2. Cell Cycle/DNA Damage Anti-infection
  3. SRPK Virus Protease
  4. SRPIN340

SRPIN340  (Synonyms: SRPK inhibitor)

製品番号: HY-13949 純度: 99.62%
COA 取扱説明書 Technical Support

SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 218156-96-8

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 86 在庫あり
Solution
10 mM * 1 mL in DMSO USD 86 在庫あり
Solid
5 mg $79 在庫あり
10 mg $132 在庫あり
25 mg $280 在庫あり
50 mg $449 在庫あり
100 mg $581 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 16 publication(s) in Google Scholar

SRPIN340 関連抗体

Top Publications Citing Use of Products

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 11:e05479.  [Abstract]

    A549 cells were treated with SRPK1 kinase inhibitor SRPIN340 (10 µM) for 6 h, and then were infected by SeV for 12 h. The cellular lysates were used for cellular fractionation and immunoblotting with pAPI5. GAPDH and Histone H3 were markers of cytoplasmic elements (CE) and nuclear elements (NE), respectively. The results showed that the SRPK1-specific inhibitor SRPIN340 effectively blocked SeV-induced API5 phosphorylation.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808.  [Abstract]

    Representative primary human type II pneumocyte culture images from samples that are pretreated with SRPIN340 (53 μM; 12 h) before they are infected with SARS-CoV-2. 24 hours later, the cells are fixed and stained for SARS-CoV-2, DNA with DAPI, and surfactant protein C (SFTPC). Scale bar, 100 μm.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808.  [Abstract]

    Cellular viability (left axis) was measured after ACE2-A549 cells were treated with the indicated concentrations of SRPIN340 (2-53.5 μM) before infection. Data are from six independent replicates. SARS-CoV-2 viral RNA (right axis) was measured by qRT-PCR analysis 24 hours after infection. Data are from four independent biological replicates.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2022 Oct 25;15(757):eabm0808.  [Abstract]

    Infectious viral titers were measured by plaque assay of the supernatants of cells that were treated with SRPIN340 (53 μM) and infected for 48 hours.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2021 Mar 18;49(5):2509-2521.  [Abstract]

    Induction of apoptosis in LAN-1,SK-N-BE(1) and SK-N-AS neuroblastoma cells (NB), but not in human glioma (GBM) cells (U87), osteosarcoma cells (U2OS) or normal human astrocytes (NHA) upon SRPIN340 treatment (20–50 μM) for 48 h, as detected by western blotting of full-length CASPASE-3, cleaved CASPASE-3, full-length PARP and cleaved PARP; β-ACTIN was used as a loading control. LAN-1 cells were included in the western blots for comparison.

    SRPIN340 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2019 Feb 26;510(1):97-103.   [Abstract]

    Bcl-2 protein expression and cleaved caspase-3 protein expression are analyzed by Western blot with or without the treatment of SRPIN340.

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1.

    IC50 & Target

    Ki: 0.89 μM (SRPK1)[1]
    Cellular Effect
    Cell Line Type Value Description References
    HL-60 IC50
    38.3 μM
    Compound: SRPIN340
    Cytotoxicity against human HL60 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    Cytotoxicity against human HL60 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    		[PMID: 28407594]
    Jurkat IC50
    75.4 μM
    Compound: SRPIN340
    Cytotoxicity against human Jurkat cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    Cytotoxicity against human Jurkat cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    		[PMID: 28407594]
    NALM-6 IC50
    70.6 μM
    Compound: SRPIN340
    Cytotoxicity against human NALM6 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    Cytotoxicity against human NALM6 cells assessed as reduction in cell viability up to 200 uM after 48 hrs by MTT assay
    		[PMID: 28407594]
    体外実験

    SRPIN340 is a serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1. SRPIN340 also inhibits SRPK2, but shows no significant inhibition on other SRPK, such as Clk1 and Clk4. SRPIN340 promotes degradation of SRp75, which is necessary for HIV expression. SRPIN340 suppresses the propagation of Sindbis virus (IC50, 60 μM) as well as severe acute respiratory syndrome virus[1]. SRPIN340 shows inhibitory effect on leukemia cell lines, such as AML HL60, ALL-T Molt4 and Jurkat, with IC50s of 44.7 μM, 92.2 μM and 82.3 μM, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SRPIN340 関連抗体
    分子量

    349.35

    分子式

    C18H18F3N3O
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(NC1=CC(C(F)(F)F)=CC=C1N2CCCCC2)C3=CC=NC=C3
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 42 mg/mL (120.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8625 mL 14.3123 mL 28.6246 mL
    5 mM 0.5725 mL 2.8625 mL 5.7249 mL
    10 mM 0.2862 mL 1.4312 mL 2.8625 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.62%

    COA (252 KB) LCMS (94 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    Leukemic cells (5 × 104 cells/well) and isolated PBMCs (8 × 104 cells/well) are seeded in 96-well plates. Each well contained 100 μL of complete RPMI medium and 100 μL of SRPIN340 solution at different concentrations. The compound is diluted in RPMI medium with 10% fetal bovine serum and 0.4% DMSO (v/v). After 48 h of culture, MTT (5 mg/mL) is added to the wells (3 h, 37°C). The plates are centrifuged at room temperature for 30 min 500 ×g, followed by the removal of the MTT solution and the addition of 100 μL/well of DMSO to solubilize the formazan. Absorbance is measured at 540 nm in a microplate reader. Each experimental procedure is performed in triplicate[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8625 mL 14.3123 mL 28.6246 mL 71.5615 mL
    5 mM 0.5725 mL 2.8625 mL 5.7249 mL 14.3123 mL
    10 mM 0.2862 mL 1.4312 mL 2.8625 mL 7.1561 mL
    15 mM 0.1908 mL 0.9542 mL 1.9083 mL 4.7708 mL
    20 mM 0.1431 mL 0.7156 mL 1.4312 mL 3.5781 mL
    25 mM 0.1145 mL 0.5725 mL 1.1450 mL 2.8625 mL
    30 mM 0.0954 mL 0.4771 mL 0.9542 mL 2.3854 mL
    40 mM 0.0716 mL 0.3578 mL 0.7156 mL 1.7890 mL
    50 mM 0.0572 mL 0.2862 mL 0.5725 mL 1.4312 mL
    60 mM 0.0477 mL 0.2385 mL 0.4771 mL 1.1927 mL
    80 mM 0.0358 mL 0.1789 mL 0.3578 mL 0.8945 mL
    100 mM 0.0286 mL 0.1431 mL 0.2862 mL 0.7156 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    SRPIN340 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SRPIN340218156-96-8SRPK inhibitorSRPIN 340SRPIN-340SRPKVirus ProteaseSerine-arginine protein kinasesInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    SRPIN340
    製品番号:
    HY-13949
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.