3-Deazaadenosine hydrochloride
Based on 32 publication(s) in Google Scholar
3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 μM; 3-Deazaadenosine has anti-inflammatory, anti-proliferative and anti-HIV activity.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 86583-19-9
- Formula: C11H15ClN4O4
- Molecular Weight:302.72
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Publications Citing Use of MedChemExpress (MCE) 3-Deazaadenosine hydrochloride
More- Cancer Res. 2021 Sep 15;81(18):4778-4793. [Abstract]
- J Clin Invest. 2025 Jul 22;135(19):e190215. [Abstract]
- Adv Sci (Weinh). 2026 Jun 18:e76122. [Abstract]
- Mol Biomed. 2026 Apr 8;7(1):47. [Abstract]
- Cell Death Dis. 2025 Oct 27;16(1):767. [Abstract]
- Cell Mol Biol Lett. 2024 Jul 3;29(1):97. [Abstract]
- Int J Biol Sci. 2023 Jan 1;19(2):593-609. [Abstract]
- Arch Toxicol. 2021 Jan;95(1):299-309. [Abstract]
- Oncogene. 2024 Apr;43(17):1288-1302. [Abstract]
- Cell Rep. 2021 Jan 5;34(1):108580. [Abstract]
- Environ Pollut. 2023 Aug 15;331(Pt 2):121798. [Abstract]
- Atherosclerosis. 2026 May 14:417:120788. [Abstract]
- Cancer Cell Int. 2023 Dec 18;23(1):330. [Abstract]
- Virulence. 2026 Dec;17(1):2645908. [Abstract]
- Cancer Gene Ther. 2026 Mar;33(3):277-288. [Abstract]
- Eur J Pharmacol. 2025 Feb 15:989:177247. [Abstract]
- Biomolecules. 2025 Aug 18;15(8):1188. [Abstract]
- Inflammation. 2025 Nov 7. [Abstract]
- BMC Biol. 2024 May 3;22(1):104. [Abstract]
- Am J Physiol Cell Physiol. 2024 Mar 1;326(3):C684-C697. [Abstract]
- Cell Signal. 2025 Mar:127:111623. [Abstract]
- Water Biol Secur. 2025 Apr 18.
- J Neurosci. 2023 Mar 1;43(9):1456-1474. [Abstract]
- Genomics. 2022 Jul;114(4):110424. [Abstract]
- Cell Biol Int. 2025 Jun;49(6):674-691. [Abstract]
- Exp Cell Res. 2025 May 1;448(1):114538. [Abstract]
- Exp Cell Res. 2024 Oct 1;442(2):114226. [Abstract]
- J Cancer. 2023 Apr 17;14(7):1117-1131. [Abstract]
- Oncol Lett. 2026 Apr 20;31(6):254. [Abstract]
- Transpl Immunol. 2024 Oct:86:102083. [Abstract]
- bioRxiv. 2024 May 18.
- Research Square Print. 2022 May.
Customer Validation & Images
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RT-PCR
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RT-PCR
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WB
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ELISA
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WB
Biological Activity
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HIV-1 |
3-Deazaadenosine is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 μM. 3-Deazaadenosine shows anti-HIV effect, and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018) with IC50s of 0.15 and 0.20 μM, respectively[1].3-Deazaadenosine (1-100 μM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine[2]. 3-Deazaadenosine (50, 100 μM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 μM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 86583-19-9
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Appearance Solid
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Molecular Weight 302.72
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Formula C11H15ClN4O4
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C3=C(C(N)=NC=C3)N=C2.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (32)
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Journal Impact Factor
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Most Recent
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Cancer Res
M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma. [Abstract]2021 Sep 15;81(18):4778-4793. PMID: 34301762 -
J Clin Invest
Coenzyme A protects against ferroptosis via CoAlation of mitochondrial thioredoxin reductase. [Abstract]2025 Jul 22;135(19):e190215. PMID: 40694424 -
Adv Sci (Weinh)
ZNF33B Promotes Japanese Encephalitis Virus Infection by Regulating the Stability of M6A-Modified Trim25 to Control the Autophagy Process. [Abstract]2026 Jun 18:e76122. PMID: 42314059 -
Mol Biomed
Targeting the RNA-binding motif protein 15 suppresses prostate cancer progression and hormone therapy resistance by promoting androgen receptor degradation. [Abstract]2026 Apr 8;7(1):47. PMID: 41949706 -
Cell Death Dis
TRIM17 promotes the progression of osteosarcoma by regulating PDK1 m6A modification-mediated AKT/mTOR pathway activation through ubiquitination of FTO. [Abstract]2025 Oct 27;16(1):767. PMID: 41145484
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 27;16(1):767. [Abstract]
3-Deazaadenosine (DAA: 1 μM 48 h). PDK1 mRNA levels were detected by qRT-PCR.
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Cell Mol Biol Lett
WTAP/IGF2BP3-mediated GBE1 expression accelerates the proliferation and enhances stemness in pancreatic cancer cells via upregulating c-Myc. [Abstract]2024 Jul 3;29(1):97. PMID: 38961325
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2024 Jul 3;29(1):97. [Abstract]
The mRNA expression level of GBE1 in BXPC3 cells after 3-Deazaadenosine (DAA) treatment.
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Biol Lett. 2024 Jul 3;29(1):97. [Abstract]
Protein expression level of GBE1 in BXPC3 cells after 3-Deazaadenosine (DAA) treatment.
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Int J Biol Sci
Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway. [Abstract]2023 Jan 1;19(2):593-609. PMID: 36632449
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Jan 1;19(2):593-609. [Abstract]
The protein levels of E2F1 and MIF were measured using WB in HK2 cells with IGF2BP1 knocked down, treated with or without 3-Deazaadenosine (DAA) for 12 h.
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Arch Toxicol
Genetic variants in N6-methyladenosine are associated with bladder cancer risk in the Chinese population. [Abstract]2021 Jan;95(1):299-309. PMID: 32964246
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Arch Toxicol. 2021 Jan;95(1):299-309. [Abstract]
After treating with 3-Deazaadenosine (DAA, 50 mM), the global methylation inhibitor, EJ and J82 cell lines show decreased m6A modifcation level and reduced SOD2 expression level.
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Oncogene
N6-methyladenosine demethyltransferase FTO mediated m6A modification of estrogen receptor alpha in non-small cell lung cancer tumorigenesis. [Abstract]2024 Apr;43(17):1288-1302. PMID: 38443681
3-Deazaadenosine hydrochloride purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Apr;43(17):1288-1302. [Abstract]
The expression level of ESR1 protein in xenograft tumor tissues of the DMSO, 3-Deazaadenosine (DAA: 50 mg/kg) or AZD9496 treatment groups was detected by ELISA.
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Cell Rep
RNA m6A Modification Functions in Larval Development and Caste Differentiation in Honeybee (Apis mellifera). [Abstract]2021 Jan 5;34(1):108580. PMID: 33406439 -
Environ Pollut
linc01515 regulates PM2.5-induced oxidative stress via targeting NRF2 in airway epithelial cells. [Abstract]2023 Aug 15;331(Pt 2):121798. PMID: 37169236 -
Atherosclerosis
The m6A demethylase FTO suppresses perivascular adipose tissue browning and exacerbates vascular injury via m6A-mediated destabilization of Irx3 mRNA and the IRX3/UCP1 axis. [Abstract]2026 May 14:417:120788. PMID: 42155396 -
Cancer Cell Int
LncRNA AGAP2 antisense RNA 1 stabilized by insulin-like growth factor 2 mRNA binding protein 3 promotes macrophage M2 polarization in clear cell renal cell carcinoma through regulation of the microRNA-9-5p/THBS2/PI3K-Akt pathway. [Abstract]2023 Dec 18;23(1):330. PMID: 38110984 -
Virulence
HIV-1 Vpr activates microglia by upregulating m6A modification through ubiquitin-proteasome pathway-mediated degradation of the demethylase ALKBH5. [Abstract]2026 Dec;17(1):2645908. PMID: 41824629 -
Cancer Gene Ther
WTAP stabilized by USP7 contributes to enzalutamide resistance in prostate cancer via mediating AKT m6A-modification. [Abstract]2026 Mar;33(3):277-288. PMID: 41981278 -
Eur J Pharmacol
Targeting the ALKBH5-NLRP3 positive feedback loop alleviates cardiomyocyte pyroptosis after myocardial infarction. [Abstract]2025 Feb 15:989:177247. PMID: 39746531 -
Biomolecules
Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS. [Abstract]2025 Aug 18;15(8):1188. PMID: 40867632 -
Inflammation
HNRNPC-Mediated m6A Epitranscriptomics Drives CD80-Dependent Tubular Dysfunction in Sepsis-Induced AKI. [Abstract]2025 Nov 7. PMID: 41199093 -
BMC Biol
GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. [Abstract]2024 May 3;22(1):104. PMID: 38702712 -
Am J Physiol Cell Physiol
ALKHB5-demethylated lncRNA SNHG15 promotes myeloma tumorigenicity by increasing chromatin accessibility and recruiting H3K36me3 modifier SETD2. [Abstract]2024 Mar 1;326(3):C684-C697. PMID: 38145297 -
Cell Signal
METTL14-mediated depression of NEIL1 aggravates oxidative damage and mitochondrial dysfunction of lens epithelial cells through regulating KEAP1/NRF2 pathways. [Abstract]2025 Mar:127:111623. PMID: 39855533 -
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J Neurosci
USP1/UAF1-stabilized METTL3 promotes reactive astrogliosis and improves functional recovery after spinal cord injury through m6A modification of YAP1 mRNA. [Abstract]2023 Mar 1;43(9):1456-1474. PMID: 36653190 -
Genomics
SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT. [Abstract]2022 Jul;114(4):110424. PMID: 35798250 -
Cell Biol Int
FLOT1 Is a Novel Serum Biomarker of Ovarian Cancer Targeted by N6-methyladenosine Modification Inhibition. [Abstract]2025 Jun;49(6):674-691. PMID: 40066501 -
Exp Cell Res
Irradiation of 125I seeds blocks glycolysis in pancreatic cancer by inhibiting KLF5 m6A methylation through the suppression of RBM15. [Abstract]2025 May 1;448(1):114538. PMID: 40147709 -
Exp Cell Res
N6-methyladenosine modification of linc-OIP5 confers paclitaxel resistance in breast cancer through a DDX5-dependent mechanism. [Abstract]2024 Oct 1;442(2):114226. PMID: 39209141 -
J Cancer
METTL14 Facilitates the Metastasis of Pancreatic Carcinoma by Stabilizing LINC00941 in an m6A-IGF2BP2-Dependent Manner. [Abstract]2023 Apr 17;14(7):1117-1131. PMID: 37215454 -
Oncol Lett
METTL5 reprograms glycolytic metabolism and promotes non-small cell lung cancer progression by modifying PGAM1. [Abstract]2026 Apr 20;31(6):254. PMID: 42057886 -
Transpl Immunol
Bushen Huoxue decotion-containing serum prevents chondrocyte pyroptosis in a m6A-dependent manner in facet joint osteoarthritis. [Abstract]2024 Oct:86:102083. PMID: 38996984 -
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Solvent & Solubility
H2O : ≥ 100 mg/mL (330.34 mM)
DMSO : 41.67 mg/mL (137.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 105 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 105 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Gordon RK, et al. Anti-HIV-1 activity of 3-deaza-adenosine analogs. Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners. Eur J Biochem. 2003 Sep;270(17):3507-17. [Content Brief]
[2]. Jeong SY, et al. 3-deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-kappaB regulation. Inhibition of NF-kappaB transcriptional activity and promotion of IkappaBalpha degradation. J Biol Chem. 1999 Jul 2;274(27):18981-8. [Content Brief]
[3]. Sedding DG, et al. 3-Deazaadenosine prevents smooth muscle cell proliferation and neointima formation by interfering with Ras signaling. Circ Res. 2009 May 22;104(10):1192-200. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.3034 mL | 16.5169 mL | 33.0338 mL | 82.5846 mL |
| 5 mM | 0.6607 mL | 3.3034 mL | 6.6068 mL | 16.5169 mL | |
| 10 mM | 0.3303 mL | 1.6517 mL | 3.3034 mL | 8.2585 mL | |
| 15 mM | 0.2202 mL | 1.1011 mL | 2.2023 mL | 5.5056 mL | |
| 20 mM | 0.1652 mL | 0.8258 mL | 1.6517 mL | 4.1292 mL | |
| 25 mM | 0.1321 mL | 0.6607 mL | 1.3214 mL | 3.3034 mL | |
| 30 mM | 0.1101 mL | 0.5506 mL | 1.1011 mL | 2.7528 mL | |
| 40 mM | 0.0826 mL | 0.4129 mL | 0.8258 mL | 2.0646 mL | |
| 50 mM | 0.0661 mL | 0.3303 mL | 0.6607 mL | 1.6517 mL | |
| 60 mM | 0.0551 mL | 0.2753 mL | 0.5506 mL | 1.3764 mL | |
| 80 mM | 0.0413 mL | 0.2065 mL | 0.4129 mL | 1.0323 mL | |
| 100 mM | 0.0330 mL | 0.1652 mL | 0.3303 mL | 0.8258 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
3-Deazaadenosine hydrochloride Related Classifications
HY-W013332A Related Classifications
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