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  3. Methylene Blue

Methylene Blue  (Synonyms: Basic Blue 9; CI-52015; Methylthioninium chloride)

製品番号: HY-14536 純度: 95.05%
COA 取扱説明書 Technical Support

Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene Blue is a photosensitizer and redox agent. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.

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CAS 番号 : 61-73-4

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カスタマーレビュー

Based on 28 publication(s) in Google Scholar

Other Forms of Methylene Blue:

Top Publications Citing Use of Products

    Methylene Blue purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediates using 5'ETS-1, 5'ETS-2, 5'ETS and ITS1-29 probes in DDX10-AID mESCs with IAA treatment (0 h, 6 h, 24 h, and 48 h) or treated with IAA for 48 h followed by 48 h of washing. MB represents methylene blue staining. Experiments were repeated three times independently with similar results. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene Blue purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediate levels in DDX10-AID (+ OsTir1) mESCs overexpressing DDX10FL, DDX10ΔHBD, DDX10ΔHCD or DDX10ΔHD at 0 h and 48 h after IAA treatment. MB represents Methylene Blue staining. Experiments were repeated three times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene Blue purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediates in DDX10-AID (+ OsTir1) mESCs overexpressing NUP98-DDX10 or DDX10, treated with IAA at different time points (0 h, 24 h, and 48 h). MB represents Methylene Blue staining. Experiments were repeated two times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene Blue purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903.  [Abstract]

    Migration analysis of THP-1, co-cultured with cancer cells that were pre-treated with Methylene Blue.

    Methylene Blue purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903.  [Abstract]

    Western blotting analysis of PD-L1 expression in THP-1 which was co-cultured with cancer cells pre-treated with Methylene Blue.

    Monoamine Oxidase アイソフォーム固有の製品をすべて表示:

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    • 参考文献

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    製品説明

    Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene Blue is a photosensitizer and redox agent. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3][4].

    IC50 & Target

    Guanylyl cyclase (sGC)[1].
    Monoamine oxidase A (MAO-A)[1].
    NO synthase (NOS)[1]

    Cellular Effect
    Cell Line Type Value Description References
    B16-F10 IC50
    5.48 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    [PMID: 34871840]
    B16-F10 IC50
    7.02 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    B16-F10 IC50
    5.48 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    [PMID: 34871840]
    B16-F10 IC50
    7.02 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    B16-F10 IC50
    5.48 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
    [PMID: 34871840]
    B16-F10 IC50
    7.02 3
    Compound: MB
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    Fibroblast EC50
    43 1
    Compound: MB
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    [PMID: 33214825]
    HepG2 IC50
    6 3
    Compound: MB
    Anticancer activity against human HepG2 cells
    Anticancer activity against human HepG2 cells
    [PMID: 33460833]
    HepG2 IC50
    < 1 3
    Compound: Methylene blue
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    [PMID: 23927658]
    HepG2 IC50
    <1 3
    Compound: Methylene blue
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    [PMID: 23927658]
    HT-29 IC50
    > 10 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    [PMID: 32267685]
    HT-29 IC50
    >10 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    [PMID: 32267685]
    HT-29 IC50
    9.2 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    [PMID: 32267685]
    HT-29 IC50
    9.2 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    [PMID: 32267685]
    Lymphocyte EC50
    88 1
    Compound: MB
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    [PMID: 33214825]
    Fibroblast EC50
    43 1
    Compound: MB
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    [PMID: 33214825]
    MCF7 IC50
    10.58 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    [PMID: 34871840]
    MCF7 IC50
    14.64 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    MCF7 IC50
    10.58 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    [PMID: 34871840]
    MCF7 IC50
    14.64 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    FM3A EC50
    1.1 x 10-6 41
    Compound: Methylene blue
    Cytotoxicity against mouse mammary tumor FM3A cells representing a model of host
    Cytotoxicity against mouse mammary tumor FM3A cells representing a model of host
    [PMID: 11855978]
    Lymphocyte EC50
    88 1
    Compound: MB
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    [PMID: 33214825]
    Fibroblast EC50
    43 1
    Compound: MB
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
    [PMID: 33214825]
    HT-29 IC50
    9.2 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
    [PMID: 32267685]
    HT-29 IC50
    > 10 3
    Compound: MB
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
    [PMID: 32267685]
    HepG2 IC50
    6 3
    Compound: MB
    Anticancer activity against human HepG2 cells
    Anticancer activity against human HepG2 cells
    [PMID: 33460833]
    HepG2 IC50
    < 1 3
    Compound: Methylene blue
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
    [PMID: 23927658]
    Lymphocyte EC50
    88 1
    Compound: MB
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
    [PMID: 33214825]
    MCF7 IC50
    10.58 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
    [PMID: 34871840]
    MCF7 IC50
    14.64 3
    Compound: MB
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
    [PMID: 34871840]
    体外実験

    Methylene blue (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].
    1. Sample treatment:
    1.1 For paraffin sections: dewax and rehydrate as usual.
    1.2 For frozen sections: distilled water for 2 min.
    1.3 For cultured cells: Fix with 4% paraformaldehyde. Wash with distilled water for 2 min. Replace with fresh distilled water and wash for another 2 min.
    2. Methylene blue staining
    2.1 Stain with 0.1% Methylene blue for 2 min.
    2.2 Wash thoroughly with distilled water, observe and take photos.
    2.3 Staining results:
    Tissue or cells: blue

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    Methylene blue (Basic Blue 9) (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
    Methylene blue (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
    Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
    Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male NMRI mice[1]
    Dosage: 50 and 100 mg/kg
    Administration: Intraperitoneal injection; once, for 25 minutes
    Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
    Animal Model: CaMKIIα-tTA transactivator mice[2]
    Dosage: 20 and 40 mg/kg
    Administration: Oral administration; daily, for 6 months
    Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
    Animal Model: TBI-treated male BALB/c mice[3]
    Dosage: 2 mg/kg
    Administration: Intravenous injection; once, for 1 day
    Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
    Animal Model: TBI-treated male BALB/c mice[3]
    Dosage: 2 mg/kg
    Administration: Intravenous injection; once, for 1 day
    Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
    分子量

    319.85

    分子式

    C16H18ClN3S

    CAS 番号
    Appearance

    Solid

    Color

    Brown to reddish brown

    SMILES

    CN(C)C1=CC=C2C(SC(C(C=C/3)=N2)=CC3=[N+](C)/C)=C1.[Cl-]

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    RT, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (RT, sealed storage, away from moisture and light)

    溶剤 & 溶解度
    体外: 

    H2O : 50 mg/mL (156.32 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1265 mL 15.6323 mL 31.2647 mL
    5 mM 0.6253 mL 3.1265 mL 6.2529 mL
    10 mM 0.3126 mL 1.5632 mL 3.1265 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます:C1V1 = C2V2

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    純度とドキュメンテーション
    Dyeing Example
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 3.1265 mL 15.6323 mL 31.2647 mL 78.1616 mL
    5 mM 0.6253 mL 3.1265 mL 6.2529 mL 15.6323 mL
    10 mM 0.3126 mL 1.5632 mL 3.1265 mL 7.8162 mL
    15 mM 0.2084 mL 1.0422 mL 2.0843 mL 5.2108 mL
    20 mM 0.1563 mL 0.7816 mL 1.5632 mL 3.9081 mL
    25 mM 0.1251 mL 0.6253 mL 1.2506 mL 3.1265 mL
    30 mM 0.1042 mL 0.5211 mL 1.0422 mL 2.6054 mL
    40 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9540 mL
    50 mM 0.0625 mL 0.3126 mL 0.6253 mL 1.5632 mL
    60 mM 0.0521 mL 0.2605 mL 0.5211 mL 1.3027 mL
    80 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
    100 mM 0.0313 mL 0.1563 mL 0.3126 mL 0.7816 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    製品名:
    Methylene Blue
    製品番号:
    HY-14536
    数量:
    MCE 日本正規代理店: