2. Apoptosis
  3. IAP
  4. BV6

BV6 is an antagonist of cIAP1 and XIAP, members of the inhibitors of apoptosis (IAP) family. BV6 shows an IC50 of 7.2 μM in MTS assays in HCC193 cells. BV6 can be used in the research of endometrial cancer and endometriosis.

For research use only. We do not sell to patients.

CAS No. : 1001600-56-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of BV6:

BV6 Related Antibodies

Top Publications Citing Use of Products

    BV6 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Mar 19;9(1):1136.   [Abstract]

    Western Blot analysis of p52, p100, NIK, c-IAP2, Peli1, and HSP60 in total lysis of control and Peli1-knockdown M12 cells that pretreated with DMSO or smac mimetic BV6 for 4 h, and then stimulated with anti-CD40 (αCD40) at the indicated time points.

    BV6 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Mar 19;9(1):1136.   [Abstract]

    Western Blot analysis of p52, p100, NIK, c-IAP2, Peli1, and HSP60 in total lysis of control and Peli1-knockdown M12 cells that pretreated with DMSO or smac mimetic BV6 for 4 h, and then stimulated with BAFF at the indicated time points.

    BV6 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Mar 19;9(1):1136.   [Abstract]

    Analysis of Lys48 ubiquitination of NIK in control and Peli1-knockdown M12 cells that pretreated with DMSO or smac mimetic BV6 for 4 h, then left unstimulated or stimulated with anti-CD40 (αCD40) for 4 h in the presence of MG132.

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    Description

    BV6 is an antagonist of cIAP1 and XIAP, members of the inhibitors of apoptosis (IAP) family. BV6 shows an IC50 of 7.2 μM in MTS assays in HCC193 cells. BV6 can be used in the research of endometrial cancer and endometriosis[1][2].

    IC50 & Target

    IAP[1]
    In Vitro

    BV6 (250 nM-30 μM, 24 h) induces apoptosis in HCC193 and H460 lung cancer cell lines in a concentration- and time-dependent manner. BV6 can also enhance the sensitivity of HCC193 and H460 cell lines to radiation[1].
    BV6 (1μM 24 h; 5 μM, 48 h) significantly sensitizes the cell to radiation (HCC193-DER=1.38, at 1 μM BV6; H460-DER=1.42, at 5 μM BV6)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BV6 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HCC193 cells, H460 cells
    Concentration: 250 nM, 500 nM, 1 μM, 3 μM, 5 μM, 10 μM, 20 μM, 30 μM
    Incubation Time: 24 hours
    Result: Decreased cell survival percentage for HCC193 with an IC50 of 7.2μM.
    Decreased the cell viability of H460 cells by about 60% at 30 μM.

    Western Blot Analysis[1]

    Cell Line: HCC193 cells, H460 cells
    Concentration: 0.25 μM, 0.5 μM, 1 μM, 5 μM
    Incubation Time: 1, 14, 24 hours
    Result: Reduced the expression of cIAP1 within one hour and gradually decreased XIAP with increased incubation time for both cell lines.
    Reduced the expression of cIAP1 with 0.25μM and gradually decreased XIAP with increasing concentration for both cell lines.

    ELISA Assay[1]

    Cell Line: HCC193 cells, H460 cells
    Concentration: 1 μM, 5 μM
    Incubation Time: 12 hours
    Result: Induced enhancement of the secretion of TNFα in HCC193 cells.
    Did not enhance the secretion of TNFα in H460 cells.
    In Vivo

    BV6 (10 mg/kg, twice weekly for 4 weeks, i.p.) attenuated the intensity of IAPs expression[2].
    BV6 (10 mg/kg, twice weekly for 4 weeks, i.p.) reduces the total number of lesions and the percentage of Ki67-positive cells compared to the control[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female endometriosis mice modeling by estradiol valerate (HY-B0672) at the dosage 0.5 µg/mouse/week s.c injected (6 weeks of age, BALB/c)[2].
    Dosage: 10 mg/kg
    Administration: a single i.p. injection,twice weekly for 4 weeks
    Result: Attenuated the intensity of IAPs expression.
    Molecular Weight

    1205.57

    Formula

    C70H96N10O8
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NCCCCCCNC([C@@H](NC([C@H](CCC1)N1C([C@H](C2CCCCC2)NC([C@H](C)NC)=O)=O)=O)C(C3=CC=CC=C3)C4=CC=CC=C4)=O)[C@@H](NC([C@H](CCC5)N5C([C@H](C6CCCCC6)NC([C@H](C)NC)=O)=O)=O)C(C7=CC=CC=C7)C8=CC=CC=C8
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (82.95 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8295 mL 4.1474 mL 8.2948 mL
    5 mM 0.1659 mL 0.8295 mL 1.6590 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.90%

    SDS (393 KB)

    COA (252 KB) HNMR (350 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.8295 mL 4.1474 mL 8.2948 mL 20.7371 mL
    5 mM 0.1659 mL 0.8295 mL 1.6590 mL 4.1474 mL
    10 mM 0.0829 mL 0.4147 mL 0.8295 mL 2.0737 mL
    15 mM 0.0553 mL 0.2765 mL 0.5530 mL 1.3825 mL
    20 mM 0.0415 mL 0.2074 mL 0.4147 mL 1.0369 mL
    25 mM 0.0332 mL 0.1659 mL 0.3318 mL 0.8295 mL
    30 mM 0.0276 mL 0.1382 mL 0.2765 mL 0.6912 mL
    40 mM 0.0207 mL 0.1037 mL 0.2074 mL 0.5184 mL
    50 mM 0.0166 mL 0.0829 mL 0.1659 mL 0.4147 mL
    60 mM 0.0138 mL 0.0691 mL 0.1382 mL 0.3456 mL
    80 mM 0.0104 mL 0.0518 mL 0.1037 mL 0.2592 mL
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    BV6 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BV61001600-56-1BV 6BV-6IAPcIAP1XIAPapoptosisHCC193Inhibitorinhibitorinhibit

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