SPD304 dihydrochloride
Based on 8 publication(s) in Google Scholar
SPD304 dihydrochloride is a selective TNF-α inhibitor, which promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. SPD304 has an IC50 of 22 μM for inhibiting in vitro TNF receptor 1 (TNFR1) binding to TNF-α.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 1049741-03-8
- Formula: C32H34Cl2F3N3O2
- Molecular Weight:620.53
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Apoptosis Compound Library
HY-L003
-
Immunology/Inflammation Compound Library
HY-L007
-
Anti-Cancer Compound Library
HY-L025
-
Differentiation Inducing Compound Library
HY-L038
-
Angiogenesis-Related Compound Library
HY-L088
-
Antidepressant Compound Library
HY-L108
-
Anti-Prostate Cancer Compound Library
HY-L124
-
Anti-Pulmonary Fibrosis Compound Library
HY-L125
-
Cancer Stem Cells Compound Library
HY-L135
-
Pain-Related Compound Library
HY-L139
-
Mitochondrial Protection Compound Library
HY-L144
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Autoimmune Disease Compound Library
HY-L156
-
Highly Selective Inhibitors Library
HY-L158
-
Cytokine Inhibitors Library
HY-L161
-
Radioprotector Library
HY-L178
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Anti-Rheumatic Arthritis Compound Library
HY-L210
Publications Citing Use of MedChemExpress (MCE) SPD304 dihydrochloride
More- J Biomed Sci. 2025 May 9;32(1):47. [Abstract]
- Cell Death Dis. 2020 Dec 11;11(12):1050. [Abstract]
- Aging Cell. 2020 Oct;19(10):e13217. [Abstract]
- CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]
- J Cell Mol Med. 2026 May;30(9):e71169. [Abstract]
- FEBS J. 2022 May;289(10):2809-2827. [Abstract]
- Anal Bioanal Chem. 2023 Apr;415(9):1641-1655. [Abstract]
- Biochem Biophys Rep. 2025 Aug 2:43:102187. [Abstract]
Customer Validation & Images
-
WB
-
Cell Migration/Invasion Assay
-
WB
-
Flow Cytometry
-
WB
Biological Activity
IC50: 22 μM (TNFα)[1].
SPD304 (2 μM) significantly rescues the survivability of aHSCs, reduces the production of lipid hydroxides, and increased intracellular GSH. The co-treatment of GA (75 μM) and SPD304 (2 μM), down-regulate TRADD almost 2-fold (w/o inhibitor vs. w/ inhibitor) and p RIP3 1.4 fold compared to GA alone, and promotes caspase 8 activation[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1049741-03-8
-
Appearance Solid
-
Molecular Weight 620.53
-
Formula C32H34Cl2F3N3O2
-
Color White to off-white
-
SMILES
O=C1C(CN(C)CCN(C)CC2=CN(C3=CC=CC(C(F)(F)F)=C3)C4=C2C=CC=C4)=COC5=CC(C)=C(C)C=C15.[H]Cl.[H]Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
J Biomed Sci
2025 May 9;32(1):47. PMID: 40346694
SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 May 9;32(1):47. [Abstract]
Representative western blot images showed the expression of KRT6A in HaCaT cells treated with high temperatures and with or without SPD304 (2μM).
-
Cell Death Dis
mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusion. [Abstract]2020 Dec 11;11(12):1050. PMID: 33311495
SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Dec 11;11(12):1050. [Abstract]
Primary IECs were treated with 10 μg/ml mtDNA, 1 μM IFN alpha-IFNAR-IN-1 (IFNAR-IN), or 2 μM SPD304. Western blot was used to analyze necroptosis signaling.
-
Aging Cell
Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway. [Abstract]2020 Oct;19(10):e13217. PMID: 32840323 -
CNS Neurosci Ther
TNF-α can promote membrane invasion by activating the MAPK/MMP9 signaling pathway through autocrine in bone-invasive pituitary adenoma. [Abstract]2024 May;30(5):e14749. PMID: 38739004
SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]
The selective inhibitor of TNFα, SPD304 (5, 8μM), suppressed the upregulation of MAPK pathway proteins induced by TNFα.
SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2024 May;30(5):e14749. [Abstract]
Cell proliferation, migration, and invasion experiments demonstrated that TNFα overexpression promoted proliferation, migration, and invasion of GH3 cells, while SPD304 (5, 8 μM) inhibited this effect induced by TNFα.
-
J Cell Mol Med
Sialidase Neu3 Induced Sialic Acid Disorder and Promoted Vascular Endothelial Injury Through Transcription Factor SP3. [Abstract]2026 May;30(9):e71169. PMID: 42117319 -
FEBS J
TNF-α/ENO1 signaling facilitates testicular phagocytosis by directly activating Elmo1 gene expression in mouse Sertoli cells. [Abstract]2022 May;289(10):2809-2827. PMID: 34919331 -
Anal Bioanal Chem
Biosensor-based active ingredient recognition system for screening TNF-α inhibitors from lotus leaves. [Abstract]2023 Apr;415(9):1641-1655. PMID: 36719439 -
Biochem Biophys Rep
Network toxicology reveals collaborative mechanism of 4NQO and ethanol in esophageal squamous cell carcinogenesis. [Abstract]2025 Aug 2:43:102187. PMID: 40800605
SPD304 dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Biophys Rep. 2025 Aug 2:43:102187. [Abstract]
Consequently, we treated the 4NQO/EtOH group with 6 μM SPD304 for 24 h, observing significantly reduced migration and invasion capacities.
Solvent & Solubility
DMSO : 11.36 mg/mL (18.31 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Molly M. He, et al. Small-Molecule Inhibition of TNF-α. Science 11 Nov 2005.
[2]. Alexiou P, et al. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects. Arch Pharm (Weinheim). 2014 Nov;347(11):798-805. [Content Brief]
[3]. Mouhsine H, et al. Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening. Sci Rep. 2017 Jun 13;7(1):3424. [Content Brief]
[4]. Gallic acid induces necroptosis via TNF-α signaling pathway in activated hepatic stellate cells. Chang YJ, et al. PLoS One. 2015 Mar 27;10(3):e0120713. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6115 mL | 8.0576 mL | 16.1153 mL | 40.2881 mL |
| 5 mM | 0.3223 mL | 1.6115 mL | 3.2231 mL | 8.0576 mL | |
| 10 mM | 0.1612 mL | 0.8058 mL | 1.6115 mL | 4.0288 mL | |
| 15 mM | 0.1074 mL | 0.5372 mL | 1.0744 mL | 2.6859 mL |
SPD304 dihydrochloride Related Classifications
HY-111255A Related Classifications
Powered by Bioz