Pembrolizumab (Synonyms: MK-3475; Lambrolizumab)

Name: Pembrolizumab
Brand: MedChemExpress
SKU: HY-P9902
Rating: 5.0 (30 reviews)

Art. -Nr.: HY-P9902 Reinheit: 99.74%: Data Sheet
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Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr. : 1374853-91-4

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Based on 30 publication(s) in Google Scholar

Pembrolizumab Spezialitätsempfehlungen:

30 Publications Citing Use of MCE Pembrolizumab

In Vivo Efficacy Study

Flow Cytometry

: Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]; Plots of tumor growth and tumor weight for each group (5 mice/group). The results showed that in mice, the combination of Pembrolizumab and Evolocumab significantly enhanced anti-tumor efficacy compared to Pembrolizumab alone (100 µg/mouse; i.p., every 7 days).

: Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 21;16(1):34. [Abstract]; Pembrolizumab (pembro) (5-10 µg/mL, 24 h). AMM16 cells were transfected with control siRNA (siRNA ctrl; left panels) or AR siRNA (siRNA AR; right panels) and treated with the indicated ICIs at 35 μg/ml. The red fluorescence from the propidium iodide (PI) and the green fluorescence from the acridin orange (AO) staining indicate dead and total cells, respectively.

: Pembrolizumab purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Aug 6;12(8):e009024. [Abstract]; The PD-1 expression of macrophages isolated from the subcutaneous tumor microenvironment of mice was detected by flow cytometry (n=5). The results showed that Pembrolizumab (anti-PD-1 Ab) (20 µg/mL, 24 h) reduced PD-1 expression.

: Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 May 9;12(5):465. [Abstract]; 4T1 tumor-bearing mice treated with anti-PD-1 mAbs (Pembrolizumab, 10 mg/kg; i.p.; twice a week) or LY2940002 (25 mg/kg; i.p.; twice a week) were euthanized at Day21 and the tumor volume were measured. The results showed that the administration of anti-PD-1 mAbs (Pembrolizumab), LY294002, or their combination significantly inhibited tumor growth in tumor-bearing mice.

: Pembrolizumab purchased from MedChemExpress. Usage Cited in: ACS Appl Bio Mater. 2020 Oct 19;3(10):7080-7086.; The immunofluorescence images of the tumor from the PBS- (A), random sequence- (B), anti-PD-1 antibody- (Pembrolizumab (5 mg/kg; s.c.; every other day for 16 days)) (C) and isotype antibody-treated group (D); The scale bar in the images is 50 µm.

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität
Reinheit & Dokumentation
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Beschreibung

Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

Isotype

Human IgG4 kappa

Recommend Isotype Controls

Human IgG4 (S228P) kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

PDCD1/PD-1/CD279

In Vitro

Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells^[2].
Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	αROR1-CAR T and A549 cells
Concentration:	10 μg/mL
Incubation Time:	Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result:	Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis^[2]

Cell Line:	A549 cells
Concentration:	10 μg/mL
Incubation Time:
Result:	Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.

In Vivo

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male BALB/c athymic nude mice^[2]
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

Klinische Studie

Gene ID

5133 [NCBI]

Accession

Q15116

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molekulargewicht

145.24 kDa

CAS. Nr.

1374853-91-4

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Pembrolizumab]

Versand

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG4 kappa

Biological Activity

Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Pembrolizumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
Loaded Pembrolizumab on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His, HY-P7396) with an affinity constant of 1.898E-09 M as determined in BLI assay.
Immobilized PD-1 Protein, Human can bind Pembrolizumab. The EC₅₀ for this effect is 1.8 ng/mL.

Reinheit & Dokumentation

Purity: 99.74%

Select Batch:

Data Sheet (263 KB) SDS (418 KB)

COA (232 KB) SDS-PAGE (95 KB) SEC-HPLC (72 KB)

Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Amita Patnaik, et al. Phase I Study of Pembrolizumab (MK-3475; Anti–PD-1 Monoclonal Antibody) in Patients with Advanced Solid Tumors. Clin Cancer Res.2015Oct1;21(19):4286-93. [Content Brief]

[2]. Zhenglin Ou, et al. Pressure increases PD‑L1 expression in A549 lung adenocarcinoma cells and causes resistance to anti‑ROR1 CAR T cell‑mediated cytotoxicity, Sci Rep [Content Brief]

[3]. Amita Patnaik, et al. Phase I Study of Pembrolizumab (MK-3475; Anti–PD-1 Monoclonal Antibody) in Patients with Advanced Solid Tumors. Clin Cancer Res.2015Oct1;21(19):4286-93.

[4]. Schachter J, et al. Pembrolizumab versus ipilimumab for advanced melanoma: final overall survival results of a multicentre, randomised, open-label phase 3 study (KEYNOTE-006). Lancet. 2017 Aug 16. pii: S0140-6736(17)31601-X. [Content Brief]

Pembrolizumab Related Classifications

Molaritätsrechner
Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Diese Gleichung wird häufig abgekürzt als: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pembrolizumab1374853-91-4MK-3475 LambrolizumabMK3475MK 3475PD-1/PD-L1PD-1/Programmed death-ligand 1PD-1 inhibitorIgG4 antibody cancerimmunologyInhibitorinhibitorinhibit

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Pembrolizumab (Synonyms: MK-3475; Lambrolizumab)

Kundenbewertung

Pembrolizumab Spezialitätsempfehlungen:

Pembrolizumab Related Antibodies

30 Publications Citing Use of MCE Pembrolizumab

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Pembrolizumab Related Antibodies

Pembrolizumab Related Classifications

Molaritätsrechner

Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

The dilution calculator equation

Keywords:

Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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