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Forchlorfenuron 

Cat. No.: HY-B1841 Purity: 99.78%
Handling Instructions

Forchlorfenuron is plant growth regulator and cytokinin; can be used to increase fruit size of fruits, such as kiwi fruit and grapes.

For research use only. We do not sell to patients.

Forchlorfenuron Chemical Structure

Forchlorfenuron Chemical Structure

CAS No. : 68157-60-8

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Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Forchlorfenuron is plant growth regulator and cytokinin; can be used to increase fruit size of fruits, such as kiwi fruit and grapes.

In Vitro

Forchlorfenuron is a plant growth regulator, applied extensively to increase kiwifruit size and weight. Cytotoxicity of forchlorfenuron and its metabolites are tested through Sulforhodamine B assays against CHO cells. Forchlorfenuron exhibits significant cytotoxicity against CHO cells with an IC50 of 12.12±2.14 μM[1]. The half-lives offorchlorfenuron were 15.8-23.0 days, the final residues of forchlorfenuron in pulp were all ≤0.002 mg/kg, and most of the residues were concentrated in the peel. The risk assessment revealed that no significant potential health risk would be induced by forchlorfenuron in citrus fruits. Therefore, it could be safe to apply forchlorfenuron in citrus fruits[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

247.68

Formula

C₁₂H₁₀ClN₃O

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (403.75 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0375 mL 20.1873 mL 40.3747 mL
5 mM 0.8075 mL 4.0375 mL 8.0749 mL
10 mM 0.4037 mL 2.0187 mL 4.0375 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.09 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.09 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.09 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

CHO cells are cultured in high-glucose DMEM medium. Forchlorfenuron and its kiwifruit metabolites are dissolved in DMSO and diluted in culture media. Cells are seeded in a 96-well flat microtiter plate for 24 h and are then incubated for an additional 48 h with various concentrations of the compounds. Next, cells are fixed with 10% trichloroacetic acid and incubated for 60 min at 4 °C. The supernatant is discarded, then the plates are washed. Using 0.4% SRB solution dissolved in 1% acetic acid, the cell layer is strained, then the plates are incubated for 20 min at room temperature. The stained cells is solubilized in 10 mM un-buffered Tris base and optical density (OD) is measured at 560 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Product Name:
Forchlorfenuron
Cat. No.:
HY-B1841
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