2017 Jun 13;7(1):3424. [Content Brief]

[4]. Gallic acid induces necroptosis via TNF-α signaling pathway in activated hepatic stellate cells. Chang YJ, et al. PLoS One. 2015 Mar 27;10(3):e0120713. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6115 mL	8.0576 mL	16.1153 mL	40.2881 mL
	5 mM	0.3223 mL	1.6115 mL	3.2231 mL	8.0576 mL
	10 mM	0.1612 mL	0.8058 mL	1.6115 mL	4.0288 mL
	15 mM	0.1074 mL	0.5372 mL	1.0744 mL	2.6859 mL