2. GPCR/G Protein Metabolic Enzyme/Protease
  3. EBI2/GPR183 Endogenous Metabolite
  4. 7α,25-Dihydroxycholesterol

7α,25-Dihydroxycholesterol  (Synonyms: 7α,25-OHC)

製品番号: HY-113962 純度: 99.88%
COA 取扱説明書 Technical Support

7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells.

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CAS 番号 : 64907-22-8

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 275 在庫あり
Solution
10 mM * 1 mL in DMSO USD 275 在庫あり
Solid
1 mg $55 在庫あり
5 mg $250 在庫あり
10 mg $480 在庫あり
25 mg $1060 在庫あり
50 mg $1850 在庫あり
100 mg $3250 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of 7α,25-Dihydroxycholesterol:

7α,25-Dihydroxycholesterol 関連抗体

Top Publications Citing Use of Products

    7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44.  [Abstract]

    A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell viability was assessed using CCK-8 assays at 24-, 48-, and 72-hour after treatment.

    7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44.  [Abstract]

    A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell apoptosis was quantified by flow cytometry using Annexin V-FITC/PI staining.

    7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44.  [Abstract]

    A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Transwell migration assays were used to quantify cell migration at 24 hours.

    7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44.  [Abstract]

    A549 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. The protein levels of ERK and Akt pathways related proteins, including phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt and total Akt, were analyzed by Immunoblotting.

    7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8.  [Abstract]

    Transwell migration of CD7+ and CD7− monocytes transfected with si-Scramble or si-Gpr183 to 7α,25-Dihydroxycholesterol (7α,25-OHC).

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

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    • 純度とドキュメンテーション

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    製品説明

    7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells[1][2].

    IC50 & Target[1]

    Human Endogenous Metabolite

     

    体外実験

    In vitro, 7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    7α,25-Dihydroxycholesterol 関連抗体
    体内実験

    EBI2-deficient B cells or normal B cells desensitized by 7α,25-Dihydroxycholesterol (1 μM; 1.5 hours) pre-treatment shows reduced homing to follicular areas of the spleen[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    418.65

    分子式

    C27H46O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)(O)C)([H])[C@@]3([H])[C@]([C@@]4(C(C[C@@H](O)CC4)=C[C@H]3O)C)([H])CC1
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 4.55 mg/mL (10.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : < 1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3886 mL 11.9432 mL 23.8863 mL
    5 mM 0.4777 mL 2.3886 mL 4.7773 mL
    10 mM 0.2389 mL 1.1943 mL 2.3886 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  15% Cremophor EL    85% Saline

      Solubility: 5 mg/mL (11.94 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.88%

    COA (249 KB) HNMR (144 KB) RP-HPLC (238 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3886 mL 11.9432 mL 23.8863 mL 59.7158 mL
    5 mM 0.4777 mL 2.3886 mL 4.7773 mL 11.9432 mL
    10 mM 0.2389 mL 1.1943 mL 2.3886 mL 5.9716 mL
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    7α,25-Dihydroxycholesterol Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    7α,25-Dihydroxycholesterol64907-22-87α,25-OHCEBI2/GPR183Endogenous MetaboliteendogenousligandGPCRreceptorBcellsTimmuneresponsemigrationoxysterolsInhibitorinhibitorinhibit

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    製品名:
    7α,25-Dihydroxycholesterol
    製品番号:
    HY-113962
    数量:
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