1. Anti-infection
  2. HIV

3-Deazaadenosine hydrochloride 

Cat. No.: HY-W013332A Purity: 98.06%
Handling Instructions

3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 µM; 3-Deazaadenosine has anti-inflammatory, anti-proliferative and anti-HIV activity.

For research use only. We do not sell to patients.

3-Deazaadenosine hydrochloride Chemical Structure

3-Deazaadenosine hydrochloride Chemical Structure

CAS No. : 86583-19-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 186 In-stock
Estimated Time of Arrival: December 31
1 mg USD 160 In-stock
Estimated Time of Arrival: December 31
5 mg USD 280 In-stock
Estimated Time of Arrival: December 31
10 mg USD 480 Get quote
50 mg   Get quote  
100 mg   Get quote  

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Other Forms of 3-Deazaadenosine hydrochloride:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 µM; 3-Deazaadenosine has anti-inflammatory, anti-proliferative and anti-HIV activity.

IC50 & Target

IC50: 0.15 (HIV-1, A012 isolate), 0.20 µM (HIV-1, A018 isolate)[1]
Ki: 3.9 µM (S-adenosylhomocysteine hydrolase)[1]

In Vitro

3-Deazaadenosine is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 µM. 3-Deazaadenosine shows anti-HIV effect, and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018) with IC50s of 0.15 and 0.20 µM, respectively[1]. 3-Deazaadenosine (1-100 µM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 µM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine[2]. 3-Deazaadenosine (50, 100 µM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 µM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling[3].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3035 mL 16.5175 mL 33.0349 mL
5 mM 0.6607 mL 3.3035 mL 6.6070 mL
10 mM 0.3303 mL 1.6517 mL 3.3035 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 105 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 105 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

302.71

Formula

C₁₁H₁₅ClN₄O₄

CAS No.

86583-19-9

SMILES

O[[email protected]]1[[email protected]@H](O[[email protected]](CO)[[email protected]]1O)N2C3=C(C(N)=NC=C3)N=C2.Cl

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
3-Deazaadenosine hydrochloride
Cat. No.:
HY-W013332A
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3-Deazaadenosine hydrochloride

Cat. No.: HY-W013332A