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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1818
    Vari Fluor 680-Phalloidin
    Vari Fluor 680-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled at 680/700 nm.
    Vari Fluor 680-Phalloidin
  • HY-W013435
    1,2-Diaminoanthraquinone
    1,2-Diaminoanthraquinone is a sensitivity, specificity and nontoxic nitric oxide (NO) fluorescent probe. 1,2-Diaminoanthraquinone can be used to detect NO productions in live cell and animals with a maximum of absorption at about 540 nm and a detection limit of 5 μM for NO.
    1,2-Diaminoanthraquinone
  • HY-D2083
    BODIPY 540 (purity 99%)
    		99.32%
    BODIPY 540 (purity>98%) is a BODIPY dye.BODIPY dye is a small molecule dye with strong UV absorption ability.Its fluorescence peak is relatively sharp, its quantum yield is high, and it is relatively insensitive to the polarity and pH value of the environment.BODIPY 540 (purity>98%) has a purity higher than 98% and is suitable for cell experiments (Ex/Em = 540/580 nm).
    BODIPY 540 (purity 99%)
  • HY-114354
    BODIPY FL alkyne
    		98.60%
    BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease.
    BODIPY FL alkyne
  • HY-D1272
    Sulfo-Cy3 amine
    		98.03%
    Sulfo-Cy3 amine is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) bearing an amine group. The sulfonate ion increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The amine functionality of Sulfo-Cy3 amine can react with carboxyl groups to form covalent bonds. Sulfo-Cy3 amine can bind to biological molecules such as proteins and antibodies to track their location and dynamic changes in biological samples.
    Sulfo-Cy3 amine
  • HY-DY1046
    Green CMFDA (solution)
    Green CMFDA (solution) is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Solvent and concentration: DMSO: 10 mM
    Green CMFDA (solution)
  • HY-123749
    Tetramethylrhodamine-5-iodoacetamide
    Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments.
    Tetramethylrhodamine-5-iodoacetamide
  • HY-P3749
    Mca-(ala7,lys(dnp)9)-bradykinin
    		99.93%
    Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage.
    Mca-(ala7,lys(dnp)9)-bradykinin
  • HY-131021
    Janelia Fluor® 549, Azide
    		99.49%
    Janelia Fluor? 549, Azide (JF549, Azide) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    Janelia Fluor® 549, Azide
  • HY-W713926
    1-Pyrenedecanoic acid
    		Chemical 99.34%
    1-Pyrenedecanoic acid is a biochemical reagent, and can be used for membrane fluidity assessment.
    1-Pyrenedecanoic acid
  • HY-W800809
    FAM DBCO, 6-isomer
    		99.22%
    FAM DBCO, 6-isomer represents a variant of a time-honored fluorescent dye and featuring a terminal DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy.
    FAM DBCO, 6-isomer
  • HY-112624F
    CY7-Dextran (MW 4000)
    CY7-Dextran (MW 4000) is a fluorescent dye that consists of CY7 (HY-D0825) and Dextran (HY-112624) (Ex=740 nm; Em=770 nm). CY7-Dextran (MW 4000) can be used as a cell volume indicator and delineates the thin peripheral edges of the cells.
    CY7-Dextran (MW 4000)
  • HY-120601A
    Alizarin Red S
    Alizarin Red S (ARS) is an anthraquinone derivative dye. When combined with cations such as calcium ions, the functional group of Alizarin Red S can form a coordination bond with the cation through the oxygen atom to show orange-red fluorescence. Alizarin Red S can be used for screening of calcium compounds in synovial fluid and detecting osteoblast differentiation, and can also be used for bone staining in mice. Excitation/emission wavelength: 500/570 nm.
    Alizarin Red S
  • HY-P4417A
    Ac-IEPD-AMC TFA
    		99.33%
    Ac-IEPD-AMC TFA is a fluorescent substrate used to measure protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC fluoresces under UV light irradiation and can emit fluorescent signals.
    Ac-IEPD-AMC TFA
  • HY-168375
    BODIPY-Palmitate
    BODIPY-Palmitate is a fluorescently labeled palmitic acid (HY-N0830), with excitation/emission maxima at 488/508 nm. BODIPY-Palmitate can be used to detect fatty acid uptake and metabolism.
    BODIPY-Palmitate
  • HY-160250
    UGT1A1-IN-1
    		99.27%
    UGT1A1-IN-1 is a UGT1A1 inhibitor and fluorescent probe (Ex=370 nm, Em=520 nm), with an IC50 of 1.33 μM and a Ki of 5.02 μM. UGT1A1-IN-1 is selectively glucuronidated by UGT1A1 at the bilirubin homologous binding site, and its PET effect is blocked along with this reaction, triggering fluorescence changes. UGT1A1-IN-1 can serve as a substitute for bilirubin to detect UGT1A1 activity and perform high-throughput screening of UGT1A1 modulators.
    UGT1A1-IN-1
  • HY-D0784
    5-ROX
    		98.68%
    5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λexit of 580 nm (ε=3.6×104 M-1 cm-1), and λemit of 604 nm (=0.94).
    5-ROX
  • HY-D0721
    6-CFDA
    6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    6-CFDA
  • HY-D0012
    Bromothymol Blue
    		98.0%
    Bromothymol Blue is a pH indicator.
    Bromothymol Blue
  • HY-101893
    Dihydrofluorescein diacetate
    		98.48%
    Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.
    Dihydrofluorescein diacetate
Cat. No. Product Name / Synonyms Application Reactivity