3-Deazaadenosine (Synonyms: 3-デアザアデノシン)

Name: 3-Deazaadenosine
Brand: MedChemExpress
SKU: HY-W013332
Rating: 5.0 (27 reviews)

製品番号: HY-W013332 純度: 98.53%: Data Sheet SDS COA 取扱説明書
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3-Deazaadenosine is an inhibitor of S-adenosylhomocysteine hydrolase, with a K_i of 3.9 μM; 3-Deazaadenosine has anti-inflammatory, anti-proliferative and anti-HIV activity.

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CAS 番号 : 6736-58-9

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 308	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 308	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$160	在庫あり	Estimated Time of Arrival: December 31
5 mg	$280	在庫あり	Estimated Time of Arrival: December 31
10 mg	$480	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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カスタマーレビュー

Based on 27 publication(s) in Google Scholar

Other Forms of 3-Deazaadenosine:

3-Deazaadenosine hydrochloride 在庫あり

顧客検証

RT-PCR

: 3-Deazaadenosine purchased from MedChemExpress. Usage Cited in: Arch Toxicol. 2021 Jan;95(1):299-309. [Abstract]; After treating with 3-Deazaadenosine (DAA, 50 mM), the global methylation inhibitor, EJ and J82 cell lines show decreased m6A modifcation level and reduced SOD2 expression level.

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HIV HIV-1 HIV-2

生物活性
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製品説明

3-Deazaadenosine is an inhibitor of S-adenosylhomocysteine hydrolase, with a K_i of 3.9 μM; 3-Deazaadenosine has anti-inflammatory, anti-proliferative and anti-HIV activity.

IC₅₀ & Target

HIV-1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	57.37 3 Compound: 3-DZA	Cytotoxicity against human A549 cells assessed as cell growth inhibition Cytotoxicity against human A549 cells assessed as cell growth inhibition	[PMID: 35809817]
A549	IC₅₀	57.37 3 Compound: 3-DZA	Cytotoxicity against human A549 cells assessed as cell growth inhibition Cytotoxicity against human A549 cells assessed as cell growth inhibition	[PMID: 35809817]
HEp-2	IC₅₀	2 3 Compound: 2	Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control. Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control.	[PMID: 6708054]
L02	IC₅₀	94.43 3 Compound: 3-DZA	Cytotoxicity against human L02 cells assessed as cell growth inhibition Cytotoxicity against human L02 cells assessed as cell growth inhibition	[PMID: 35809817]
MCF7	IC₅₀	40.32 3 Compound: 3-DZA	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition Cytotoxicity against human MCF7 cells assessed as cell growth inhibition	[PMID: 35809817]
MGC-803	IC₅₀	41.06 3 Compound: 3-DZA	Cytotoxicity against human MGC-803 cells assessed as cell growth inhibition Cytotoxicity against human MGC-803 cells assessed as cell growth inhibition	[PMID: 35809817]
MCF7	IC₅₀	<100 1 Compound: 6, DZA	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 26314922]
MDA-MB-231	IC₅₀	<100 1 Compound: 6, DZA	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 26314922]
SK-BR-3	IC₅₀	<100 1 Compound: 6, DZA	Cytotoxicity against human SKBR3 cells Cytotoxicity against human SKBR3 cells	[PMID: 26314922]
HEp-2	IC₅₀	2 3 Compound: 2	Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control. Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control.	[PMID: 6708054]
L02	IC₅₀	94.43 3 Compound: 3-DZA	Cytotoxicity against human L02 cells assessed as cell growth inhibition Cytotoxicity against human L02 cells assessed as cell growth inhibition	[PMID: 35809817]
MCF7	IC₅₀	40.32 3 Compound: 3-DZA	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition Cytotoxicity against human MCF7 cells assessed as cell growth inhibition	[PMID: 35809817]
MCF7	IC₅₀	< 100 1 Compound: 6, DZA	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 26314922]
MDA-MB-231	IC₅₀	< 100 1 Compound: 6, DZA	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 26314922]
MGC-803	IC₅₀	41.06 3 Compound: 3-DZA	Cytotoxicity against human MGC-803 cells assessed as cell growth inhibition Cytotoxicity against human MGC-803 cells assessed as cell growth inhibition	[PMID: 35809817]
SK-BR-3	IC₅₀	< 100 1 Compound: 6, DZA	Cytotoxicity against human SKBR3 cells Cytotoxicity against human SKBR3 cells	[PMID: 26314922]

体外実験

3-Deazaadenosine is an inhibitor of S-adenosylhomocysteine hydrolase, with a K_i of 3.9 μM. 3-Deazaadenosine shows anti-HIV effect, and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018) with IC₅₀s of 0.15 and 0.20 μM, respectively^[1]. 3-Deazaadenosine (1-100 μM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine^[2]. 3-Deazaadenosine (50, 100 μM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 μM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

266.26

分子式

C₁₁H₁₄N₄O₄

CAS 番号

6736-58-9

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C3=C(C(N)=NC=C3)N=C2

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 130 mg/mL (488.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (375.57 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7557 mL	18.7786 mL	37.5573 mL
5 mM	0.7511 mL	3.7557 mL	7.5115 mL
10 mM	0.3756 mL	1.8779 mL	3.7557 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (8.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (8.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (8.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: ≥99.0%

Select Batch:

データシート (278 KB) SDS (420 KB)

COA (249 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Gordon RK, et al. Anti-HIV-1 activity of 3-deaza-adenosine analogs. Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners. Eur J Biochem. 2003 Sep;270(17):3507-17. [Content Brief]

[2]. Jeong SY, et al. 3-deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-kappaB regulation. Inhibition of NF-kappaB transcriptional activity and promotion of IkappaBalpha degradation. J Biol Chem. 1999 Jul 2;274(27):18981-8. [Content Brief]

[3]. Sedding DG, et al. 3-Deazaadenosine prevents smooth muscle cell proliferation and neointima formation by interfering with Ras signaling. Circ Res. 2009 May 22;104(10):1192-200. [Content Brief]

細胞実験
^[1]

The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID₅₀) of the virus stock per 2 × 10⁵ PBMC cells. The TCID₅₀ is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 10⁵ cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA^[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Gordon RK, et al. Anti-HIV-1 activity of 3-deaza-adenosine analogs. Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners. Eur J Biochem. 2003 Sep;270(17):3507-17. [Content Brief]

[2]. Jeong SY, et al. 3-deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-kappaB regulation. Inhibition of NF-kappaB transcriptional activity and promotion of IkappaBalpha degradation. J Biol Chem. 1999 Jul 2;274(27):18981-8. [Content Brief]

[3]. Sedding DG, et al. 3-Deazaadenosine prevents smooth muscle cell proliferation and neointima formation by interfering with Ras signaling. Circ Res. 2009 May 22;104(10):1192-200. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.7557 mL	18.7786 mL	37.5573 mL	93.8932 mL
	5 mM	0.7511 mL	3.7557 mL	7.5115 mL	18.7786 mL
	10 mM	0.3756 mL	1.8779 mL	3.7557 mL	9.3893 mL
	15 mM	0.2504 mL	1.2519 mL	2.5038 mL	6.2595 mL
	20 mM	0.1878 mL	0.9389 mL	1.8779 mL	4.6947 mL
	25 mM	0.1502 mL	0.7511 mL	1.5023 mL	3.7557 mL
	30 mM	0.1252 mL	0.6260 mL	1.2519 mL	3.1298 mL
	40 mM	0.0939 mL	0.4695 mL	0.9389 mL	2.3473 mL
	50 mM	0.0751 mL	0.3756 mL	0.7511 mL	1.8779 mL
	60 mM	0.0626 mL	0.3130 mL	0.6260 mL	1.5649 mL
	80 mM	0.0469 mL	0.2347 mL	0.4695 mL	1.1737 mL
	100 mM	0.0376 mL	0.1878 mL	0.3756 mL	0.9389 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

3-Deazaadenosine Related Classifications

Inflammation/Immunology Infection

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Deazaadenosine6736-58-9S-adenosylhomocysteine hydrolase (SAHH)HIVSAHHHuman immunodeficiency virusInhibitorinhibitorinhibit

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3-Deazaadenosine (Synonyms: 3-デアザアデノシン)

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Other Forms of 3-Deazaadenosine:

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参考文献

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

3-Deazaadenosine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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3-Deazaadenosine (Synonyms: 3-デアザアデノシン)

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Other Forms of 3-Deazaadenosine:

3-Deazaadenosine 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

HIV アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

3-Deazaadenosine 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

3-Deazaadenosine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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