2. Others Anti-infection
  3. Environmental Pollutants SARS-CoV
  4. Propiolactone

Propiolactone  (Synonyms: β-propiolactone; 2-Oxetanone; Betaprone)

Art. -Nr.: HY-107931 Reinheit: 99.36%
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Handling Instructions Technical Support

Propiolactone (β-propiolactone; 2-Oxetanone) is a viral chemical inactivator that causes the infectious inactivation of viruses. Propiolactone was co-incubated with SARS-CoV at a ratio of 1:1000 (v:v) and used as a bacteriostatic agent to formulate the BPL-inactivated influenza virus vaccine (Flu-BPL).

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Propiolactone

Propiolactone Chemische Struktur

CAS. Nr. : 57-57-8

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Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Other Forms of Propiolactone:

Propiolactone Related Antibodies

Top Publications Citing Use of Products

  • Biologische Aktivität

  • Reinheit & Dokumentation

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Beschreibung

Propiolactone (β-propiolactone; 2-Oxetanone) is a viral chemical inactivator that causes the infectious inactivation of viruses. Propiolactone was co-incubated with SARS-CoV at a ratio of 1:1000 (v:v) and used as a bacteriostatic agent to formulate the BPL-inactivated influenza virus vaccine (Flu-BPL)[1][2].

IC50 & Target

SARS-CoV-2[1]
In Vitro

Propiolactone (β-propiolactone) can be used for vaccine purification. After cells were harvested by low-speed centrifugation, SARS-CoV was chemically inactivated with Propiolactone (1:1000 v:v). Propiolactone was incubated with SARS-CoV for 24 h at 4°C. A second incubation at room temperature was performed to hydrolyze residual propiolactone. and concentration of the vaccine. Following BPL inactivation. a polvethylene glycol-sodium chloride (PEG-NaCl) mixture was added to precipitate the inactivated virus. After cen and concentration of the vaccine. After propiolactone inactivation, a polyethylene glycol-sodium chloride (PEG-NaCl) mixture is added to precipitate the inactivated virus. Finally, Propiolactone (1:10000 v:v) was added as a bacteriostatic agent.
Propiolactone-inactivated virus loses infectivity in Vero cells[1].
Note: Propiolactone is unstable in water and prone to hydrolysis. It should be used immediately after being dissolved in water.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Propiolactone Related Antibodies
In Vivo

Mice were immunized with propiolactone (β-propiolactone)-inactivated influenza A virus (~25 mg total protein per dose; intramuscular injection). SARS is non-lethal in young BALB/c mice after propiolactone inactivation treatment. Although the virus replicated in the respiratory tract of the mice, it was cleared by day 5. Propiolactone treatment yielded 1.5 μg of total hemagglutinin protein, which was negative after infection of mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molekulargewicht

72.06

Formel

C3H4O2
CAS. Nr.
Appearance

Liquid (Density: 1.146 g/cm3)

Color

Colorless to light yellow

SMILES

O=C1OCC1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 100 mg/mL (1387.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 13.8773 mL 69.3866 mL 138.7733 mL
5 mM 2.7755 mL 13.8773 mL 27.7547 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (34.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (34.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation

Purity: 99.36%

SDS (647 KB)

COA (249 KB) HNMR (222 KB) GC (180 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 13.8773 mL 69.3866 mL 138.7732 mL 346.9331 mL
5 mM 2.7755 mL 13.8773 mL 27.7546 mL 69.3866 mL
10 mM 1.3877 mL 6.9387 mL 13.8773 mL 34.6933 mL
15 mM 0.9252 mL 4.6258 mL 9.2515 mL 23.1289 mL
20 mM 0.6939 mL 3.4693 mL 6.9387 mL 17.3467 mL
25 mM 0.5551 mL 2.7755 mL 5.5509 mL 13.8773 mL
30 mM 0.4626 mL 2.3129 mL 4.6258 mL 11.5644 mL
40 mM 0.3469 mL 1.7347 mL 3.4693 mL 8.6733 mL
50 mM 0.2775 mL 1.3877 mL 2.7755 mL 6.9387 mL
60 mM 0.2313 mL 1.1564 mL 2.3129 mL 5.7822 mL
80 mM 0.1735 mL 0.8673 mL 1.7347 mL 4.3367 mL
100 mM 0.1388 mL 0.6939 mL 1.3877 mL 3.4693 mL
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Propiolactone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Propiolactone57-57-8β-propiolactone 2-Oxetanone BetaproneEnvironmental PollutantsSARS-CoVEnvironmental ContaminantsSARS coronavirusAntibodyCOVID-19ELISAIgGInactivated virusSARS-CoV-2Inhibitorinhibitorinhibit

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