2. Metabolic Enzyme/Protease
  3. E1/E2/E3 Enzyme
  4. UBA5-IN-1

UBA5-IN-1 (compound 8.5) is a selective UBA5 inhibitor with an IC50 value of 4.0 μM. UBA5-IN-1 inhibits cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5.

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UBA5-IN-1

UBA5-IN-1 構造式

CAS 番号 : 1831169-11-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1405 在庫あり
Solution
10 mM * 1 mL in DMSO USD 1405 在庫あり
Solid
5 mg $700 在庫あり
10 mg $1120 在庫あり
25 mg $2250 在庫あり
50 mg $3650 在庫あり
100 mg $5900 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

UBA5-IN-1 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

UBA5-IN-1 (compound 8.5) is a selective UBA5 inhibitor with an IC50 value of 4.0 μM. UBA5-IN-1 inhibits cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5[1].

IC50 & Target

IC50: 4.0 μM (UBA5), 78.5 μM (UAE), 66.8 μM (NAE)[1]
体外実験

UBA5-IN-1 (0.1-1000 μM) shows dose-dependent inhibition effect to UBA5 with an IC50 value of 4.0 μM, and inhibits UAE and NAE with IC50 values of 78.5 and 66.8 μM, respectively[1].
UBA5-IN-1 (0-5 μM) inhibits UBA5 non-competitively with respect to ATP[1].
UBA5-IN-1 (10 μM) shows no effect on ATP binding to human kinase[1].
UBA5-IN-1 (0-50 μM; 72 h) effects cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

UBA5-IN-1 関連抗体

Cell Proliferation Assay[1]

Cell Line: Sk-Luci6 cancer cells with high expression levels of UBA5
Concentration: 0-50 μM
Incubation Time: 0-72 h
Result: Showed selective anti-proliferative activity for Sk-Luci6 cancer cells which expressed higher UBA5 protein levels, but showed no anti-proliferative activity to MRC9 lung fibroblasts and A549 carcinoma cells.
分子量

912.16

分子式

C26H40F6N10O11S2Zn
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=S(C(F)(F)F)([O-])=O.O=S(C(F)(F)F)([O-])=O.NC1=C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](C(NCCCCCC([N]45[Zn+2]67[N@@](CC[N]6([H])CC[N@]7([H])CC5)([H])CC4)=O)=O)O3)C2=NC=N1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMF : 100 mg/mL (109.63 mM; Need ultrasonic)

H2O : 50 mg/mL (54.81 mM; Need ultrasonic)

DMSO : 50 mg/mL (54.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0963 mL 5.4815 mL 10.9630 mL
5 mM 0.2193 mL 1.0963 mL 2.1926 mL
10 mM 0.1096 mL 0.5481 mL 1.0963 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (1.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (1.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO / DMF 1 mM 1.0963 mL 5.4815 mL 10.9630 mL 27.4075 mL
5 mM 0.2193 mL 1.0963 mL 2.1926 mL 5.4815 mL
10 mM 0.1096 mL 0.5481 mL 1.0963 mL 2.7407 mL
15 mM 0.0731 mL 0.3654 mL 0.7309 mL 1.8272 mL
20 mM 0.0548 mL 0.2741 mL 0.5481 mL 1.3704 mL
25 mM 0.0439 mL 0.2193 mL 0.4385 mL 1.0963 mL
30 mM 0.0365 mL 0.1827 mL 0.3654 mL 0.9136 mL
40 mM 0.0274 mL 0.1370 mL 0.2741 mL 0.6852 mL
50 mM 0.0219 mL 0.1096 mL 0.2193 mL 0.5481 mL
DMF 60 mM 0.0183 mL 0.0914 mL 0.1827 mL 0.4568 mL
80 mM 0.0137 mL 0.0685 mL 0.1370 mL 0.3426 mL
100 mM 0.0110 mL 0.0548 mL 0.1096 mL 0.2741 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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UBA5-IN-1 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

UBA5-IN-11831169-11-9E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseSk-Luci6UAENAEATPhuman kinaseMRC9A549Inhibitorinhibitorinhibit

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製品名:
UBA5-IN-1
製品番号:
HY-148266
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