2. Apoptosis Metabolic Enzyme/Protease
  3. Apoptosis Endogenous Metabolite
  4. Hematoporphyrin dihydrochloride

Hematoporphyrin dihydrochloride  (Synonyms: Hematoporphyrin IX dihydrochloride)

Cat. No.: HY-B0754A Pureza: 95.0%
COA
SDS
Instrucciones de manejo Technical Support

Hematoporphyrin dihydrochloride (Hematoporphyrin IX dihydrochloride), a photosensitizer, is a substrate for affinity chromatography of heme-binding proteins. Hematoporphyrin dihydrochloride can induce apoptosis in U87 glioma cells and decrease tumor growth in vivo when exposed to red light.

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Hematoporphyrin dihydrochloride

Hematoporphyrin dihydrochloride Estructura química

No. CAS : 17696-69-4

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Based on 15 publication(s) in Google Scholar

Other Forms of Hematoporphyrin dihydrochloride:

Hematoporphyrin dihydrochloride Related Antibodies

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Descripciòn

Hematoporphyrin dihydrochloride (Hematoporphyrin IX dihydrochloride), a photosensitizer, is a substrate for affinity chromatography of heme-binding proteins. Hematoporphyrin dihydrochloride can induce apoptosis in U87 glioma cells and decrease tumor growth in vivo when exposed to red light[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
Bel-7402 IC50
8.93 μM
Compound: HPD
Cytotoxicity against human Bel7402 cells after 48 hrs in the dark by MTT assay
Cytotoxicity against human Bel7402 cells after 48 hrs in the dark by MTT assay
[PMID: 19942325]
In Vitro

Hematoporphyrin (20-120 nM; 60 min) dose-dependently inhibits cell viability in U87 and U251 glioma cells, with IC50s of 85 and 166 nM, respectively[2].
? Hematoporphyrin (85 nM; 60 min) induces cell apoptosis via induction of ROS in U87 cells[2].
? Hematoporphyrin (85 nM; 60 min) induces morphological changes of U87 cells under the red light, including shrinking, fragmentation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hematoporphyrin dihydrochloride Related Antibodies

Cell Viability Assay[2]

Cell Line: U87 and U251 cells
Concentration: 20, 40, 60, 80, 100, 120 nM
Incubation Time: 60 min
Result: Inhibited cell viability in a dose-dependent manner.
Was more effective under the red light than white light.

Apoptosis Analysis[2]

Cell Line: U87 cells
Concentration: 85 nM
Incubation Time: 60 min
Result: Induced apoptotic nuclei in U87 cells with low cell density.
Induced the ROS and decreased the mitochondrial membrane potential.
In Vivo

Hematoporphyrin (5-10 mg/kg; i.p. for 2 months) with the irradiation of red light rapidly decreases the tumor size of rats, due to necrosis caused both by direct action of the photoactivated porphyrin on the tumor cells and by secondary effects on blood vessels[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar albino rats of both sexes (20 d; 60-80 g) bearing a subcutaneous solid Yoshida hepatoma AH-130[3]
Dosage: 5, 10 mg/kg
Administration: I.p. daily during the initial 10 days and biweekly for the next 2 months
Result: No tumor could be palpated a few days after exposure of the rats to light.
The skin healed completely and regrowth of the hair occurred.
Massive coagulation necrosis of the tumor 24 h after phototreatment (×40).
Peso molecular

671.61

Fòrmula

C34H40Cl2N4O6
No. CAS
Appearance

Solid

Color

Brown to black

SMILES

O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4)/C(C)=C(C(O)C)C4=C\C5=N/C(C(C(O)C)=C5C)=C\C(N2)=C1C)=N/3.Cl[H].Cl[H]
Structure Classification
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvente y solubilidad
In Vitro: 

DMSO : 116.67 mg/mL (173.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4890 mL 7.4448 mL 14.8896 mL
5 mM 0.2978 mL 1.4890 mL 2.9779 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Calculadora de molaridad

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 95.0%

SDS (394 KB)

COA (265 KB) HNMR (334 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4890 mL 7.4448 mL 14.8896 mL 37.2240 mL
5 mM 0.2978 mL 1.4890 mL 2.9779 mL 7.4448 mL
10 mM 0.1489 mL 0.7445 mL 1.4890 mL 3.7224 mL
15 mM 0.0993 mL 0.4963 mL 0.9926 mL 2.4816 mL
20 mM 0.0744 mL 0.3722 mL 0.7445 mL 1.8612 mL
25 mM 0.0596 mL 0.2978 mL 0.5956 mL 1.4890 mL
30 mM 0.0496 mL 0.2482 mL 0.4963 mL 1.2408 mL
40 mM 0.0372 mL 0.1861 mL 0.3722 mL 0.9306 mL
50 mM 0.0298 mL 0.1489 mL 0.2978 mL 0.7445 mL
60 mM 0.0248 mL 0.1241 mL 0.2482 mL 0.6204 mL
80 mM 0.0186 mL 0.0931 mL 0.1861 mL 0.4653 mL
100 mM 0.0149 mL 0.0744 mL 0.1489 mL 0.3722 mL
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Hematoporphyrin dihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hematoporphyrin17696-69-4Hematoporphyrin IXApoptosisEndogenous MetabolitephotosensitizerapoptosisgliomatumorredlightInhibitorinhibitorinhibit

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