SRPIN340 (Synonyms: SRPK inhibitor)

Name: SRPIN340
Brand: MedChemExpress
SKU: HY-13949
Rating: 5.0 (16 reviews)

Cat. No.: HY-13949 Purity: 99.62%: Data Sheet
COA

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SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a K_i of 0.89 μM for SRPK1.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 218156-96-8

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 16 publication(s) in Google Scholar

16 Publications Citing Use of MCE SRPIN340

Cell Proliferation/Viability Assay

Microbiological Assay

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 11:e05479. [Abstract]; A549 cells were treated with SRPK1 kinase inhibitor SRPIN340 (10 µM) for 6 h, and then were infected by SeV for 12 h. The cellular lysates were used for cellular fractionation and immunoblotting with pAPI5. GAPDH and Histone H3 were markers of cytoplasmic elements (CE) and nuclear elements (NE), respectively. The results showed that the SRPK1-specific inhibitor SRPIN340 effectively blocked SeV-induced API5 phosphorylation.

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808. [Abstract]; Representative primary human type II pneumocyte culture images from samples that are pretreated with SRPIN340 (53 μM; 12 h) before they are infected with SARS-CoV-2. 24 hours later, the cells are fixed and stained for SARS-CoV-2, DNA with DAPI, and surfactant protein C (SFTPC). Scale bar, 100 μm.

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808. [Abstract]; Cellular viability (left axis) was measured after ACE2-A549 cells were treated with the indicated concentrations of SRPIN340 (2-53.5 μM) before infection. Data are from six independent replicates. SARS-CoV-2 viral RNA (right axis) was measured by qRT-PCR analysis 24 hours after infection. Data are from four independent biological replicates.

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808. [Abstract]; Infectious viral titers were measured by plaque assay of the supernatants of cells that were treated with SRPIN340 (53 μM) and infected for 48 hours.

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2021 Mar 18;49(5):2509-2521. [Abstract]; Induction of apoptosis in LAN-1,SK-N-BE(1) and SK-N-AS neuroblastoma cells (NB), but not in human glioma (GBM) cells (U87), osteosarcoma cells (U2OS) or normal human astrocytes (NHA) upon SRPIN340 treatment (20–50 μM) for 48 h, as detected by western blotting of full-length CASPASE-3, cleaved CASPASE-3, full-length PARP and cleaved PARP; β-ACTIN was used as a loading control. LAN-1 cells were included in the western blots for comparison.

: SRPIN340 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2019 Feb 26;510(1):97-103. [Abstract]; Bcl-2 protein expression and cleaved caspase-3 protein expression are analyzed by Western blot with or without the treatment of SRPIN340.

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a K_i of 0.89 μM for SRPK1.

IC₅₀ & Target

Ki: 0.89 μM (SRPK1)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	38.3 μM Compound: SRPIN340	Cytotoxicity against human HL60 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay	[PMID: 28407594]
Jurkat	IC₅₀	75.4 μM Compound: SRPIN340	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay	[PMID: 28407594]
NALM-6	IC₅₀	70.6 μM Compound: SRPIN340	Cytotoxicity against human NALM6 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay Cytotoxicity against human NALM6 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay	[PMID: 28407594]

In Vitro

SRPIN340 is a serine-arginine-rich protein kinase (SRPK) inhibitor, with a K_i of 0.89 μM for SRPK1. SRPIN340 also inhibits SRPK2, but shows no significant inhibition on other SRPK, such as Clk1 and Clk4. SRPIN340 promotes degradation of SRp75, which is necessary for HIV expression. SRPIN340 suppresses the propagation of Sindbis virus (IC₅₀, 60 μM) as well as severe acute respiratory syndrome virus^[1]. SRPIN340 shows inhibitory effect on leukemia cell lines, such as AML HL60, ALL-T Molt4 and Jurkat, with IC₅₀s of 44.7 μM, 92.2 μM and 82.3 μM, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

349.35

화학식

C₁₈H₁₈F₃N₃O

CAS No.

218156-96-8

Appearance

Solid

Color

White to light yellow

SMILES

O=C(NC1=CC(C(F)(F)F)=CC=C1N2CCCCC2)C3=CC=NC=C3

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : ≥ 42 mg/mL (120.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8625 mL	14.3123 mL	28.6246 mL
5 mM	0.5725 mL	2.8625 mL	5.7249 mL
10 mM	0.2862 mL	1.4312 mL	2.8625 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.62%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Fukuhara T, et al. Utilization of host SR protein kinases and RNA-splicing machinery during viral replication. Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11329-33. [Content Brief]

[2]. Siqueira RP, et al. Potential Antileukemia Effect and Structural Analyses of SRPK Inhibition by N-(2-(Piperidin-1-yl)-5-(Trifluoromethyl)Phenyl)Isonicotinamide (SRPIN340). PLoS One. 2015 Aug 5;10(8):e0134882. [Content Brief]

Cell Assay
^[2]

Leukemic cells (5 × 10⁴ cells/well) and isolated PBMCs (8 × 10⁴ cells/well) are seeded in 96-well plates. Each well contained 100 μL of complete RPMI medium and 100 μL of SRPIN340 solution at different concentrations. The compound is diluted in RPMI medium with 10% fetal bovine serum and 0.4% DMSO (v/v). After 48 h of culture, MTT (5 mg/mL) is added to the wells (3 h, 37°C). The plates are centrifuged at room temperature for 30 min 500 ×g, followed by the removal of the MTT solution and the addition of 100 μL/well of DMSO to solubilize the formazan. Absorbance is measured at 540 nm in a microplate reader. Each experimental procedure is performed in triplicate^[2].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Fukuhara T, et al. Utilization of host SR protein kinases and RNA-splicing machinery during viral replication. Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11329-33. [Content Brief]

[2]. Siqueira RP, et al. Potential Antileukemia Effect and Structural Analyses of SRPK Inhibition by N-(2-(Piperidin-1-yl)-5-(Trifluoromethyl)Phenyl)Isonicotinamide (SRPIN340). PLoS One. 2015 Aug 5;10(8):e0134882. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8625 mL	14.3123 mL	28.6246 mL	71.5615 mL
	5 mM	0.5725 mL	2.8625 mL	5.7249 mL	14.3123 mL
	10 mM	0.2862 mL	1.4312 mL	2.8625 mL	7.1561 mL
	15 mM	0.1908 mL	0.9542 mL	1.9083 mL	4.7708 mL
	20 mM	0.1431 mL	0.7156 mL	1.4312 mL	3.5781 mL
	25 mM	0.1145 mL	0.5725 mL	1.1450 mL	2.8625 mL
	30 mM	0.0954 mL	0.4771 mL	0.9542 mL	2.3854 mL
	40 mM	0.0716 mL	0.3578 mL	0.7156 mL	1.7890 mL
	50 mM	0.0572 mL	0.2862 mL	0.5725 mL	1.4312 mL
	60 mM	0.0477 mL	0.2385 mL	0.4771 mL	1.1927 mL
	80 mM	0.0358 mL	0.1789 mL	0.3578 mL	0.8945 mL
	100 mM	0.0286 mL	0.1431 mL	0.2862 mL	0.7156 mL

SRPIN340 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SRPIN340218156-96-8SRPK inhibitorSRPIN 340SRPIN-340SRPKVirus ProteaseSerine-arginine protein kinasesInhibitorinhibitorinhibit

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SRPIN340 (Synonyms: SRPK inhibitor)

고객리뷰

SRPIN340 Related Antibodies

16 Publications Citing Use of MCE SRPIN340

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

순도&문서

References

고객리뷰

SRPIN340 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

SRPIN340 Related Classifications

Keywords:

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