1. Apoptosis
    Metabolic Enzyme/Protease
  2. Apoptosis
    Endogenous Metabolite
  3. Hematoporphyrin

Hematoporphyrin (Synonyms: Hematoporphyrin IX)

Cat. No.: HY-B0754 Purity: 95.81%
Handling Instructions

Hematoporphyrin (Hematoporphyrin IX), a photosensitizer, is a substrate for affinity chromatography of heme-binding proteins. Hematoporphyrin can induce apoptosis in U87 glioma cells and decrease tumor growth in vivo when exposed to red light.

For research use only. We do not sell to patients.

Hematoporphyrin Chemical Structure

Hematoporphyrin Chemical Structure

CAS No. : 14459-29-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
100 mg USD 72 Get quote
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Hematoporphyrin:

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Description

Hematoporphyrin (Hematoporphyrin IX), a photosensitizer, is a substrate for affinity chromatography of heme-binding proteins. Hematoporphyrin can induce apoptosis in U87 glioma cells and decrease tumor growth in vivo when exposed to red light[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Hematoporphyrin (20-120 nM; 60 min) dose-dependently inhibits cell viability in U87 and U251 glioma cells, with IC50s of 85 and 166 nM, respectively[2].
Hematoporphyrin (85 nM; 60 min) induces cell apoptosis via induction of ROS in U87 cells[2].
Hematoporphyrin (85 nM; 60 min) induces morphological changes of U87 cells under the red light, including shrinking, fragmentation[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: U87 and U251 cells
Concentration: 20, 40, 60, 80, 100, 120 nM
Incubation Time: 60 min
Result: Inhibited cell viability in a dose-dependent manner.
Was more effective under the red light than white light.

Apoptosis Analysis[2]

Cell Line: U87 cells
Concentration: 85 nM
Incubation Time: 60 min
Result: Induced apoptotic nuclei in U87 cells with low cell density.
Induced the ROS and decreased the mitochondrial membrane potential.
In Vivo

Hematoporphyrin (5-10 mg/kg; i.p. for 2 months) with the irradiation of red light rapidly decreases the tumor size of rats, due to necrosis caused both by direct action of the photoactivated porphyrin on the tumor cells and by secondary effects on blood vessels[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar albino rats of both sexes (20 d; 60-80 g) bearing a subcutaneous solid Yoshida hepatoma AH-130[3]
Dosage: 5, 10 mg/kg
Administration: I.p. daily during the initial 10 days and biweekly for the next 2 months
Result: No tumor could be palpated a few days after exposure of the rats to light.
The skin healed completely and regrowth of the hair occurred.
Massive coagulation necrosis of the tumor 24 h after phototreatment (×40).
Molecular Weight

598.69

Formula

C₃₄H₃₈N₄O₆

CAS No.

14459-29-1

SMILES

O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4)/C(C)=C(C(O)C)C4=C\C5=N/C(C(C(O)C)=C5C)=C\C(N2)=C1C)=N/3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (250.55 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6703 mL 8.3516 mL 16.7031 mL
5 mM 0.3341 mL 1.6703 mL 3.3406 mL
10 mM 0.1670 mL 0.8352 mL 1.6703 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.18 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References

Purity: 95.81%

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Keywords:

HematoporphyrinHematoporphyrin IXApoptosisEndogenous MetabolitephotosensitizerapoptosisgliomatumorredlightInhibitorinhibitorinhibit

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