2. Immunology/Inflammation
  3. PD-1/PD-L1
  4. Pembrolizumab

Pembrolizumab  (Synonyms: MK-3475; Lambrolizumab)

製品番号: HY-P9902 純度: 99.74%
COA Technical Support

Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

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CAS 番号 : 1374853-91-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (100 μg)   今すぐ申し込む  
1 mg $280 在庫あり
5 mg $670 在庫あり
10 mg $910 在庫あり
25 mg $1600 在庫あり
50 mg $2560 在庫あり
100 mg $4000 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 30 publication(s) in Google Scholar

Pembrolizumab Featured Recommendations:

Pembrolizumab 関連抗体

Top Publications Citing Use of Products

    Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037.  [Abstract]

    Plots of tumor growth and tumor weight for each group (5 mice/group). The results showed that in mice, the combination of Pembrolizumab and Evolocumab significantly enhanced anti-tumor efficacy compared to Pembrolizumab alone (100 µg/mouse; i.p., every 7 days).

    Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 21;16(1):34.  [Abstract]

    Pembrolizumab (pembro) (5-10 µg/mL, 24 h). AMM16 cells were transfected with control siRNA (siRNA ctrl; left panels) or AR siRNA (siRNA AR; right panels) and treated with the indicated ICIs at 35 μg/ml. The red fluorescence from the propidium iodide (PI) and the green fluorescence from the acridin orange (AO) staining indicate dead and total cells, respectively.

    Pembrolizumab purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Aug 6;12(8):e009024.  [Abstract]

    The PD-1 expression of macrophages isolated from the subcutaneous tumor microenvironment of mice was detected by flow cytometry (n=5). The results showed that Pembrolizumab (anti-PD-1 Ab) (20 µg/mL, 24 h) reduced PD-1 expression.

    Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 May 9;12(5):465.  [Abstract]

    4T1 tumor-bearing mice treated with anti-PD-1 mAbs (Pembrolizumab, 10 mg/kg; i.p.; twice a week) or LY2940002 (25 mg/kg; i.p.; twice a week) were euthanized at Day21 and the tumor volume were measured. The results showed that the administration of​ anti-PD-1 mAbs (Pembrolizumab), LY294002, or their combination​ significantly inhibited tumor growth in tumor-bearing mice.

    Pembrolizumab purchased from MedChemExpress. Usage Cited in: ACS Appl Bio Mater. 2020 Oct 19;3(10):7080-7086.

    The immunofluorescence images of the tumor from the PBS- (A), random sequence- (B), anti-PD-1 antibody- (Pembrolizumab (5 mg/kg; s.c.; every other day for 16 days)) (C) and isotype antibody-treated group (D); The scale bar in the images is 50 µm.

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    製品説明

    Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

    Isotype

    Human IgG4 kappa
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target

    PDCD1/PD-1/CD279
    体外実験

    Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells[2].
    Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pembrolizumab 関連抗体

    Cell Cytotoxicity Assay[2]

    Cell Line: αROR1-CAR T and A549 cells
    Concentration: 10 μg/mL
    Incubation Time: Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
    Result: Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

    Apoptosis Analysis[2]

    Cell Line: A549 cells
    Concentration: 10 μg/mL
    Incubation Time:
    Result: Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.
    体内実験

    Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Six-week-old male BALB/c athymic nude mice[2]
    Dosage: 5 mg/kg
    Administration: Pembrolizumab (5 mg/kg; Intravenous injection; Once)
    Result: Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.
    臨床実験
    Gene ID

    5133  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    分子量

    145.24 kDa

    CAS 番号
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Pembrolizumab]
    輸送条件

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    保管条件

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG4 kappa
    Biological Activity
    • Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Pembrolizumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
    • Loaded Pembrolizumab on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His, HY-P7396) with an affinity constant of 1.898E-09 M as determined in BLI assay.
    • Immobilized PD-1 Protein, Human can bind Pembrolizumab. The EC50 for this effect is 1.8 ng/mL.
    純度とドキュメンテーション

    純度: 99.74%

    COA (232 KB) SDS-PAGE (95 KB) SEC-HPLC (72 KB)

    Inhibitory Antibodies User Guide (603 KB)
    参考文献
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Pembrolizumab Related Classifications

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pembrolizumab1374853-91-4MK-3475 LambrolizumabMK3475MK 3475PD-1/PD-L1PD-1/Programmed death-ligand 1PD-1 inhibitorIgG4 antibody cancerimmunologyInhibitorinhibitorinhibit

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    製品名:
    Pembrolizumab
    製品番号:
    HY-P9902
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