IPTG (Synonyms: Isopropyl β-D-thiogalactoside)

Name: IPTG
Brand: MedChemExpress
SKU: HY-15921
Rating: 5.0 (33 reviews)

Cat. No.: HY-15921 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 367-93-1

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	해외재고보유	Estimated Time of Arrival: December 31
25 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 33 publication(s) in Google Scholar

33 Publications Citing Use of MCE IPTG

: IPTG purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 May 7;19(5). pii: E1393. [Abstract]; Screening of thymidylate synthase (TYMS) overexpression conditions.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

238.30

화학식

C₉H₁₈O₅S

CAS No.

367-93-1

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1SC(C)C

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

H₂O : 100 mg/mL (419.64 mM; Need ultrasonic)

DMSO : ≥ 60 mg/mL (251.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1964 mL	20.9820 mL	41.9639 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.93%

Select Batch:

Data Sheet (275 KB) SDS (557 KB)

COA (252 KB) HNMR (174 KB) RP-HPLC (240 KB) MS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Isopropyl β-D-1-thiogalactopyranoside

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.1964 mL	20.9820 mL	41.9639 mL	104.9098 mL
	5 mM	0.8393 mL	4.1964 mL	8.3928 mL	20.9820 mL
	10 mM	0.4196 mL	2.0982 mL	4.1964 mL	10.4910 mL
	15 mM	0.2798 mL	1.3988 mL	2.7976 mL	6.9940 mL
	20 mM	0.2098 mL	1.0491 mL	2.0982 mL	5.2455 mL
	25 mM	0.1679 mL	0.8393 mL	1.6786 mL	4.1964 mL
	30 mM	0.1399 mL	0.6994 mL	1.3988 mL	3.4970 mL
	40 mM	0.1049 mL	0.5245 mL	1.0491 mL	2.6227 mL
	50 mM	0.0839 mL	0.4196 mL	0.8393 mL	2.0982 mL
	60 mM	0.0699 mL	0.3497 mL	0.6994 mL	1.7485 mL
	80 mM	0.0525 mL	0.2623 mL	0.5245 mL	1.3114 mL
	100 mM	0.0420 mL	0.2098 mL	0.4196 mL	1.0491 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

IPTG Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IPTG367-93-1Isopropyl β-D-thiogalactosideBiochemical Assay ReagentsInhibitorinhibitorinhibit

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IPTG (Synonyms: Isopropyl β-D-thiogalactoside)

고객리뷰

IPTG Related Antibodies

33 Publications Citing Use of MCE IPTG

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

IPTG Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

IPTG Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report