2. Others Metabolic Enzyme/Protease
  3. Environmental Pollutants Endogenous Metabolite
  4. Vitamin A

Vitamin A  (Synonyms: Vitamin A1; all-trans-Retinol)

製品番号: HY-B1342 純度: 98.92%
COA 取扱説明書 Technical Support

Vitamin A (Vitamin A1) is an endogenous metabolite.

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Vitamin A

Vitamin A 構造式

CAS 番号 : 68-26-8

容量 価格（税別） 在庫状況 数量
5 mg $68 在庫あり
10 mg $90 在庫あり
25 mg $126 在庫あり
50 mg $164 在庫あり
100 mg $213 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of Vitamin A:

Vitamin A 関連抗体

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Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

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Human Endogenous Metabolite Microbial Metabolite

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製品説明

Vitamin A (Vitamin A1) is an endogenous metabolite.

IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
Fibroblast IC50
25 3
Compound: 2, retinol
Cytotoxicity against human normal fibroblast after 1 day by MTT assay
Cytotoxicity against human normal fibroblast after 1 day by MTT assay
[PMID: 18511283]
Fibroblast IC50
50 3
Compound: Retinol
Toxicity against human dermal fibroblasts
Toxicity against human dermal fibroblasts
[PMID: 19036585]
Panel NCI-60 (60 carcinoma cell lines) GI50
28 1
Compound: Vitamin A
Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program
Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program
[PMID: 15743190]
Fibroblast IC50
25 3
Compound: 2, retinol
Cytotoxicity against human normal fibroblast after 1 day by MTT assay
Cytotoxicity against human normal fibroblast after 1 day by MTT assay
[PMID: 18511283]
Fibroblast IC50
50 3
Compound: Retinol
Toxicity against human dermal fibroblasts
Toxicity against human dermal fibroblasts
[PMID: 19036585]
Panel NCI-60 (60 carcinoma cell lines) GI50
28 1
Compound: Vitamin A
Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program
Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program
[PMID: 15743190]
体外実験

It is found that contribution of hepatic microsomes (RDHs) to Retinol metabolism is greater than that of cytosol (ADHs), evidenced by higher Clint (Vmax/Km) of Vitamin A formation in microsomes than in cytosol[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Vitamin A 関連抗体
体内実験

The results show that compare with control (CON) rats, high-fat diet (HFD) significantly lowers basal level of Vitamin Al in plasma, but markedly elevates basal levels of Retinol in kidney, adipose tissue and liver. The results show that Vitamin Al absorption in HFD rats is faster than that in CON rats, evidenced by significantly shorter Tmax (3.0±0.0 h for HFD rats vs 5.8±1.1 h for CON rats, p<0.05)[1]. The plasma Vitamin A levels in methionine-choline deficient diet (MCD) rats are significantly lower than in the controls while the hepatic Vitamin A levels in MCD rats are markedly higher. The hepatic expression of Retinol-metabolizing enzymes and binding proteins (GRBP-I, ALDH1A1, and ALDH1A2) in MCD rats is significantly higher than that in control rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

286.45

分子式

C20H30O
CAS 番号
Appearance

Solid

Color

Off-white to yellow

SMILES

CC(/C=C/C=C(/C=C/C1=C(CCCC(C)1C)C)C)=C\CO
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.
溶剤 & 溶解度
体外: 

Ethanol : 100 mg/mL (349.10 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4910 mL 17.4551 mL 34.9101 mL
5 mM 0.6982 mL 3.4910 mL 6.9820 mL
10 mM 0.3491 mL 1.7455 mL 3.4910 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (17.46 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.27%

COA (241 KB) HNMR (244 KB) LCMS (94 KB)

取扱説明書 (2659 KB)
参考文献
キナーゼ実験
[1]

Activity of alcohol dehydrogenases (ADHs)/retinol dehydrogenases (RDHs) and retinal dehydrogenases (RALDHs) are assessed. Briefly, hepatic ADHs/RDHs and RALDHs activities are evaluated with Retinal formation in the presence of β-nicotinamide adenine dinucleotide (β-NAD+) and β-Nicotinamide adenine dinucleotide phosphate (β-NADP+), respectively. The incubation mixture consists of hepatic cytosol/microsomes (at the final concentration of 0.5 mg/mL), and a series concentrations of Retinol, 4.0 mM co-factor, 5 mM MgCl2, 91 μM butylated hydroxytoluene in a total volume of 500 μL. The reaction is initiated by the addition of Retinol pre-incubated for 5 min at 37°C. Following 15 min incubation, the reaction is quenched by extraction with equal volume of n-butanol/methanol, 95:5 (v:v) containing 0.005% butylated hydroxytoluene[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
動物実験
[2]

Wistar rats (male, aged 4 wk) are used in this study. Two groups of 6 rats are given free access to the following diets: the standard diet (control group) and the methionine-choline deficient diet (MCD) group. Each diet contains Retinol (1000 IU/100g). After 6 wk, the rats are sacrificed by exsanguination under isoflurane anesthesia after an overnight fast. Blood is collected in heparinized tubes, and plasma is separated for storage at -80°C. The liver, intestine, testes, and kidneys are removed, immediately frozen in liquid nitrogen, and stored at -80°C[2].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 3.4910 mL 17.4551 mL 34.9101 mL 87.2753 mL
5 mM 0.6982 mL 3.4910 mL 6.9820 mL 17.4551 mL
10 mM 0.3491 mL 1.7455 mL 3.4910 mL 8.7275 mL
15 mM 0.2327 mL 1.1637 mL 2.3273 mL 5.8184 mL
20 mM 0.1746 mL 0.8728 mL 1.7455 mL 4.3638 mL
25 mM 0.1396 mL 0.6982 mL 1.3964 mL 3.4910 mL
30 mM 0.1164 mL 0.5818 mL 1.1637 mL 2.9092 mL
40 mM 0.0873 mL 0.4364 mL 0.8728 mL 2.1819 mL
50 mM 0.0698 mL 0.3491 mL 0.6982 mL 1.7455 mL
60 mM 0.0582 mL 0.2909 mL 0.5818 mL 1.4546 mL
80 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0909 mL
100 mM 0.0349 mL 0.1746 mL 0.3491 mL 0.8728 mL
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Vitamin A Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vitamin A68-26-8Vitamin A1 all-trans-RetinolVitamin A 1Vitamin A-1Environmental PollutantsEndogenous MetaboliteEnvironmental ContaminantsInhibitorinhibitorinhibit

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