Succinyl-CoA synthetase 

Succinyl-CoA synthetase is a mitochondrial matrix enzyme and catalyst. Succinyl-CoA synthetase supports TCA, ketone and heme metabolism and is activated by mitochondrial phosphate. Succinyl-CoA synthetase distributes broadly across mammalian tissues with distinct substrate-related biochemical features. Succinyl-CoA synthetase gains structural stability after phosphorylation and relies on SUCLG1-encoded subunit for activity. Succinyl-CoA synthetase malfunction links to metabolic and neurological disorders. Succinyl-CoA synthetase serves as a research tool for mitochondrial hepatoencephalomyopathy^[1][2][3].

Succinyl-CoA synthetase exists as a tetramer, composed of two distinct subunits with molecular weights of 42500 and 34500 respectively; the smaller α subunit contains a histidine phosphorylation active site^[1][2].
Succinyl-CoA synthetase gains improved structural stability and resistance to storage-related deactivation and trypsin-dependent proteolysis after phosphorylation-triggered conformational alterations^[2].
Succinyl-CoA synthetase exhibits specificity for succinate at standard concentrations (malate, itaconate, and α-methylsuccinate can serve as weak or equivalently active alternative substrates), accepts dephospho-coenzyme A as a weak substitute for coenzyme A, and shows relaxed specificity for purine nucleotides. Its intrinsic purine nucleoside diphosphate kinase activity is regulated by succinyl-CoA and ATP^[2].
Succinyl-CoA synthetase (1 mg protein/mL; 10 min) shows severely deficient activityin cultured human dermal fibroblasts with homozygous SUCLG c.40A > T mutation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9080-33-5

Liquid

Colorless to light yellow

[Succinyl-CoA synthetase]

6.2.1.4

8 U/mg protein

One Unit is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in glycylglycine buffer (34 mM), pH 8.4 at 25°C).

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Li X, et al. Identification of the kinetic mechanism of succinyl-CoA synthetase. Biosci Rep. 2013;33(1):145-163. Published 2013 Jan 18.

  [2]. Bridger WA. Succinyl-Coa Synthetase. In: Boyer PD, editor. The Enzymes. Vol 10; 1974. p.581-606.

  [3]. Van Hove JL, et al. Succinyl-CoA ligase deficiency: a mitochondrial hepatoencephalomyopathy. Pediatr Res. 2010;68(2):159-164.