TPI-1

Name: TPI-1
Brand: MedChemExpress
SKU: HY-100463
Rating: 5.0 (20 reviews)

Cat. No.: HY-100463 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

TPI-1, also known as Tyrosine Phosphatase Inhibitor 1, is a SHP-1 inhibitor; inhibits recombinant SHP-1 with an IC₅₀ of 40 nM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 79756-69-7

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 20 publication(s) in Google Scholar

20 Publications Citing Use of MCE TPI-1

RT-PCR

In Vivo Efficacy Study

ELISA

: TPI-1 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Apr 17;13(4):e010879. [Abstract]; Mice bearing YUMM 1.1 tumors were treated intraperitoneally (i.p.) with TPI-1 (3 mg/kg) in combination with ICI (anti-PD-1 and anti-CTLA), or singly with either TPI-1 or ICI, every 3 days beginning at the time of tumor visibility (day 7–10).

: TPI-1 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2024 Oct 29;20(10):e1012674. [Abstract]; Wild-type and VCP-deficient BMDM cells were pretreated with DMSO or the SHP1 inhibitor TPI-1 (10 μM) for 1 hour, then stimulated with ZymD at specified time points, followed by Western blot analysis.

: TPI-1 purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 Jun 10:13:865503. [Abstract]; TPI-1 (1 μg/mL). Supernatant was collected 18 hours post-infection, and cytokines were detected using a sandwich ELISA method.

: TPI-1 purchased from MedChemExpress. Usage Cited in: J Allergy Clin Immunol. 2022 Jan;149(1):223-236.e6. [Abstract]; TPI-1 (15 μM). Representative histograms of SHP-1 expression in WT and LAIR-1 KO ILC2s and their corresponding MFI quantification.

: TPI-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2020 Mar 9;17(1):81. [Abstract]; After overexpressing DJ-1 and inhibiting SHP-1 with virus and TPI-1 (10 ng/ml), respectively, the expression of NLRX1, TRAF6 and SHP-1 in rat brain tissue was detected by Western blotting.

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

TPI-1, also known as Tyrosine Phosphatase Inhibitor 1, is a SHP-1 inhibitor; inhibits recombinant SHP-1 with an IC₅₀ of 40 nM.

IC₅₀ & Target

IC50: 40 nM (recombinant SHP-1)^[1]

In Vitro

SHP-1 has been implicated as a potential cancer therapeutic target. TPI-1 is effective starting at 10 ng/mL in increasing SHP-1 phospho-substrates pLck-pY394. TPI-1 selectively increases SHP-1 phospho-substrates (pLck-pY394, pZap70 and pSlp76) in Jurkat T cells but has little effects on pERK1/2 or pLck-pY505. TPI-1 induces mouse splenic-IFNγ⁺ cells and induces IFNγ⁺ cells in human peripheral blood^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TPI-1 inhibits the growth of B16 melanoma tumors in mice at a tolerated oral dose in a T cell-dependent manner but has little effects on B16 cell growth in culture. TPI-1 thus also increases pLck-pY394 and IFNγ⁺ cells in mice. TPI-1 also inhibits B16 tumor growth and prolongs tumor mice survival as a tolerated s.c. agent^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

253.08

화학식

C₁₂H₆Cl₂O₂

CAS No.

79756-69-7

Appearance

Solid

Color

White to yellow

SMILES

ClC1=CC(C2=CC(C=CC2=O)=O)=C(Cl)C=C1

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 50 mg/mL (197.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9513 mL	19.7566 mL	39.5132 mL
5 mM	0.7903 mL	3.9513 mL	7.9026 mL
10 mM	0.3951 mL	1.9757 mL	3.9513 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 98.0%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (250 KB) HNMR (236 KB)

Handling Instructions (2659 KB)

References

[1]. Kundu S, et al. Novel SHP-1 inhibitors tyrosine phosphatase inhibitor-1 and analogs with preclinical anti-tumor activities as tolerated oral agents. J Immunol. 2010 Jun 1;184(11):6529-36. [Content Brief]

Animal Administration
^[1]

Mice: For in vivo induction of pLck-pY394 and IFNγ+ cells in mice, C57BL/6J mice are treated with PBS or TPI-1 (1 or 3 mg/kg, s.c.) for 4 days. Spleens are harvested one hour post-treatment on day 4 and processed into splenocytes, which are used for assessing pLck-pY394 levels by SDS-PAGE/Western blotting and for quantification of IFNγ+ cells by ELISPOT assays. Mice are also treated with TPI-1 (10 mg/kg, daily, s.c.) to evaluate the toxicity of the compounds in vivo^[1].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Kundu S, et al. Novel SHP-1 inhibitors tyrosine phosphatase inhibitor-1 and analogs with preclinical anti-tumor activities as tolerated oral agents. J Immunol. 2010 Jun 1;184(11):6529-36. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9513 mL	19.7566 mL	39.5132 mL	98.7830 mL
	5 mM	0.7903 mL	3.9513 mL	7.9026 mL	19.7566 mL
	10 mM	0.3951 mL	1.9757 mL	3.9513 mL	9.8783 mL
	15 mM	0.2634 mL	1.3171 mL	2.6342 mL	6.5855 mL
	20 mM	0.1976 mL	0.9878 mL	1.9757 mL	4.9391 mL
	25 mM	0.1581 mL	0.7903 mL	1.5805 mL	3.9513 mL
	30 mM	0.1317 mL	0.6586 mL	1.3171 mL	3.2928 mL
	40 mM	0.0988 mL	0.4939 mL	0.9878 mL	2.4696 mL
	50 mM	0.0790 mL	0.3951 mL	0.7903 mL	1.9757 mL
	60 mM	0.0659 mL	0.3293 mL	0.6586 mL	1.6464 mL
	80 mM	0.0494 mL	0.2470 mL	0.4939 mL	1.2348 mL
	100 mM	0.0395 mL	0.1976 mL	0.3951 mL	0.9878 mL

TPI-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPI-179756-69-7TPI1TPI 1SHP1PhosphataseShp1PTPN6Src homology region 2 (SH-2) domain-containing phosphatase 1Inhibitorinhibitorinhibit

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TPI-1

고객리뷰

TPI-1 Related Antibodies

20 Publications Citing Use of MCE TPI-1

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Protocol

순도&문서

References

고객리뷰

TPI-1 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

TPI-1 Related Classifications

Keywords:

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고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

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