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PhIP  (Synonyms: 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine)

製品番号: HY-118716 純度: 99.93%
COA 取扱説明書 Technical Support

PhIP (2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) is the most abundant of generation of heterocyclic amines (HCA), resulted in the cooking of meat. DNA damaging and mutagenic activities. PhIP also has oestrogenic activity that could contribute to its tissue specific carcinogenicity.

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PhIP

PhIP 構造式

CAS 番号 : 105650-23-5

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 154 在庫あり
Solution
10 mM * 1 mL in DMSO USD 154 在庫あり
Solid
5 mg $140 在庫あり
10 mg $230 在庫あり
25 mg $500 在庫あり
50 mg $825 在庫あり
100 mg $1360 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Other Forms of PhIP:

PhIP 関連抗体

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製品説明

PhIP (2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) is the most abundant of generation of heterocyclic amines (HCA), resulted in the cooking of meat[1][2]. DNA damaging and mutagenic activities. PhIP also has oestrogenic activity that could contribute to its tissue specific carcinogenicity[2].

体外実験

PhIP causes widespread and largely over-lapping effects on miRNA expression. PhIP induces widespread effects via activation of oestrogen receptor alpha (ERα). Deregulation of miRNA by PhIP could potentially be an important non-DNA-damaging carcinogenic mechanism in breast cancer[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PhIP 関連抗体
体内実験

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

PhIP (400 p.p.m. in diet) induces colon carcinogenesis in male F344 rats, progressing through defined phases: PhIP-DNA adduct formation (peaking at 1-8 weeks), subsequent crypt cell hyperproliferation (4-12 weeks), and the emergence of preneoplastic aberrant crypt foci (ACF) by 14 weeks. This sequence, upon continuous exposure for 52 weeks, results in colon carcinomas at an incidence of about 50%[3][4]
PhIP (400 p.p.m. in diet), employing an intermittent high-fat regimen, efficiently induces colon carcinogenesis in male F344 rats, with an incidence of ~45% within 60 weeks[5].
PhIP (total dose 200 mg/kg, i.g.) induces the formation of small intestinal tumors at the ages of weeks 28-40 in obese hCYP1A-db/db mice[6].

Induction of colon cancer[3][4]
Background
PhIP is metabolically activated to form DNA adducts, which induce β-catenin mutations. These mutations drive β-catenin accumulation and crypt hyperproliferation, resulting in the sequential development of dysplastic ACF, adenomas, and ultimately, colon adenocarcinoma[3][4].
Specific Modeling Methods
1.Rats: F344 rats • male • 6-week-old
Administration: 400 p.p.m. in diet • p.o. • daily for 52 weeks;
2. Rats: F344 rats • male • maintained on a high-fat diet throughout • 6-week-old
Administration: 400 p.p.m. in diet • p.o. • 2 weeks, followed by 4 weeks of high-fat diet alone, the cycle repeated 3 times, followed by the high-fat diet for 42 weeks
Note
In Modeling Method 2: After completing the 3 cycles, animals continued on the high-fat diet alone for an additional 42 weeks.
Modeling Indicators
Molecular changes: PhIP-DNA adduct formation, β-Catenin mutation (e.g., at codon 34), cytoplasmic β-catenin accumulation, increased Ki-67 level
Cellular changes: Hyperproliferation of crypt epithelial cells
Histology analysis: Dysplastic ACF, Crypt distortion


Induction of small intestinal carcinogenesis[6]
Background
The combination of PhIP-induced Apc mutation and obesity-associated DNA hypermethylation silences Apc function, driving nuclear β-catenin accumulation, crypt hyperproliferation, and ultimately the formation of dysplastic tubular adenomas in the small intestine[6].
Specific Modeling Methods
Mice: hCYP1A-db/db mice • fed with AIN93M diet • 6 week-old
Administration: 100 mg/kg • i.g. • total 2 doses, 4 days apart, starting at 6 weeks of age.
Note
Mice were continuously maintained on the AIN93M diet until the termination of the experiment (28-40 weeks after the PhIP treatment).
Modeling Indicators
Molecular changes: Upregulation of NF-κB (p65), pSTAT3, and COX2 in small intestinal tumors; nuclear β-catenin accumulation
Cellular changes: Hyperproliferation of crypt epithelial cells
Histology analysis: tubular adenomas (with dysplasia), Crypt distortion


In hCYP1A-mice, PhIP induces inflammation, epithelial cell damage, and prostatic intraepithelial neoplasia in the dorsolateral prostate lobe compared to the ventral lobe. PhIP forms DNA adducts in the prostate, PhIP also induces oxidative stress, atrophy of the acini, and inflammation of the prostate of rodents[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

224.26

分子式

C13H12N4
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

NC1=NC2=NC=C(C=C2N1C)C3=CC=CC=C3
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (111.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4591 mL 22.2956 mL 44.5911 mL
5 mM 0.8918 mL 4.4591 mL 8.9182 mL
10 mM 0.4459 mL 2.2296 mL 4.4591 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.56 mg/mL (2.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 0.56 mg/mL (2.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.93%

COA (246 KB) LCMS (89 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4591 mL 22.2956 mL 44.5911 mL 111.4778 mL
5 mM 0.8918 mL 4.4591 mL 8.9182 mL 22.2956 mL
10 mM 0.4459 mL 2.2296 mL 4.4591 mL 11.1478 mL
15 mM 0.2973 mL 1.4864 mL 2.9727 mL 7.4319 mL
20 mM 0.2230 mL 1.1148 mL 2.2296 mL 5.5739 mL
25 mM 0.1784 mL 0.8918 mL 1.7836 mL 4.4591 mL
30 mM 0.1486 mL 0.7432 mL 1.4864 mL 3.7159 mL
40 mM 0.1115 mL 0.5574 mL 1.1148 mL 2.7869 mL
50 mM 0.0892 mL 0.4459 mL 0.8918 mL 2.2296 mL
60 mM 0.0743 mL 0.3716 mL 0.7432 mL 1.8580 mL
80 mM 0.0557 mL 0.2787 mL 0.5574 mL 1.3935 mL
100 mM 0.0446 mL 0.2230 mL 0.4459 mL 1.1148 mL
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PhIP Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PhIP105650-23-52-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridineOthersInhibitorinhibitorinhibit

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