CB-6644

Name: CB-6644
Brand: MedChemExpress
SKU: HY-114429
Rating: 5.0 (15 reviews)

Cat. No.: HY-114429 Purity: 98.76%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 2316817-88-4

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 15 publication(s) in Google Scholar

15 Publications Citing Use of MCE CB-6644

Bio/Physico-chemical Assay

In Vivo Efficacy Study

: CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]; The inhibitory effects of CB-6644 on the ATPase activity of human RUVBL1/2.

: CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]; Normalized luminescence rhythm of Bmal1-dLuc U2OS cells shows the period lengthening for 2 h in the presence of CB-6644 (3 μM), n = 3 biologically independent cells.

: CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]; Left panel: statistical analysis of the period length changes of Drosophila melanogaster. Right panel: represents of double-plotted averaged actogram of Drosophila melanogaster with CB-6644 or control treatment.

: CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]; Left panel: statistical analysis of the period length of Neurospora crassa with CB-6644 treatment. Right panel: normalized luminescence rhythm of Neurospora crassa treated by CB-6644.

: CB-6644 purchased from MedChemExpress. Usage Cited in: Nature. 2025 Jun;642(8066):165-173. [Abstract]; Left panel: statistical analysis of the period length of Arabidopsis thaliana with CB-6644 treatment. Right panel: normalized luminescence rhythm of Arabidopsis thaliana treated by CB-6644, cordycepin, and adenosine.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.3 μM Compound: 9; CB-6644	Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
HCT-116	IC₅₀	0.08 μM Compound: 9; CB-6644	Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
MDA-MB-231	IC₅₀	0.7 μM Compound: 9; CB-6644	Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]

In Vitro

CB-6644 (20 μM) interacts with the RUVBL1/2 complex in Ramos cells^[1].
CB-6644 (0.001-10 μM; 72 hours) potently kills 123 cell lines (including HCT116, NCI-1975, and HT29 cells) with an EC₅₀ range of 41 to 785 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	123 cell lines such as HCT116, NCI-1975, and HT29 cells
Concentration:	0.001, 0.01, 0.1, 1, and 10 μM
Incubation Time:	72 hours
Result:	Potently killed cells with an EC₅₀ range of 41 to 785 nM.

In Vivo

CB-6644 (150 mg/kg, oral administration, 10 days of treatment for SCID-beige mice bearing Ramos xenograft models, 30 days of treatment for SCID-beige mice bearing RPMI8226 xenograft models) has antitumor activity in xenograft tumor models with tumor growth inhibitions (TGIs) of 68 and 81%, respectively in Ramos xenograft models and RPMI8226 xenograft models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID-beige mice bearing human tumor xenografts derived from either Burkitt’s lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines^[1]
Dosage:	150 mg/kg
Administration:	Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226
Result:	There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.

분자량

573.06

화학식

C₂₉H₃₄ClFN₄O₅

CAS No.

2316817-88-4

Appearance

Solid

Color

White to light yellow

SMILES

COCC1=C(NC(CC(C)(C)CNC(C2=C(OCC)C=C(F)C(Cl)=C2)=O)=O)C(N(N1CCC3)C4=C3C=CC=C4)=O

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (174.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7450 mL	8.7251 mL	17.4502 mL
5 mM	0.3490 mL	1.7450 mL	3.4900 mL
10 mM	0.1745 mL	0.8725 mL	1.7450 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.85%

Select Batch:

Data Sheet (275 KB) SDS (394 KB)

COA (249 KB) HNMR (223 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. Assimon VA, et al. CB-6644 Is a Selective Inhibitor of the RUVBL1/2 Complex with Anticancer Activity. ACS Chem Biol. 2019 Feb 15;14(2):236-244. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7450 mL	8.7251 mL	17.4502 mL	43.6254 mL
	5 mM	0.3490 mL	1.7450 mL	3.4900 mL	8.7251 mL
	10 mM	0.1745 mL	0.8725 mL	1.7450 mL	4.3625 mL
	15 mM	0.1163 mL	0.5817 mL	1.1633 mL	2.9084 mL
	20 mM	0.0873 mL	0.4363 mL	0.8725 mL	2.1813 mL
	25 mM	0.0698 mL	0.3490 mL	0.6980 mL	1.7450 mL
	30 mM	0.0582 mL	0.2908 mL	0.5817 mL	1.4542 mL
	40 mM	0.0436 mL	0.2181 mL	0.4363 mL	1.0906 mL
	50 mM	0.0349 mL	0.1745 mL	0.3490 mL	0.8725 mL
	60 mM	0.0291 mL	0.1454 mL	0.2908 mL	0.7271 mL
	80 mM	0.0218 mL	0.1091 mL	0.2181 mL	0.5453 mL
	100 mM	0.0175 mL	0.0873 mL	0.1745 mL	0.4363 mL

CB-6644 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CB-66442316817-88-4CB6644CB 6644DNA/RNA SynthesisInhibitorinhibitorinhibit

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CB-6644

고객리뷰

CB-6644 Related Antibodies

15 Publications Citing Use of MCE CB-6644

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

CB-6644 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

CB-6644 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

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